Spatially mapping the tumour immune microenvironments of non-small cell lung cancer
Abstract Lung cancer is the leading cause of cancer-related deaths. An enhanced understanding of the immune microenvironments within these tumours may foster more precise and efficient treatment, particularly for immune-targeted therapies. The spatial architectural differences between lung adenocarc...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-02-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-025-56546-x |
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| author | Lysanne Desharnais Mark Sorin Morteza Rezanejad Bridget Liu Elham Karimi Aline Atallah Anikka M. Swaby Miranda W. Yu Samuel Doré Saskia Hartner Benoit Fiset Yuhong Wei Baharak Kadang Roni Rayes Philippe Joubert Sophie Camilleri-Broët Pierre-Olivier Fiset Daniela F. Quail Jonathan D. Spicer Logan A. Walsh |
| author_facet | Lysanne Desharnais Mark Sorin Morteza Rezanejad Bridget Liu Elham Karimi Aline Atallah Anikka M. Swaby Miranda W. Yu Samuel Doré Saskia Hartner Benoit Fiset Yuhong Wei Baharak Kadang Roni Rayes Philippe Joubert Sophie Camilleri-Broët Pierre-Olivier Fiset Daniela F. Quail Jonathan D. Spicer Logan A. Walsh |
| author_sort | Lysanne Desharnais |
| collection | DOAJ |
| description | Abstract Lung cancer is the leading cause of cancer-related deaths. An enhanced understanding of the immune microenvironments within these tumours may foster more precise and efficient treatment, particularly for immune-targeted therapies. The spatial architectural differences between lung adenocarcinoma (LUAD) and lung squamous cell carcinoma (LUSC) are relatively unexplored. Here, we applied imaging mass cytometry to a balanced cohort of LUAD and LUSC patients, matched for clinical factors such as age, sex, and smoking history, to analyze 204 histopathology images of tumours from 102 individuals with non-small cell lung cancer (NSCLC). By analyzing interactions and broader cellular networks, we interrogate the tumour microenvironment to understand how immune cells are spatially organized in clinically matched adenocarcinoma and squamous cell carcinoma subsets. This spatial analysis revealed distinct patterns of immune cell aggregation, particularly among macrophage populations, that correlated with patient prognosis differentially in adenocarcinoma and squamous cell carcinoma, suggesting potential new strategies for therapeutic intervention. Our findings underscore the importance of analyzing NSCLC histological subtypes separately when investigating the spatial immune landscape, as microenvironmental characteristics and cellular interactions differed by subtype. Recognizing these distinctions is essential for designing precision therapies tailored to each subtype’s unique immune architecture, ultimately enhancing patient outcomes. |
| format | Article |
| id | doaj-art-df57836258fb4823a052372cc6692e00 |
| institution | Kabale University |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-df57836258fb4823a052372cc6692e002025-02-09T12:44:16ZengNature PortfolioNature Communications2041-17232025-02-0116111210.1038/s41467-025-56546-xSpatially mapping the tumour immune microenvironments of non-small cell lung cancerLysanne Desharnais0Mark Sorin1Morteza Rezanejad2Bridget Liu3Elham Karimi4Aline Atallah5Anikka M. Swaby6Miranda W. Yu7Samuel Doré8Saskia Hartner9Benoit Fiset10Yuhong Wei11Baharak Kadang12Roni Rayes13Philippe Joubert14Sophie Camilleri-Broët15Pierre-Olivier Fiset16Daniela F. Quail17Jonathan D. Spicer18Logan A. Walsh19Rosalind and Morris Goodman Cancer Institute, McGill UniversityRosalind and Morris Goodman Cancer Institute, McGill UniversityRosalind and Morris Goodman Cancer Institute, McGill UniversityRosalind and Morris Goodman Cancer Institute, McGill UniversityRosalind and Morris Goodman Cancer Institute, McGill UniversityRosalind and Morris Goodman Cancer Institute, McGill UniversityRosalind and Morris Goodman Cancer Institute, McGill UniversityRosalind and Morris Goodman Cancer Institute, McGill UniversityRosalind and Morris Goodman Cancer Institute, McGill UniversityRosalind and Morris Goodman Cancer Institute, McGill UniversityRosalind and Morris Goodman Cancer Institute, McGill UniversityRosalind and Morris Goodman Cancer Institute, McGill UniversityDepartment of Pathology, McGill UniversityRosalind and Morris Goodman Cancer Institute, McGill UniversityDépartement de biologie moléculaire, biochimie médicale et de pathologie, Laval UniversityDepartment of Pathology, McGill UniversityDepartment of Pathology, McGill UniversityRosalind and Morris Goodman Cancer Institute, McGill UniversityRosalind and Morris Goodman Cancer Institute, McGill UniversityRosalind and Morris Goodman Cancer Institute, McGill UniversityAbstract Lung cancer is the leading cause of cancer-related deaths. An enhanced understanding of the immune microenvironments within these tumours may foster more precise and efficient treatment, particularly for immune-targeted therapies. The spatial architectural differences between lung adenocarcinoma (LUAD) and lung squamous cell carcinoma (LUSC) are relatively unexplored. Here, we applied imaging mass cytometry to a balanced cohort of LUAD and LUSC patients, matched for clinical factors such as age, sex, and smoking history, to analyze 204 histopathology images of tumours from 102 individuals with non-small cell lung cancer (NSCLC). By analyzing interactions and broader cellular networks, we interrogate the tumour microenvironment to understand how immune cells are spatially organized in clinically matched adenocarcinoma and squamous cell carcinoma subsets. This spatial analysis revealed distinct patterns of immune cell aggregation, particularly among macrophage populations, that correlated with patient prognosis differentially in adenocarcinoma and squamous cell carcinoma, suggesting potential new strategies for therapeutic intervention. Our findings underscore the importance of analyzing NSCLC histological subtypes separately when investigating the spatial immune landscape, as microenvironmental characteristics and cellular interactions differed by subtype. Recognizing these distinctions is essential for designing precision therapies tailored to each subtype’s unique immune architecture, ultimately enhancing patient outcomes.https://doi.org/10.1038/s41467-025-56546-x |
| spellingShingle | Lysanne Desharnais Mark Sorin Morteza Rezanejad Bridget Liu Elham Karimi Aline Atallah Anikka M. Swaby Miranda W. Yu Samuel Doré Saskia Hartner Benoit Fiset Yuhong Wei Baharak Kadang Roni Rayes Philippe Joubert Sophie Camilleri-Broët Pierre-Olivier Fiset Daniela F. Quail Jonathan D. Spicer Logan A. Walsh Spatially mapping the tumour immune microenvironments of non-small cell lung cancer Nature Communications |
| title | Spatially mapping the tumour immune microenvironments of non-small cell lung cancer |
| title_full | Spatially mapping the tumour immune microenvironments of non-small cell lung cancer |
| title_fullStr | Spatially mapping the tumour immune microenvironments of non-small cell lung cancer |
| title_full_unstemmed | Spatially mapping the tumour immune microenvironments of non-small cell lung cancer |
| title_short | Spatially mapping the tumour immune microenvironments of non-small cell lung cancer |
| title_sort | spatially mapping the tumour immune microenvironments of non small cell lung cancer |
| url | https://doi.org/10.1038/s41467-025-56546-x |
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