Iron overload and oxidative stress participated in zinc oxide nanoparticles-induced blood-brain barrier dysfunction
Since zinc oxide nanoparticles (ZnO-NPs) are widely used, concerns about their potential human health effects are growing. Although ZnO-NPs, due to their nano-size, can cross the blood-brain barrier (BBB) and affect brain function, the potential risk of ZnO-NPs in brain endothelial cells, the major...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-08-01
|
| Series: | Ecotoxicology and Environmental Safety |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651325008735 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Since zinc oxide nanoparticles (ZnO-NPs) are widely used, concerns about their potential human health effects are growing. Although ZnO-NPs, due to their nano-size, can cross the blood-brain barrier (BBB) and affect brain function, the potential risk of ZnO-NPs in brain endothelial cells, the major components of the BBB, is largely unknown. In brain endothelial cells (bEnd.3 cells), ZnO-NPs were exposed for 9 h to evaluate the brain endothelial dysfunction and BBB disruption. ZnO-NPs were deposited in the lysosome and promoted ferroptosis in bEnd.3 cells. Increased oxidative stress led to lysosomal dysfunction and cytotoxicity in bEnd cells treated with ZnO-NPs.3 cells. ZnO-NPs induced dysregulated autophagy flux and hyperpermeability via intracellular iron overload in bEnd.3 cells. We developed a putative adverse outcome pathway (AOP) to understand the effects of ZnO-NPs on the function of brain endothelial cells. Our study will help clarify the potential impact and toxicity of ZnO-NPs on the brain endothelium and the BBB. |
|---|---|
| ISSN: | 0147-6513 |