Protocol for tissue expansion microscopy for ultrastructure expansion of Xenopus embryos
Summary: Tissue expansion microscopy (TissUExM) allows super-resolution imaging by physically expanding biological samples. Here, we present a protocol for ultrastructure expansion microscopy of Xenopus embryos using TissUExM. We describe steps for tissue fixation, embedding, fluorescence labeling,...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-09-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166725003673 |
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| Summary: | Summary: Tissue expansion microscopy (TissUExM) allows super-resolution imaging by physically expanding biological samples. Here, we present a protocol for ultrastructure expansion microscopy of Xenopus embryos using TissUExM. We describe steps for tissue fixation, embedding, fluorescence labeling, and expansion. We demonstrate that our protocol provides enhanced resolution for studying subcellular structures, such as centrioles and cilia. This protocol has the potential to offer a broad range of applications to decipher sub-cellular organization in Xenopus embryos. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
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| ISSN: | 2666-1667 |