Target‐site mutations Ile1781Leu and Ile2041Asn in the ACCase2 gene confer resistance to fluazifop‐p‐butyl and pinoxaden herbicides in a johnsongrass accession from Arkansas, USA

Abstract Johnsongrass [Sorghum halepense (L.) Pers.] is a troublesome weed species in different agricultural and non‐agricultural areas. Because of its biology, reproductive system, and seed production, effective management is challenging. An accession with low susceptibility to the acetyl‐CoA carbo...

Full description

Saved in:
Bibliographic Details
Main Authors: Fidel González‐Torralva, Jason K. Norsworthy
Format: Article
Language:English
Published: Wiley 2024-03-01
Series:Plant Direct
Subjects:
Online Access:https://doi.org/10.1002/pld3.576
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1832541445007867904
author Fidel González‐Torralva
Jason K. Norsworthy
author_facet Fidel González‐Torralva
Jason K. Norsworthy
author_sort Fidel González‐Torralva
collection DOAJ
description Abstract Johnsongrass [Sorghum halepense (L.) Pers.] is a troublesome weed species in different agricultural and non‐agricultural areas. Because of its biology, reproductive system, and seed production, effective management is challenging. An accession with low susceptibility to the acetyl‐CoA carboxylase (ACCase)‐inhibiting herbicides fluazifop‐p‐butyl (fluazifop) and pinoxaden was collected in eastern Arkansas. In this research, the molecular mechanisms responsible for ACCase resistance were investigated. Dose–response experiments showed a resistance factor of 181 and 133 for fluazifop and pinoxaden, respectively. Molecular analysis of both ACCase1 and ACCase2 genes was researched. Nucleotide comparison of ACCase1 between resistant and susceptible accessions showed no single nucleotide polymorphisms. Nonetheless, analysis of ACCase2 in fluazifop‐resistant johnsongrass plants revealed the Ile1781Leu target‐site mutation was dominant (nearly 75%), whereas the majority of pinoxaden‐resistant johnsongrass plants had the Ile2041Asn (60%). Not all sequenced johnsongrass plants displayed a target‐site mutation, suggesting the presence of additional resistance mechanisms. Amplification of ACCase1 and ACCase2 was not responsible for resistance because of the similar values obtained in both resistant and susceptible accessions. Experiments with malathion and NBD‐Cl suggest the presence of herbicide metabolism. Outcomes of this research demonstrated that fluazifop‐ and pinoxaden‐resistant johnsongrass plants displayed a target‐site mutation in ACCase2, but also that non‐target‐site resistance mechanisms would be involved and require a detailed study.
format Article
id doaj-art-df4d8205975045bfa024114cfa5745fb
institution Kabale University
issn 2475-4455
language English
publishDate 2024-03-01
publisher Wiley
record_format Article
series Plant Direct
spelling doaj-art-df4d8205975045bfa024114cfa5745fb2025-02-04T08:31:05ZengWileyPlant Direct2475-44552024-03-0183n/an/a10.1002/pld3.576Target‐site mutations Ile1781Leu and Ile2041Asn in the ACCase2 gene confer resistance to fluazifop‐p‐butyl and pinoxaden herbicides in a johnsongrass accession from Arkansas, USAFidel González‐Torralva0Jason K. Norsworthy1Department of Crop, Soil, and Environmental Sciences University of Arkansas Fayetteville AR USADepartment of Crop, Soil, and Environmental Sciences University of Arkansas Fayetteville AR USAAbstract Johnsongrass [Sorghum halepense (L.) Pers.] is a troublesome weed species in different agricultural and non‐agricultural areas. Because of its biology, reproductive system, and seed production, effective management is challenging. An accession with low susceptibility to the acetyl‐CoA carboxylase (ACCase)‐inhibiting herbicides fluazifop‐p‐butyl (fluazifop) and pinoxaden was collected in eastern Arkansas. In this research, the molecular mechanisms responsible for ACCase resistance were investigated. Dose–response experiments showed a resistance factor of 181 and 133 for fluazifop and pinoxaden, respectively. Molecular analysis of both ACCase1 and ACCase2 genes was researched. Nucleotide comparison of ACCase1 between resistant and susceptible accessions showed no single nucleotide polymorphisms. Nonetheless, analysis of ACCase2 in fluazifop‐resistant johnsongrass plants revealed the Ile1781Leu target‐site mutation was dominant (nearly 75%), whereas the majority of pinoxaden‐resistant johnsongrass plants had the Ile2041Asn (60%). Not all sequenced johnsongrass plants displayed a target‐site mutation, suggesting the presence of additional resistance mechanisms. Amplification of ACCase1 and ACCase2 was not responsible for resistance because of the similar values obtained in both resistant and susceptible accessions. Experiments with malathion and NBD‐Cl suggest the presence of herbicide metabolism. Outcomes of this research demonstrated that fluazifop‐ and pinoxaden‐resistant johnsongrass plants displayed a target‐site mutation in ACCase2, but also that non‐target‐site resistance mechanisms would be involved and require a detailed study.https://doi.org/10.1002/pld3.576ACCaseherbicide resistancejohnsongrass, target‐site mutation
spellingShingle Fidel González‐Torralva
Jason K. Norsworthy
Target‐site mutations Ile1781Leu and Ile2041Asn in the ACCase2 gene confer resistance to fluazifop‐p‐butyl and pinoxaden herbicides in a johnsongrass accession from Arkansas, USA
Plant Direct
ACCase
herbicide resistance
johnsongrass, target‐site mutation
title Target‐site mutations Ile1781Leu and Ile2041Asn in the ACCase2 gene confer resistance to fluazifop‐p‐butyl and pinoxaden herbicides in a johnsongrass accession from Arkansas, USA
title_full Target‐site mutations Ile1781Leu and Ile2041Asn in the ACCase2 gene confer resistance to fluazifop‐p‐butyl and pinoxaden herbicides in a johnsongrass accession from Arkansas, USA
title_fullStr Target‐site mutations Ile1781Leu and Ile2041Asn in the ACCase2 gene confer resistance to fluazifop‐p‐butyl and pinoxaden herbicides in a johnsongrass accession from Arkansas, USA
title_full_unstemmed Target‐site mutations Ile1781Leu and Ile2041Asn in the ACCase2 gene confer resistance to fluazifop‐p‐butyl and pinoxaden herbicides in a johnsongrass accession from Arkansas, USA
title_short Target‐site mutations Ile1781Leu and Ile2041Asn in the ACCase2 gene confer resistance to fluazifop‐p‐butyl and pinoxaden herbicides in a johnsongrass accession from Arkansas, USA
title_sort target site mutations ile1781leu and ile2041asn in the accase2 gene confer resistance to fluazifop p butyl and pinoxaden herbicides in a johnsongrass accession from arkansas usa
topic ACCase
herbicide resistance
johnsongrass, target‐site mutation
url https://doi.org/10.1002/pld3.576
work_keys_str_mv AT fidelgonzaleztorralva targetsitemutationsile1781leuandile2041asnintheaccase2geneconferresistancetofluazifoppbutylandpinoxadenherbicidesinajohnsongrassaccessionfromarkansasusa
AT jasonknorsworthy targetsitemutationsile1781leuandile2041asnintheaccase2geneconferresistancetofluazifoppbutylandpinoxadenherbicidesinajohnsongrassaccessionfromarkansasusa