Revalorisation of brewer’s spent grain for biotechnological production of hydrogen with Escherichia coli
IntroductionAgro-industrial wastes are generated in huge amounts triggering damages to the environment and human health. Therefore, there is an urgent necessity for its revalorisation into high-value compounds, including biofuels. One such wastes is the brewer's spent grain (BSG), a by-product...
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Frontiers Media S.A.
2024-11-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2024.1473704/full |
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| author | Gema Cabrera Gema Cabrera José Manuel Jáimez Jezabel Sánchez-Oneto Jezabel Sánchez-Oneto Jorge Bolivar Jorge Bolivar Antonio Valle Antonio Valle |
| author_facet | Gema Cabrera Gema Cabrera José Manuel Jáimez Jezabel Sánchez-Oneto Jezabel Sánchez-Oneto Jorge Bolivar Jorge Bolivar Antonio Valle Antonio Valle |
| author_sort | Gema Cabrera |
| collection | DOAJ |
| description | IntroductionAgro-industrial wastes are generated in huge amounts triggering damages to the environment and human health. Therefore, there is an urgent necessity for its revalorisation into high-value compounds, including biofuels. One such wastes is the brewer's spent grain (BSG), a by-product of the beer industry, which is produced in vast quantities worldwide. The rich-fibre and protein content of BSG makes this waste a valuable resource for biotechnological applications, although the main challenge of this approach is to make the carbohydrates and proteins available for bacterial metabolisation into high-value products. This work aims to optimise a thermal-hydrolysis process to revalorise BSG by bacterial conversion into hydrogen (H2), as a clean energy that can replace fossil fuels.MethodsA 2k full factorial design method was employed hydrolysation of BSG and showed that temperature and acid concentration are significant factors that affect the extraction of reducing sugars (RS) and proteins. Subsequently, steepest ascent and central composite design (CCD) statistical methods were applied to determine the optimal conditions for hydrolysis.ResultsThe optimised hydrolysis condition were 0.047 M H2SO4, 150°C, 30 min and 15% BSG, leading to the theoretical concentrations of 54.8 g RS/L and 20 g/L proteins. However, 5'-hydroxymethylfurfural (HMF) was generated in thermal-hydrolysis conditions at higher temperatures exceeding 132°C. Therefore, a screening of HBSGs fermentation using Escherichia coli was conducted in order to identify the most suitable conditions for maximizing H2, as well as the production of volatile fatty acids (succinate and acetate) and ethanol. Among the tested conditions, HBSG A17 (117°C, 20 min, and 0.1 M H2SO4) yielded the highest H2 production of 48 mmol/L in this work.ConclusionThis study provides valuable insights into the optimisation of BSG pre-treatment for biotechnological applications, which may help in the selection of the most appropriate hydrolysis conditions based on the desired end product. |
| format | Article |
| id | doaj-art-df410894f66e402ca28bbd080c78678f |
| institution | OA Journals |
| issn | 2296-4185 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj-art-df410894f66e402ca28bbd080c78678f2025-08-20T02:23:46ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852024-11-011210.3389/fbioe.2024.14737041473704Revalorisation of brewer’s spent grain for biotechnological production of hydrogen with Escherichia coliGema Cabrera0Gema Cabrera1José Manuel Jáimez2Jezabel Sánchez-Oneto3Jezabel Sánchez-Oneto4Jorge Bolivar5Jorge Bolivar6Antonio Valle7Antonio Valle8Department of Chemical Engineering and Food Technology, Campus Universitario de Puerto Real, University of Cadiz, Cadiz, SpainInstitute of Viticulture and Agri-Food Research (IVAGRO), International Campus of Excellence (ceiA3), University of Cadiz, Cadiz, SpainDepartment of Chemical Engineering and Food Technology, Campus Universitario de Puerto Real, University of Cadiz, Cadiz, SpainDepartment of Chemical Engineering and Food Technology, Campus Universitario de Puerto Real, University of Cadiz, Cadiz, SpainInstitute of Viticulture and Agri-Food Research (IVAGRO), International Campus of Excellence (ceiA3), University of Cadiz, Cadiz, SpainDepartment of Biomedicine, Biotechnology and Public Health-Biochemistry and Molecular Biology, Campus Universitario de Puerto Real, University of Cadiz, Cadiz, SpainInstitute of Biomolecules (INBIO), University of Cadiz, Cadiz, SpainInstitute of Viticulture and Agri-Food Research (IVAGRO), International Campus of Excellence (ceiA3), University of Cadiz, Cadiz, SpainDepartment of Biomedicine, Biotechnology and Public Health-Biochemistry and Molecular Biology, Campus Universitario de Puerto Real, University of Cadiz, Cadiz, SpainIntroductionAgro-industrial wastes are generated in huge amounts triggering damages to the environment and human health. Therefore, there is an urgent necessity for its revalorisation into high-value compounds, including biofuels. One such wastes is the brewer's spent grain (BSG), a by-product of the beer industry, which is produced in vast quantities worldwide. The rich-fibre and protein content of BSG makes this waste a valuable resource for biotechnological applications, although the main challenge of this approach is to make the carbohydrates and proteins available for bacterial metabolisation into high-value products. This work aims to optimise a thermal-hydrolysis process to revalorise BSG by bacterial conversion into hydrogen (H2), as a clean energy that can replace fossil fuels.MethodsA 2k full factorial design method was employed hydrolysation of BSG and showed that temperature and acid concentration are significant factors that affect the extraction of reducing sugars (RS) and proteins. Subsequently, steepest ascent and central composite design (CCD) statistical methods were applied to determine the optimal conditions for hydrolysis.ResultsThe optimised hydrolysis condition were 0.047 M H2SO4, 150°C, 30 min and 15% BSG, leading to the theoretical concentrations of 54.8 g RS/L and 20 g/L proteins. However, 5'-hydroxymethylfurfural (HMF) was generated in thermal-hydrolysis conditions at higher temperatures exceeding 132°C. Therefore, a screening of HBSGs fermentation using Escherichia coli was conducted in order to identify the most suitable conditions for maximizing H2, as well as the production of volatile fatty acids (succinate and acetate) and ethanol. Among the tested conditions, HBSG A17 (117°C, 20 min, and 0.1 M H2SO4) yielded the highest H2 production of 48 mmol/L in this work.ConclusionThis study provides valuable insights into the optimisation of BSG pre-treatment for biotechnological applications, which may help in the selection of the most appropriate hydrolysis conditions based on the desired end product.https://www.frontiersin.org/articles/10.3389/fbioe.2024.1473704/fullbrewer’s spent grainbiohydrogenEscherichia colidesign of experimentdark fermentation |
| spellingShingle | Gema Cabrera Gema Cabrera José Manuel Jáimez Jezabel Sánchez-Oneto Jezabel Sánchez-Oneto Jorge Bolivar Jorge Bolivar Antonio Valle Antonio Valle Revalorisation of brewer’s spent grain for biotechnological production of hydrogen with Escherichia coli Frontiers in Bioengineering and Biotechnology brewer’s spent grain biohydrogen Escherichia coli design of experiment dark fermentation |
| title | Revalorisation of brewer’s spent grain for biotechnological production of hydrogen with Escherichia coli |
| title_full | Revalorisation of brewer’s spent grain for biotechnological production of hydrogen with Escherichia coli |
| title_fullStr | Revalorisation of brewer’s spent grain for biotechnological production of hydrogen with Escherichia coli |
| title_full_unstemmed | Revalorisation of brewer’s spent grain for biotechnological production of hydrogen with Escherichia coli |
| title_short | Revalorisation of brewer’s spent grain for biotechnological production of hydrogen with Escherichia coli |
| title_sort | revalorisation of brewer s spent grain for biotechnological production of hydrogen with escherichia coli |
| topic | brewer’s spent grain biohydrogen Escherichia coli design of experiment dark fermentation |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2024.1473704/full |
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