Modified stereotactic neurosurgery techniques for rodent surgery enhance survival and reduce surgery time in a severe traumatic brain injury model
Abstract Controlled cortical impact (CCI) is the most widely used mechanical model of traumatic brain injury (TBI) in rodent brains. This neurosurgical procedure generally involves the use of a stereotaxic system, which requires reaching a specific brain region with the most accurate position possib...
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Nature Portfolio
2025-07-01
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| Online Access: | https://doi.org/10.1038/s41598-025-05328-y |
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| author | Pongsakorn Wechakarn Sumeth Klomchitcharoen Tirapoot Jatupornpoonsub Nantawachara Jirakittayakorn Chanon Puttanawarut Wanlop Likitsuntonwong Khuanjit Chaimongkolnukul Yodchanan Wongsawat |
| author_facet | Pongsakorn Wechakarn Sumeth Klomchitcharoen Tirapoot Jatupornpoonsub Nantawachara Jirakittayakorn Chanon Puttanawarut Wanlop Likitsuntonwong Khuanjit Chaimongkolnukul Yodchanan Wongsawat |
| author_sort | Pongsakorn Wechakarn |
| collection | DOAJ |
| description | Abstract Controlled cortical impact (CCI) is the most widely used mechanical model of traumatic brain injury (TBI) in rodent brains. This neurosurgical procedure generally involves the use of a stereotaxic system, which requires reaching a specific brain region with the most accurate position possible. In this study, a modified stereotaxic system for TBI induction was developed to evaluate preclinical research in rodents for conducting neural stimulation experiments by using an implanted electrode to assist in rehabilitation after severe TBI. The proposed model aims to reduce animal mortality during surgery and alleviate the negative side effects potentially caused by prolonged anesthesia drug usage. Isoflurane is applied as an anesthetic drug before stereotaxic surgery in rodents, which promotes hypothermia in the animal body. The result showed notable improvement in rodent survival after applying an active warming pad system to prevent hypothermia. Compared with the conventional stereotaxic system, the modified CCI device with a mounted 3D-printed header significantly improved performance in the surgical procedure, decreasing the total operation time by 21.7%, especially in the Bregma‒Lambda measurement. These findings indicate the tangible capability of our modified stereotaxic system, which allows surgeons to perform stereotaxic surgery faster and lowers the risk of intraoperative mortality. |
| format | Article |
| id | doaj-art-df401bbb85904ce1860c7b6dfd1239ab |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-df401bbb85904ce1860c7b6dfd1239ab2025-08-20T03:37:29ZengNature PortfolioScientific Reports2045-23222025-07-0115111010.1038/s41598-025-05328-yModified stereotactic neurosurgery techniques for rodent surgery enhance survival and reduce surgery time in a severe traumatic brain injury modelPongsakorn Wechakarn0Sumeth Klomchitcharoen1Tirapoot Jatupornpoonsub2Nantawachara Jirakittayakorn3Chanon Puttanawarut4Wanlop Likitsuntonwong5Khuanjit Chaimongkolnukul6Yodchanan Wongsawat7Brain-Computer Interface Laboratory, Department of Biomedical Engineering, Faculty of Engineering, Mahidol UniversityBrain-Computer Interface Laboratory, Department of Biomedical Engineering, Faculty of Engineering, Mahidol UniversityDepartment of Prosthodontics, Faculty of Dentistry, Mahidol UniversityBrain-Computer Interface Laboratory, Department of Biomedical Engineering, Faculty of Engineering, Mahidol UniversityDepartment of Clinical Epidemiology and Biostatistics, Faculty of Medicine Ramathibodi Hospital, Mahidol UniversityNational Laboratory Animal Center, Mahidol UniversityNational Laboratory Animal Center, Mahidol UniversityBrain-Computer Interface Laboratory, Department of Biomedical Engineering, Faculty of Engineering, Mahidol UniversityAbstract Controlled cortical impact (CCI) is the most widely used mechanical model of traumatic brain injury (TBI) in rodent brains. This neurosurgical procedure generally involves the use of a stereotaxic system, which requires reaching a specific brain region with the most accurate position possible. In this study, a modified stereotaxic system for TBI induction was developed to evaluate preclinical research in rodents for conducting neural stimulation experiments by using an implanted electrode to assist in rehabilitation after severe TBI. The proposed model aims to reduce animal mortality during surgery and alleviate the negative side effects potentially caused by prolonged anesthesia drug usage. Isoflurane is applied as an anesthetic drug before stereotaxic surgery in rodents, which promotes hypothermia in the animal body. The result showed notable improvement in rodent survival after applying an active warming pad system to prevent hypothermia. Compared with the conventional stereotaxic system, the modified CCI device with a mounted 3D-printed header significantly improved performance in the surgical procedure, decreasing the total operation time by 21.7%, especially in the Bregma‒Lambda measurement. These findings indicate the tangible capability of our modified stereotaxic system, which allows surgeons to perform stereotaxic surgery faster and lowers the risk of intraoperative mortality.https://doi.org/10.1038/s41598-025-05328-yNeurostimulationStereotaxicTraumatic brain injuryControlled cortical impactNeurosurgery |
| spellingShingle | Pongsakorn Wechakarn Sumeth Klomchitcharoen Tirapoot Jatupornpoonsub Nantawachara Jirakittayakorn Chanon Puttanawarut Wanlop Likitsuntonwong Khuanjit Chaimongkolnukul Yodchanan Wongsawat Modified stereotactic neurosurgery techniques for rodent surgery enhance survival and reduce surgery time in a severe traumatic brain injury model Scientific Reports Neurostimulation Stereotaxic Traumatic brain injury Controlled cortical impact Neurosurgery |
| title | Modified stereotactic neurosurgery techniques for rodent surgery enhance survival and reduce surgery time in a severe traumatic brain injury model |
| title_full | Modified stereotactic neurosurgery techniques for rodent surgery enhance survival and reduce surgery time in a severe traumatic brain injury model |
| title_fullStr | Modified stereotactic neurosurgery techniques for rodent surgery enhance survival and reduce surgery time in a severe traumatic brain injury model |
| title_full_unstemmed | Modified stereotactic neurosurgery techniques for rodent surgery enhance survival and reduce surgery time in a severe traumatic brain injury model |
| title_short | Modified stereotactic neurosurgery techniques for rodent surgery enhance survival and reduce surgery time in a severe traumatic brain injury model |
| title_sort | modified stereotactic neurosurgery techniques for rodent surgery enhance survival and reduce surgery time in a severe traumatic brain injury model |
| topic | Neurostimulation Stereotaxic Traumatic brain injury Controlled cortical impact Neurosurgery |
| url | https://doi.org/10.1038/s41598-025-05328-y |
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