Fine Mapping of <i>Bush</i> Gene and Development of Molecular Marker for Bush Type in Pumpkin (<i>Cucurbita maxima</i> Duch.)
The bush trait is an important agronomic trait with great value for pumpkin crops. In this study, a bush-type line, CS82, identified in pumpkin (<i>Cucurbita maxima</i>) showed no obvious main vine, and all petioles clustered at the extremely shortened stem with limited internodes. The m...
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2024-12-01
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| author | Chun Li Zhen He Yifan Li Yanhong Lan Fangyi Gong Peng Cai Nan Yang Yan Liu Feng Xia Genyun Liang Xiaojun Liu |
| author_facet | Chun Li Zhen He Yifan Li Yanhong Lan Fangyi Gong Peng Cai Nan Yang Yan Liu Feng Xia Genyun Liang Xiaojun Liu |
| author_sort | Chun Li |
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| description | The bush trait is an important agronomic trait with great value for pumpkin crops. In this study, a bush-type line, CS82, identified in pumpkin (<i>Cucurbita maxima</i>) showed no obvious main vine, and all petioles clustered at the extremely shortened stem with limited internodes. The microscopy analysis revealed that the bush-type phenotype may be due to the degeneration of the shoot apex. Genetic analysis showed that the bush-type phenotype is controlled by a single dominant nuclear gene. Exogenous gibberellin treatment could not recover the bush-type phenotype to the wild type, indicating that the bush-type phenotype is not due to the mutation of gibberellin biosynthesis genes. The BSA-seq analysis preliminarily mapped the <i>Bush</i> gene to Chr.15 of the pumpkin genome. Further fine mapping limited the <i>Bush</i> gene to a physical distance of 95.65 kb with 19 genes. Based on the gene function and the 63 bp deletion, <i>CmaCh15G011490</i>, encoding an axial regulator YABBY 5-like protein, was selected as the candidate gene for the <i>Bush</i> gene. A quick and efficient method was developed for bush-type phenotype identification, which is useful for bush-type variety breeding in pumpkin. |
| format | Article |
| id | doaj-art-df3df6f5bf494721bd013efa74a67946 |
| institution | OA Journals |
| issn | 2073-4395 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
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| series | Agronomy |
| spelling | doaj-art-df3df6f5bf494721bd013efa74a679462025-08-20T02:00:55ZengMDPI AGAgronomy2073-43952024-12-011412296710.3390/agronomy14122967Fine Mapping of <i>Bush</i> Gene and Development of Molecular Marker for Bush Type in Pumpkin (<i>Cucurbita maxima</i> Duch.)Chun Li0Zhen He1Yifan Li2Yanhong Lan3Fangyi Gong4Peng Cai5Nan Yang6Yan Liu7Feng Xia8Genyun Liang9Xiaojun Liu10Horticulutre Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, ChinaHorticulutre Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, ChinaHorticulutre Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, ChinaHorticulutre Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, ChinaHorticulutre Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, ChinaHorticulutre Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, ChinaHorticulutre Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, ChinaHorticulutre Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, ChinaHorticulutre Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, ChinaHorticulutre Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, ChinaHorticulutre Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, ChinaThe bush trait is an important agronomic trait with great value for pumpkin crops. In this study, a bush-type line, CS82, identified in pumpkin (<i>Cucurbita maxima</i>) showed no obvious main vine, and all petioles clustered at the extremely shortened stem with limited internodes. The microscopy analysis revealed that the bush-type phenotype may be due to the degeneration of the shoot apex. Genetic analysis showed that the bush-type phenotype is controlled by a single dominant nuclear gene. Exogenous gibberellin treatment could not recover the bush-type phenotype to the wild type, indicating that the bush-type phenotype is not due to the mutation of gibberellin biosynthesis genes. The BSA-seq analysis preliminarily mapped the <i>Bush</i> gene to Chr.15 of the pumpkin genome. Further fine mapping limited the <i>Bush</i> gene to a physical distance of 95.65 kb with 19 genes. Based on the gene function and the 63 bp deletion, <i>CmaCh15G011490</i>, encoding an axial regulator YABBY 5-like protein, was selected as the candidate gene for the <i>Bush</i> gene. A quick and efficient method was developed for bush-type phenotype identification, which is useful for bush-type variety breeding in pumpkin.https://www.mdpi.com/2073-4395/14/12/2967BSA-seqbush trait<i>Cucurbita maxima</i>plant architecture |
| spellingShingle | Chun Li Zhen He Yifan Li Yanhong Lan Fangyi Gong Peng Cai Nan Yang Yan Liu Feng Xia Genyun Liang Xiaojun Liu Fine Mapping of <i>Bush</i> Gene and Development of Molecular Marker for Bush Type in Pumpkin (<i>Cucurbita maxima</i> Duch.) Agronomy BSA-seq bush trait <i>Cucurbita maxima</i> plant architecture |
| title | Fine Mapping of <i>Bush</i> Gene and Development of Molecular Marker for Bush Type in Pumpkin (<i>Cucurbita maxima</i> Duch.) |
| title_full | Fine Mapping of <i>Bush</i> Gene and Development of Molecular Marker for Bush Type in Pumpkin (<i>Cucurbita maxima</i> Duch.) |
| title_fullStr | Fine Mapping of <i>Bush</i> Gene and Development of Molecular Marker for Bush Type in Pumpkin (<i>Cucurbita maxima</i> Duch.) |
| title_full_unstemmed | Fine Mapping of <i>Bush</i> Gene and Development of Molecular Marker for Bush Type in Pumpkin (<i>Cucurbita maxima</i> Duch.) |
| title_short | Fine Mapping of <i>Bush</i> Gene and Development of Molecular Marker for Bush Type in Pumpkin (<i>Cucurbita maxima</i> Duch.) |
| title_sort | fine mapping of i bush i gene and development of molecular marker for bush type in pumpkin i cucurbita maxima i duch |
| topic | BSA-seq bush trait <i>Cucurbita maxima</i> plant architecture |
| url | https://www.mdpi.com/2073-4395/14/12/2967 |
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