Fine Mapping of <i>Bush</i> Gene and Development of Molecular Marker for Bush Type in Pumpkin (<i>Cucurbita maxima</i> Duch.)
The bush trait is an important agronomic trait with great value for pumpkin crops. In this study, a bush-type line, CS82, identified in pumpkin (<i>Cucurbita maxima</i>) showed no obvious main vine, and all petioles clustered at the extremely shortened stem with limited internodes. The m...
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| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-12-01
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| Series: | Agronomy |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4395/14/12/2967 |
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| Summary: | The bush trait is an important agronomic trait with great value for pumpkin crops. In this study, a bush-type line, CS82, identified in pumpkin (<i>Cucurbita maxima</i>) showed no obvious main vine, and all petioles clustered at the extremely shortened stem with limited internodes. The microscopy analysis revealed that the bush-type phenotype may be due to the degeneration of the shoot apex. Genetic analysis showed that the bush-type phenotype is controlled by a single dominant nuclear gene. Exogenous gibberellin treatment could not recover the bush-type phenotype to the wild type, indicating that the bush-type phenotype is not due to the mutation of gibberellin biosynthesis genes. The BSA-seq analysis preliminarily mapped the <i>Bush</i> gene to Chr.15 of the pumpkin genome. Further fine mapping limited the <i>Bush</i> gene to a physical distance of 95.65 kb with 19 genes. Based on the gene function and the 63 bp deletion, <i>CmaCh15G011490</i>, encoding an axial regulator YABBY 5-like protein, was selected as the candidate gene for the <i>Bush</i> gene. A quick and efficient method was developed for bush-type phenotype identification, which is useful for bush-type variety breeding in pumpkin. |
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| ISSN: | 2073-4395 |