Myo-inositol improves developmental competence and reduces oxidative stress in porcine parthenogenetic embryos
ObjectiveMyo-inositol (Myo-Ins), the most abundant form of inositol, is an antioxidant and plays a crucial role in the development and reproduction of mammals and humans. However, information elucidating the role of Myo-Ins in porcine embryonic development after parthenogenetic activation (PA) is st...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2024-12-01
|
| Series: | Frontiers in Veterinary Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2024.1475329/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850260657537024000 |
|---|---|
| author | Ali Jawad Ali Jawad Dongjin Oh Dongjin Oh Hyerin Choi Hyerin Choi Mirae Kim Mirae Kim Jaehyung Ham Jaehyung Ham Byoung Chol Oh Joohyeong Lee Sang-Hwan Hyun Sang-Hwan Hyun Sang-Hwan Hyun Sang-Hwan Hyun |
| author_facet | Ali Jawad Ali Jawad Dongjin Oh Dongjin Oh Hyerin Choi Hyerin Choi Mirae Kim Mirae Kim Jaehyung Ham Jaehyung Ham Byoung Chol Oh Joohyeong Lee Sang-Hwan Hyun Sang-Hwan Hyun Sang-Hwan Hyun Sang-Hwan Hyun |
| author_sort | Ali Jawad |
| collection | DOAJ |
| description | ObjectiveMyo-inositol (Myo-Ins), the most abundant form of inositol, is an antioxidant and plays a crucial role in the development and reproduction of mammals and humans. However, information elucidating the role of Myo-Ins in porcine embryonic development after parthenogenetic activation (PA) is still lacking. Therefore, we investigated the effect of Myo-Ins on porcine embryos and its underlying mechanisms.MethodsIn this study, various concentrations of Myo-Ins (0, 5, 10, and 20 mM) were added to the porcine zygotic medium (PZM3) during the in vitro culture (IVC) of porcine embryos. Several characteristics were evaluated, including cleavage rate, blastocyst formation rate, intracellular glutathione (GSH) and reactive oxygen species (ROS) levels in 4–5 cell stage embryos, total cell number, apoptotic rate in blastocysts, mitochondrial membrane potential (MMP), mitochondrial quantity, mitochondrial stress in the blastocysts, and gene expression for antioxidant and mitochondrial function markers. Additionally, the immunofluorescence of HO-1 was assessed.ResultsThe results showed that Myo-Ins at concentrations of 10 and 20 mM significantly increased the blastocyst formation rate compared to the control group. Embryos supplemented with 20 mM Myo-Ins exhibited higher GSH levels and lower ROS levels than those in the control group. Myo-Ins supplementation also decreased the rate of apoptosis and the apoptotic index in the treatment groups. Additionally, embryos supplemented with 20 mM Myo-Ins showed increased mitochondrial membrane potential (MMP), greater mitochondrial quantity, and reduced oxidative stress in the mitochondria. Interestingly, the expression levels of genes related to mitochondrial function and the nuclear erythroid factor 2-related factor (NRF2) pathway were elevated in the Myo-Ins treated groups. Furthermore, immunofluorescence results indicated that 20 mM Myo-Ins significantly increased HO-1 expression in blastocysts compared to the control group.ConclusionIn conclusion, 20 mM Myo-Ins supplementation enhanced blastocyst development and improved mitochondrial function by regulating apoptosis, reducing oxidative stress, and activating the NRF2 pathway. |
| format | Article |
| id | doaj-art-df2ba582bf21438daa5d6ce858e3a154 |
| institution | OA Journals |
| issn | 2297-1769 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj-art-df2ba582bf21438daa5d6ce858e3a1542025-08-20T01:55:34ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692024-12-011110.3389/fvets.2024.14753291475329Myo-inositol improves developmental competence and reduces oxidative stress in porcine parthenogenetic embryosAli Jawad0Ali Jawad1Dongjin Oh2Dongjin Oh3Hyerin Choi4Hyerin Choi5Mirae Kim6Mirae Kim7Jaehyung Ham8Jaehyung Ham9Byoung Chol Oh10Joohyeong Lee11Sang-Hwan Hyun12Sang-Hwan Hyun13Sang-Hwan Hyun14Sang-Hwan Hyun15Veterinary Medical Center and College of Veterinary Medicine, Laboratory of Veterinary Embryology and Biotechnology (VETEMBIO), Chungbuk National University, Cheongju, Republic of KoreaInstitute of Stem Cell and Regenerative Medicine (ISCRM), Chungbuk National University, Cheongju, Republic of KoreaVeterinary Medical Center and College of Veterinary Medicine, Laboratory of Veterinary Embryology and Biotechnology (VETEMBIO), Chungbuk National University, Cheongju, Republic of KoreaInstitute of Stem Cell and Regenerative Medicine (ISCRM), Chungbuk National University, Cheongju, Republic of KoreaVeterinary Medical Center and College of Veterinary Medicine, Laboratory of Veterinary Embryology and Biotechnology (VETEMBIO), Chungbuk National University, Cheongju, Republic of KoreaInstitute of Stem Cell and Regenerative Medicine (ISCRM), Chungbuk National University, Cheongju, Republic of KoreaVeterinary Medical Center and College of Veterinary Medicine, Laboratory of Veterinary Embryology and Biotechnology (VETEMBIO), Chungbuk National University, Cheongju, Republic of KoreaInstitute of Stem Cell and Regenerative Medicine (ISCRM), Chungbuk National University, Cheongju, Republic of KoreaVeterinary Medical Center and College of Veterinary Medicine, Laboratory of Veterinary Embryology and Biotechnology (VETEMBIO), Chungbuk National University, Cheongju, Republic of KoreaInstitute of Stem Cell and Regenerative Medicine (ISCRM), Chungbuk National University, Cheongju, Republic of KoreaDepartment of Plastic and Reconstructive Surgery, Johns Hopkins University School of Medicine, Baltimore, MD, United StatesDepartment of Companion Animal Industry, College of Healthcare and Biotechnology, Semyung University, Jecheon, Republic of KoreaVeterinary Medical Center and College of Veterinary Medicine, Laboratory of Veterinary Embryology and Biotechnology (VETEMBIO), Chungbuk National University, Cheongju, Republic of KoreaInstitute of Stem Cell and Regenerative Medicine (ISCRM), Chungbuk National University, Cheongju, Republic of KoreaVet-ICT Convergence Education and Research Center (VICERC), Chungbuk National University, Cheongju, Republic of KoreaChungbuk National University Hospital, Cheongju, Republic of KoreaObjectiveMyo-inositol (Myo-Ins), the most abundant form of inositol, is an antioxidant and plays a crucial role in the development and reproduction of mammals and humans. However, information elucidating the role of Myo-Ins in porcine embryonic development after parthenogenetic activation (PA) is still lacking. Therefore, we investigated the effect of Myo-Ins on porcine embryos and its underlying mechanisms.MethodsIn this study, various concentrations of Myo-Ins (0, 5, 10, and 20 mM) were added to the porcine zygotic medium (PZM3) during the in vitro culture (IVC) of porcine embryos. Several characteristics were evaluated, including cleavage rate, blastocyst formation rate, intracellular glutathione (GSH) and reactive oxygen species (ROS) levels in 4–5 cell stage embryos, total cell number, apoptotic rate in blastocysts, mitochondrial membrane potential (MMP), mitochondrial quantity, mitochondrial stress in the blastocysts, and gene expression for antioxidant and mitochondrial function markers. Additionally, the immunofluorescence of HO-1 was assessed.ResultsThe results showed that Myo-Ins at concentrations of 10 and 20 mM significantly increased the blastocyst formation rate compared to the control group. Embryos supplemented with 20 mM Myo-Ins exhibited higher GSH levels and lower ROS levels than those in the control group. Myo-Ins supplementation also decreased the rate of apoptosis and the apoptotic index in the treatment groups. Additionally, embryos supplemented with 20 mM Myo-Ins showed increased mitochondrial membrane potential (MMP), greater mitochondrial quantity, and reduced oxidative stress in the mitochondria. Interestingly, the expression levels of genes related to mitochondrial function and the nuclear erythroid factor 2-related factor (NRF2) pathway were elevated in the Myo-Ins treated groups. Furthermore, immunofluorescence results indicated that 20 mM Myo-Ins significantly increased HO-1 expression in blastocysts compared to the control group.ConclusionIn conclusion, 20 mM Myo-Ins supplementation enhanced blastocyst development and improved mitochondrial function by regulating apoptosis, reducing oxidative stress, and activating the NRF2 pathway.https://www.frontiersin.org/articles/10.3389/fvets.2024.1475329/fullmyo-inositolembryosmitochondriaparthenogenesisoxidative stress |
| spellingShingle | Ali Jawad Ali Jawad Dongjin Oh Dongjin Oh Hyerin Choi Hyerin Choi Mirae Kim Mirae Kim Jaehyung Ham Jaehyung Ham Byoung Chol Oh Joohyeong Lee Sang-Hwan Hyun Sang-Hwan Hyun Sang-Hwan Hyun Sang-Hwan Hyun Myo-inositol improves developmental competence and reduces oxidative stress in porcine parthenogenetic embryos Frontiers in Veterinary Science myo-inositol embryos mitochondria parthenogenesis oxidative stress |
| title | Myo-inositol improves developmental competence and reduces oxidative stress in porcine parthenogenetic embryos |
| title_full | Myo-inositol improves developmental competence and reduces oxidative stress in porcine parthenogenetic embryos |
| title_fullStr | Myo-inositol improves developmental competence and reduces oxidative stress in porcine parthenogenetic embryos |
| title_full_unstemmed | Myo-inositol improves developmental competence and reduces oxidative stress in porcine parthenogenetic embryos |
| title_short | Myo-inositol improves developmental competence and reduces oxidative stress in porcine parthenogenetic embryos |
| title_sort | myo inositol improves developmental competence and reduces oxidative stress in porcine parthenogenetic embryos |
| topic | myo-inositol embryos mitochondria parthenogenesis oxidative stress |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2024.1475329/full |
| work_keys_str_mv | AT alijawad myoinositolimprovesdevelopmentalcompetenceandreducesoxidativestressinporcineparthenogeneticembryos AT alijawad myoinositolimprovesdevelopmentalcompetenceandreducesoxidativestressinporcineparthenogeneticembryos AT dongjinoh myoinositolimprovesdevelopmentalcompetenceandreducesoxidativestressinporcineparthenogeneticembryos AT dongjinoh myoinositolimprovesdevelopmentalcompetenceandreducesoxidativestressinporcineparthenogeneticembryos AT hyerinchoi myoinositolimprovesdevelopmentalcompetenceandreducesoxidativestressinporcineparthenogeneticembryos AT hyerinchoi myoinositolimprovesdevelopmentalcompetenceandreducesoxidativestressinporcineparthenogeneticembryos AT miraekim myoinositolimprovesdevelopmentalcompetenceandreducesoxidativestressinporcineparthenogeneticembryos AT miraekim myoinositolimprovesdevelopmentalcompetenceandreducesoxidativestressinporcineparthenogeneticembryos AT jaehyungham myoinositolimprovesdevelopmentalcompetenceandreducesoxidativestressinporcineparthenogeneticembryos AT jaehyungham myoinositolimprovesdevelopmentalcompetenceandreducesoxidativestressinporcineparthenogeneticembryos AT byoungcholoh myoinositolimprovesdevelopmentalcompetenceandreducesoxidativestressinporcineparthenogeneticembryos AT joohyeonglee myoinositolimprovesdevelopmentalcompetenceandreducesoxidativestressinporcineparthenogeneticembryos AT sanghwanhyun myoinositolimprovesdevelopmentalcompetenceandreducesoxidativestressinporcineparthenogeneticembryos AT sanghwanhyun myoinositolimprovesdevelopmentalcompetenceandreducesoxidativestressinporcineparthenogeneticembryos AT sanghwanhyun myoinositolimprovesdevelopmentalcompetenceandreducesoxidativestressinporcineparthenogeneticembryos AT sanghwanhyun myoinositolimprovesdevelopmentalcompetenceandreducesoxidativestressinporcineparthenogeneticembryos |