Determination of Water Permeability Parameters and Optimal Freezing Rates of Human Red Blood Cells by Differential Scanning Calorimeter
Optimization of human red blood cells (RBCs) cryopreservation protocols requires an understanding of the water permeability characteristics. Volume shrinkage during freezing of RBCs can be obtained by using a shape-independent differential scanning calorimeter (DSC) technique. By ?tting the water tr...
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| Format: | Article |
| Language: | zho |
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Journal of Refrigeration Magazines Agency Co., Ltd.
2012-01-01
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| Series: | Zhileng xuebao |
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| Online Access: | http://www.zhilengxuebao.com/thesisDetails#10.3969/j.issn.0253-4339.2012.04.012 |
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| author | Zhou Xinli Yuan Ji Wang Haisong Li Weijie |
| author_facet | Zhou Xinli Yuan Ji Wang Haisong Li Weijie |
| author_sort | Zhou Xinli |
| collection | DOAJ |
| description | Optimization of human red blood cells (RBCs) cryopreservation protocols requires an understanding of the water permeability characteristics. Volume shrinkage during freezing of RBCs can be obtained by using a shape-independent differential scanning calorimeter (DSC) technique. By ?tting the water transport model to the experimentally obtained DSC data, the best-?t membrane permeability parameters (reference membrane permeability to water and the activation energy) in normal saline, 20 % dimethyl sulfoxide (Me2SO), 10% glycerol and 20% glycerol were determined. These parameters were then used to simulate water transport of RBCs at constant cooling rates between 0.5~50℃/min. The simulation results showed “optimal cooling rate” for erythrocytes were 5.2℃/min, 5.5℃/min and 4.6℃/min in 20 % Me2SO, 10% and 20% glycerol respectively. |
| format | Article |
| id | doaj-art-df2ba44279534dbb9e0ab571acbf62a3 |
| institution | DOAJ |
| issn | 0253-4339 |
| language | zho |
| publishDate | 2012-01-01 |
| publisher | Journal of Refrigeration Magazines Agency Co., Ltd. |
| record_format | Article |
| series | Zhileng xuebao |
| spelling | doaj-art-df2ba44279534dbb9e0ab571acbf62a32025-08-20T03:00:13ZzhoJournal of Refrigeration Magazines Agency Co., Ltd.Zhileng xuebao0253-43392012-01-013367075392Determination of Water Permeability Parameters and Optimal Freezing Rates of Human Red Blood Cells by Differential Scanning CalorimeterZhou XinliYuan JiWang HaisongLi WeijieOptimization of human red blood cells (RBCs) cryopreservation protocols requires an understanding of the water permeability characteristics. Volume shrinkage during freezing of RBCs can be obtained by using a shape-independent differential scanning calorimeter (DSC) technique. By ?tting the water transport model to the experimentally obtained DSC data, the best-?t membrane permeability parameters (reference membrane permeability to water and the activation energy) in normal saline, 20 % dimethyl sulfoxide (Me2SO), 10% glycerol and 20% glycerol were determined. These parameters were then used to simulate water transport of RBCs at constant cooling rates between 0.5~50℃/min. The simulation results showed “optimal cooling rate” for erythrocytes were 5.2℃/min, 5.5℃/min and 4.6℃/min in 20 % Me2SO, 10% and 20% glycerol respectively.http://www.zhilengxuebao.com/thesisDetails#10.3969/j.issn.0253-4339.2012.04.012pyrologypermeability parametersoptimal cooling rateDSCred blood cell |
| spellingShingle | Zhou Xinli Yuan Ji Wang Haisong Li Weijie Determination of Water Permeability Parameters and Optimal Freezing Rates of Human Red Blood Cells by Differential Scanning Calorimeter Zhileng xuebao pyrology permeability parameters optimal cooling rate DSC red blood cell |
| title | Determination of Water Permeability Parameters and Optimal Freezing Rates of Human Red Blood Cells by Differential Scanning Calorimeter |
| title_full | Determination of Water Permeability Parameters and Optimal Freezing Rates of Human Red Blood Cells by Differential Scanning Calorimeter |
| title_fullStr | Determination of Water Permeability Parameters and Optimal Freezing Rates of Human Red Blood Cells by Differential Scanning Calorimeter |
| title_full_unstemmed | Determination of Water Permeability Parameters and Optimal Freezing Rates of Human Red Blood Cells by Differential Scanning Calorimeter |
| title_short | Determination of Water Permeability Parameters and Optimal Freezing Rates of Human Red Blood Cells by Differential Scanning Calorimeter |
| title_sort | determination of water permeability parameters and optimal freezing rates of human red blood cells by differential scanning calorimeter |
| topic | pyrology permeability parameters optimal cooling rate DSC red blood cell |
| url | http://www.zhilengxuebao.com/thesisDetails#10.3969/j.issn.0253-4339.2012.04.012 |
| work_keys_str_mv | AT zhouxinli determinationofwaterpermeabilityparametersandoptimalfreezingratesofhumanredbloodcellsbydifferentialscanningcalorimeter AT yuanji determinationofwaterpermeabilityparametersandoptimalfreezingratesofhumanredbloodcellsbydifferentialscanningcalorimeter AT wanghaisong determinationofwaterpermeabilityparametersandoptimalfreezingratesofhumanredbloodcellsbydifferentialscanningcalorimeter AT liweijie determinationofwaterpermeabilityparametersandoptimalfreezingratesofhumanredbloodcellsbydifferentialscanningcalorimeter |