Determination of Water Permeability Parameters and Optimal Freezing Rates of Human Red Blood Cells by Differential Scanning Calorimeter
Optimization of human red blood cells (RBCs) cryopreservation protocols requires an understanding of the water permeability characteristics. Volume shrinkage during freezing of RBCs can be obtained by using a shape-independent differential scanning calorimeter (DSC) technique. By ?tting the water tr...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | zho |
| Published: |
Journal of Refrigeration Magazines Agency Co., Ltd.
2012-01-01
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| Series: | Zhileng xuebao |
| Subjects: | |
| Online Access: | http://www.zhilengxuebao.com/thesisDetails#10.3969/j.issn.0253-4339.2012.04.012 |
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| Summary: | Optimization of human red blood cells (RBCs) cryopreservation protocols requires an understanding of the water permeability characteristics. Volume shrinkage during freezing of RBCs can be obtained by using a shape-independent differential scanning calorimeter (DSC) technique. By ?tting the water transport model to the experimentally obtained DSC data, the best-?t membrane permeability parameters (reference membrane permeability to water and the activation energy) in normal saline, 20 % dimethyl sulfoxide (Me2SO), 10% glycerol and 20% glycerol were determined. These parameters were then used to simulate water transport of RBCs at constant cooling rates between 0.5~50℃/min. The simulation results showed “optimal cooling rate” for erythrocytes were 5.2℃/min, 5.5℃/min and 4.6℃/min in 20 % Me2SO, 10% and 20% glycerol respectively. |
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| ISSN: | 0253-4339 |