CAPG enhances adipogenesis and inflammatory cytokine expression in adipocytes
The expression of CAPG (capping actin protein, gelsolin-like) is upregulated in visceral white adipose tissue of high-fat diet -fed mice; however, its impact on adipocyte functionality remains unclear. We observed upregulated CAPG expression in the epididymal adipose tissue of high-fat diet-fed mice...
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| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2025-12-01
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| Series: | Adipocyte |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/21623945.2025.2549456 |
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| author | Luyao Zhang Botao Sang Sainan Li Ying Li Dachuan Guo Qi-Nan Ma Xiangfei Liu Xiaoshuo Li Beidong Chen Deping Liu |
| author_facet | Luyao Zhang Botao Sang Sainan Li Ying Li Dachuan Guo Qi-Nan Ma Xiangfei Liu Xiaoshuo Li Beidong Chen Deping Liu |
| author_sort | Luyao Zhang |
| collection | DOAJ |
| description | The expression of CAPG (capping actin protein, gelsolin-like) is upregulated in visceral white adipose tissue of high-fat diet -fed mice; however, its impact on adipocyte functionality remains unclear. We observed upregulated CAPG expression in the epididymal adipose tissue of high-fat diet-fed mice. To investigate the impact of CAPG on adipocyte differentiation and function, we generated a Capg knockdown 3T3-L1 cell line and induced adipogenic differentiation to mature adipocytes. Adipogenesis was assessed via Oil Red O and BODIPY staining, revealing that Capg knockdown markedly suppressed adipogenesis. Western blot analysis demonstrated that CAPG depletion reduced PPARγ expression. Additionally, Western blot analysis revealed that Capg knockdown significantly enhanced lipid utilization in adipocytes. ELISA and qPCR results further demonstrated that Capg knockdown effectively attenuated inflammatory responses in adipocytes. In conclusion, CAPG promotes adipogenesis and inflammatory responses, suggesting that targeted inhibition of CAPG may represent a potential therapeutic strategy for obesity-associated adipose tissue dysfunction. |
| format | Article |
| id | doaj-art-df2297722d89479ea7b32c41421b5beb |
| institution | Kabale University |
| issn | 2162-3945 2162-397X |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Adipocyte |
| spelling | doaj-art-df2297722d89479ea7b32c41421b5beb2025-08-26T06:23:24ZengTaylor & Francis GroupAdipocyte2162-39452162-397X2025-12-0114110.1080/21623945.2025.2549456CAPG enhances adipogenesis and inflammatory cytokine expression in adipocytesLuyao Zhang0Botao Sang1Sainan Li2Ying Li3Dachuan Guo4Qi-Nan Ma5Xiangfei Liu6Xiaoshuo Li7Beidong Chen8Deping Liu9Department of Cardiology, Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing, ChinaDepartment of Cardiology, Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine, Medical School, University of Chinese Academy of Sciences, Beijing, ChinaDepartment of Cardiology, Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing, ChinaDepartment of Cardiology, Beijing Hospital, National Center of Gerontology, Chinese Academy of Medical Sciences, Institute of Geriatric Medicine, Beijing University, Beijing, ChinaDepartment of Cardiology, Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing, ChinaDepartment of Cardiology, Beijing Hospital, National Center of Gerontology, Chinese Academy of Medical Sciences, Institute of Geriatric Medicine, Beijing University, Beijing, ChinaDepartment of Cardiology, Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing, ChinaDepartment of Cardiology, Beijing Hospital, National Center of Gerontology, Chinese Academy of Medical Sciences, Institute of Geriatric Medicine, Beijing University, Beijing, ChinaThe Key Laboratory of Geriatrics, Beijing Institute of Geriatrics, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing Hospital/National Center of Gerontology of National Health Commission, Beijing, ChinaDepartment of Cardiology, Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing, ChinaThe expression of CAPG (capping actin protein, gelsolin-like) is upregulated in visceral white adipose tissue of high-fat diet -fed mice; however, its impact on adipocyte functionality remains unclear. We observed upregulated CAPG expression in the epididymal adipose tissue of high-fat diet-fed mice. To investigate the impact of CAPG on adipocyte differentiation and function, we generated a Capg knockdown 3T3-L1 cell line and induced adipogenic differentiation to mature adipocytes. Adipogenesis was assessed via Oil Red O and BODIPY staining, revealing that Capg knockdown markedly suppressed adipogenesis. Western blot analysis demonstrated that CAPG depletion reduced PPARγ expression. Additionally, Western blot analysis revealed that Capg knockdown significantly enhanced lipid utilization in adipocytes. ELISA and qPCR results further demonstrated that Capg knockdown effectively attenuated inflammatory responses in adipocytes. In conclusion, CAPG promotes adipogenesis and inflammatory responses, suggesting that targeted inhibition of CAPG may represent a potential therapeutic strategy for obesity-associated adipose tissue dysfunction.https://www.tandfonline.com/doi/10.1080/21623945.2025.2549456CAPGadipocyteadipogenesisinflammationmetabolism |
| spellingShingle | Luyao Zhang Botao Sang Sainan Li Ying Li Dachuan Guo Qi-Nan Ma Xiangfei Liu Xiaoshuo Li Beidong Chen Deping Liu CAPG enhances adipogenesis and inflammatory cytokine expression in adipocytes Adipocyte CAPG adipocyte adipogenesis inflammation metabolism |
| title | CAPG enhances adipogenesis and inflammatory cytokine expression in adipocytes |
| title_full | CAPG enhances adipogenesis and inflammatory cytokine expression in adipocytes |
| title_fullStr | CAPG enhances adipogenesis and inflammatory cytokine expression in adipocytes |
| title_full_unstemmed | CAPG enhances adipogenesis and inflammatory cytokine expression in adipocytes |
| title_short | CAPG enhances adipogenesis and inflammatory cytokine expression in adipocytes |
| title_sort | capg enhances adipogenesis and inflammatory cytokine expression in adipocytes |
| topic | CAPG adipocyte adipogenesis inflammation metabolism |
| url | https://www.tandfonline.com/doi/10.1080/21623945.2025.2549456 |
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