Phenotypic and Genomic Insights into <i>Schleiferilactobacillus harbinensis</i> WU01, a Candidate Probiotic with Broad-Spectrum Antimicrobial Activity Against ESKAPE (<i>Enterococcus faecium</i>, <i>Staphylococcus aureus</i>, <i>Klebsiella pneumoniae</i>, <i>Acinetobacter baumannii</i>, <i>Pseudomonas aeruginosa</i>, and <i>Enterobacter</i>) Pathogens
The increasing prevalence of multidrug-resistant (MDR) pathogens, particularly ESKAPE bacteria, necessitates alternative antimicrobial strategies. Probiotics, particularly lactic acid bacteria, protect against pathogenic infections. This study aimed to characterize <i>Schleiferilactobacillus h...
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2025-03-01
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| author | Chonticha Romyasamit Komwit Surachat Nawanwat C. Pattaranggoon Pinkanok Suksabay Uttapol Permpoon Tae-Gyu Nam Phoomjai Sornsenee |
| author_facet | Chonticha Romyasamit Komwit Surachat Nawanwat C. Pattaranggoon Pinkanok Suksabay Uttapol Permpoon Tae-Gyu Nam Phoomjai Sornsenee |
| author_sort | Chonticha Romyasamit |
| collection | DOAJ |
| description | The increasing prevalence of multidrug-resistant (MDR) pathogens, particularly ESKAPE bacteria, necessitates alternative antimicrobial strategies. Probiotics, particularly lactic acid bacteria, protect against pathogenic infections. This study aimed to characterize <i>Schleiferilactobacillus harbinensis</i> WU01, isolated from fermented palm sap, and evaluate its probiotic potential and antimicrobial activity. Its probiotic characteristics were assessed based on low-pH and bile tolerance, auto-aggregation, hydrophobicity, and adhesion to Caco-2 cells. Antimicrobial activity against ESKAPE pathogens was evaluated using the agar well diffusion assay. Whole-genome sequencing (WGS) and in silico analysis were performed to identify bacteriocin-related genes, virulence factors, and antibiotic-resistance genes. WU01 exhibited a strong tolerance to gastrointestinal conditions, with high survival rates under acidic and bile-salt environments. <i>S. harbinensis</i> WU01 demonstrated significant auto-aggregation, high hydrophobicity, and strong adhesion to Caco-2 cells. Antimicrobial assays revealed inhibitory activity against MDR ESKAPE pathogens, which correlated with the presence of bacteriocin-related genes, including those homologous to Carnocin_CP52. Molecular dynamics (MDs) simulations confirmed the interaction of Carnocin_CP52 with bacterial membranes, suggesting a mechanism for pathogen disruption. WGS confirmed the absence of virulence and antimicrobial-resistance genes, confirming its safety for probiotic applications. These findings suggest that <i>S. harbinensis</i> WU01 possesses probiotic properties and antimicrobial activity against ESKAPE pathogens. The combined results highlight its potential application in functional foods and therapeutic interventions. |
| format | Article |
| id | doaj-art-df138e7de3ec464782e8563dad3ecdad |
| institution | DOAJ |
| issn | 2304-8158 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj-art-df138e7de3ec464782e8563dad3ecdad2025-08-20T03:08:55ZengMDPI AGFoods2304-81582025-03-01147116110.3390/foods14071161Phenotypic and Genomic Insights into <i>Schleiferilactobacillus harbinensis</i> WU01, a Candidate Probiotic with Broad-Spectrum Antimicrobial Activity Against ESKAPE (<i>Enterococcus faecium</i>, <i>Staphylococcus aureus</i>, <i>Klebsiella pneumoniae</i>, <i>Acinetobacter baumannii</i>, <i>Pseudomonas aeruginosa</i>, and <i>Enterobacter</i>) PathogensChonticha Romyasamit0Komwit Surachat1Nawanwat C. Pattaranggoon2Pinkanok Suksabay3Uttapol Permpoon4Tae-Gyu Nam5Phoomjai Sornsenee6Department of Medical Technology, School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat 80160, ThailandDepartment of Biomedical Sciences and Biomedical Engineering, Faculty of Medicine, Prince of Songkla University, Hat Yai, Songkhla 90110, ThailandFaculty of Medical Technology, Rangsit University, Muang Pathumthani, Pathumthani 12000, ThailandDepartment of Medical Technology, School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat 80160, ThailandDepartment of Pharmacy, Institute of Pharmaceutical Science and Technology, Hanyang University ERICA, Ansan 15588, Republic of KoreaDepartment of Pharmacy, Institute of Pharmaceutical Science and Technology, Hanyang University ERICA, Ansan 15588, Republic of KoreaDepartment of Family and Preventive Medicine, Faculty of Medicine, Prince of Songkla University, Songkhla 90110, ThailandThe increasing prevalence of multidrug-resistant (MDR) pathogens, particularly ESKAPE bacteria, necessitates alternative antimicrobial strategies. Probiotics, particularly lactic acid bacteria, protect against pathogenic infections. This study aimed to characterize <i>Schleiferilactobacillus harbinensis</i> WU01, isolated from fermented palm sap, and evaluate its probiotic potential and antimicrobial activity. Its probiotic characteristics were assessed based on low-pH and bile tolerance, auto-aggregation, hydrophobicity, and adhesion to Caco-2 cells. Antimicrobial activity against ESKAPE pathogens was evaluated using the agar well diffusion assay. Whole-genome sequencing (WGS) and in silico analysis were performed to identify bacteriocin-related genes, virulence factors, and antibiotic-resistance genes. WU01 exhibited a strong tolerance to gastrointestinal conditions, with high survival rates under acidic and bile-salt environments. <i>S. harbinensis</i> WU01 demonstrated significant auto-aggregation, high hydrophobicity, and strong adhesion to Caco-2 cells. Antimicrobial assays revealed inhibitory activity against MDR ESKAPE pathogens, which correlated with the presence of bacteriocin-related genes, including those homologous to Carnocin_CP52. Molecular dynamics (MDs) simulations confirmed the interaction of Carnocin_CP52 with bacterial membranes, suggesting a mechanism for pathogen disruption. WGS confirmed the absence of virulence and antimicrobial-resistance genes, confirming its safety for probiotic applications. These findings suggest that <i>S. harbinensis</i> WU01 possesses probiotic properties and antimicrobial activity against ESKAPE pathogens. The combined results highlight its potential application in functional foods and therapeutic interventions.https://www.mdpi.com/2304-8158/14/7/1161<i>Schleiferilactobacillus harbinensis</i>probioticsantimicrobial activityESKAPE pathogenswhole-genome sequencing |
| spellingShingle | Chonticha Romyasamit Komwit Surachat Nawanwat C. Pattaranggoon Pinkanok Suksabay Uttapol Permpoon Tae-Gyu Nam Phoomjai Sornsenee Phenotypic and Genomic Insights into <i>Schleiferilactobacillus harbinensis</i> WU01, a Candidate Probiotic with Broad-Spectrum Antimicrobial Activity Against ESKAPE (<i>Enterococcus faecium</i>, <i>Staphylococcus aureus</i>, <i>Klebsiella pneumoniae</i>, <i>Acinetobacter baumannii</i>, <i>Pseudomonas aeruginosa</i>, and <i>Enterobacter</i>) Pathogens Foods <i>Schleiferilactobacillus harbinensis</i> probiotics antimicrobial activity ESKAPE pathogens whole-genome sequencing |
| title | Phenotypic and Genomic Insights into <i>Schleiferilactobacillus harbinensis</i> WU01, a Candidate Probiotic with Broad-Spectrum Antimicrobial Activity Against ESKAPE (<i>Enterococcus faecium</i>, <i>Staphylococcus aureus</i>, <i>Klebsiella pneumoniae</i>, <i>Acinetobacter baumannii</i>, <i>Pseudomonas aeruginosa</i>, and <i>Enterobacter</i>) Pathogens |
| title_full | Phenotypic and Genomic Insights into <i>Schleiferilactobacillus harbinensis</i> WU01, a Candidate Probiotic with Broad-Spectrum Antimicrobial Activity Against ESKAPE (<i>Enterococcus faecium</i>, <i>Staphylococcus aureus</i>, <i>Klebsiella pneumoniae</i>, <i>Acinetobacter baumannii</i>, <i>Pseudomonas aeruginosa</i>, and <i>Enterobacter</i>) Pathogens |
| title_fullStr | Phenotypic and Genomic Insights into <i>Schleiferilactobacillus harbinensis</i> WU01, a Candidate Probiotic with Broad-Spectrum Antimicrobial Activity Against ESKAPE (<i>Enterococcus faecium</i>, <i>Staphylococcus aureus</i>, <i>Klebsiella pneumoniae</i>, <i>Acinetobacter baumannii</i>, <i>Pseudomonas aeruginosa</i>, and <i>Enterobacter</i>) Pathogens |
| title_full_unstemmed | Phenotypic and Genomic Insights into <i>Schleiferilactobacillus harbinensis</i> WU01, a Candidate Probiotic with Broad-Spectrum Antimicrobial Activity Against ESKAPE (<i>Enterococcus faecium</i>, <i>Staphylococcus aureus</i>, <i>Klebsiella pneumoniae</i>, <i>Acinetobacter baumannii</i>, <i>Pseudomonas aeruginosa</i>, and <i>Enterobacter</i>) Pathogens |
| title_short | Phenotypic and Genomic Insights into <i>Schleiferilactobacillus harbinensis</i> WU01, a Candidate Probiotic with Broad-Spectrum Antimicrobial Activity Against ESKAPE (<i>Enterococcus faecium</i>, <i>Staphylococcus aureus</i>, <i>Klebsiella pneumoniae</i>, <i>Acinetobacter baumannii</i>, <i>Pseudomonas aeruginosa</i>, and <i>Enterobacter</i>) Pathogens |
| title_sort | phenotypic and genomic insights into i schleiferilactobacillus harbinensis i wu01 a candidate probiotic with broad spectrum antimicrobial activity against eskape i enterococcus faecium i i staphylococcus aureus i i klebsiella pneumoniae i i acinetobacter baumannii i i pseudomonas aeruginosa i and i enterobacter i pathogens |
| topic | <i>Schleiferilactobacillus harbinensis</i> probiotics antimicrobial activity ESKAPE pathogens whole-genome sequencing |
| url | https://www.mdpi.com/2304-8158/14/7/1161 |
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