Synergistic effects of AgNPs and zileuton on PCOS via ferroptosis and inflammation mitigation

Synergistic effects of AgNPs and zileuton on PCOS via ferroptosis and inflammation mitigation

Background: The most prevalent endocrine disorder affecting women is PCOS. Programmed death of ovarian cells has yet to be elucidated. Ferroptosis is a kind of iron-dependent necrosis featured by significantly Fe+2-dependent lipid peroxidation. The ongoing study aimed to reinforce fertility by combi...

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Main Authors: Amira K. Eltokhy, Rehab Ahmed Ahmed El-shaer, Omnia Safwat El-Deeb, Eman E. Farghal, Rowida Raafat Ibrahim, Rasha Elesawy, Marwa Mahmoud Awad, Radwa Ismail, Shaimaa M. Motawea, Doaa Shatat, Yasser Mostafa Hafez, Hend Ahmed El hanafy, Marwa Mohamed Atef
Format: Article
Language:English
Published: Taylor & Francis Group 2025-12-01
Series:Redox Report
Subjects:
Ferroptosis
Panx1
TLR4
COX-2
VEGF
AgNPs
Online Access:https://www.tandfonline.com/doi/10.1080/13510002.2024.2445398
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Summary:Background: The most prevalent endocrine disorder affecting women is PCOS. Programmed death of ovarian cells has yet to be elucidated. Ferroptosis is a kind of iron-dependent necrosis featured by significantly Fe+2-dependent lipid peroxidation. The ongoing study aimed to reinforce fertility by combining therapy with AgNPs and (Zileuton) in PCOS rats’ model.Methods: The study included 75 adult female rats divided into 5 groups; control, PCOS, PCOS treated with AgNPs, PCOS treated with Zileuton, and PCOS group treated with AgNPs and Zileuton. The study investigated the anti-ferroptotic, anti-inflammatory, antioxidant, antiapoptotic, histopathological and immunohistochemical examinations of COX-2 and VEGF.Results: The combination of AgNPs and Zileuton showed significant reduction of inflammatory mediators (IL-6, TNF-α, NFk-B) compared with diseased group (P-value < 0.05), regression of ferroptosis marks (Panx1 and TLR4 expression, Fe+2 levels) compared with diseased group (P-value < 0.05), depression of apoptotic marker caspase 3 level compared with diseased animals (P-value < 0.05), depression of MDA level, elevation of HO-1, GPx4 activity, and reduction of Cox2 and VEGF as compared with the diseased, AgNPs or zileuton-treated groups (P-value < 0.05).Conclusion: The study showed that the combination of AgNPs and zileuton guards against, inflammation, apoptosis, and ferroptosis in PCO.
ISSN:1351-0002
1743-2928

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