Morphological and molecular identification for two new wood-inhabiting species of Botryobasidium (Basidiomycota) from China

The wood-inhabiting fungi refer to large basidiomycetes that grow on various woody materials and are distributed in various forest ecosystems, some of which have important economic value. In the present study, two new resupinate, adnate, wood-inhabiting fungal taxa, Botryobasidium latihyphum and B....

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Main Authors: Xin Li, Xin Zhang, Yi-Fei Sun, Zhen-Hao Li, An-Hong Zhu, Ying-Da Wu
Format: Article
Language:English
Published: Pensoft Publishers 2025-04-01
Series:MycoKeys
Online Access:https://mycokeys.pensoft.net/article/143594/download/pdf/
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author Xin Li
Xin Zhang
Yi-Fei Sun
Zhen-Hao Li
An-Hong Zhu
Ying-Da Wu
author_facet Xin Li
Xin Zhang
Yi-Fei Sun
Zhen-Hao Li
An-Hong Zhu
Ying-Da Wu
author_sort Xin Li
collection DOAJ
description The wood-inhabiting fungi refer to large basidiomycetes that grow on various woody materials and are distributed in various forest ecosystems, some of which have important economic value. In the present study, two new resupinate, adnate, wood-inhabiting fungal taxa, Botryobasidium latihyphum and B. zhejiangensis, are introduced based on morphological and molecular characteristics. A molecular phylogenetic study based on sequence data from the internal transcribed spacers (ITS) and the large subunit (nLSU) regions supported the two new species in the genus Botryobasidium. Maximum likelihood (ML), maximum parsimony (MP), and Bayesian inference (BIBI) were employed to perform phylogenetic analyses of these datasets. The new species B. latihyphum is characterized by its cream hymenial surface when fresh, olivaceous buff when dry, a monomitic hyphal system with clamp connections, the presence of clavate to tubular cystidia, basidia with six sterigmata, and broadly oval basidiospores measuring 7.9–10.2 × 3.2–4.3 μm. Botryobasidium zhejiangensis sp. nov. is characterized by its white to buff-yellow hymenial surface when fresh, cream when dry, a monomitic hyphal system with clamp connections, lacking cystidia, basidia with six sterigmata, and broadly navicular basidiospores measuring 7.9–9.2 × 2.6–3.4 μm. The phylogenetic result inferred from ITS + nLSU sequence data revealed that B. latihyphum is closely related to B. vagum, B. laeve, B. subincanum, and B. incanum, while B. zhejiangensis is closely related to B. leptocystidiatum, B. subcoronatum, B. xizangense, and B. intertextum.
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issn 1314-4049
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spelling doaj-art-df0a8b28688e4605a3e158d807db32e32025-08-20T03:06:25ZengPensoft PublishersMycoKeys1314-40492025-04-01116738910.3897/mycokeys.116.143594143594Morphological and molecular identification for two new wood-inhabiting species of Botryobasidium (Basidiomycota) from ChinaXin Li0Xin Zhang1Yi-Fei Sun2Zhen-Hao Li3An-Hong Zhu4Ying-Da Wu5Ministry of Emergency Management, China Fire and Rescue InstituteBeijing Forestry UniversityBeijing Forestry UniversityZhejiang Key Laboratory of Biological Breeding and Exploitation of Edible and Medicinal MushroomsCoconut Research Institute, Chinese Academy of Tropical Agricultural SciencesMinistry of Emergency Management, China Fire and Rescue InstituteThe wood-inhabiting fungi refer to large basidiomycetes that grow on various woody materials and are distributed in various forest ecosystems, some of which have important economic value. In the present study, two new resupinate, adnate, wood-inhabiting fungal taxa, Botryobasidium latihyphum and B. zhejiangensis, are introduced based on morphological and molecular characteristics. A molecular phylogenetic study based on sequence data from the internal transcribed spacers (ITS) and the large subunit (nLSU) regions supported the two new species in the genus Botryobasidium. Maximum likelihood (ML), maximum parsimony (MP), and Bayesian inference (BIBI) were employed to perform phylogenetic analyses of these datasets. The new species B. latihyphum is characterized by its cream hymenial surface when fresh, olivaceous buff when dry, a monomitic hyphal system with clamp connections, the presence of clavate to tubular cystidia, basidia with six sterigmata, and broadly oval basidiospores measuring 7.9–10.2 × 3.2–4.3 μm. Botryobasidium zhejiangensis sp. nov. is characterized by its white to buff-yellow hymenial surface when fresh, cream when dry, a monomitic hyphal system with clamp connections, lacking cystidia, basidia with six sterigmata, and broadly navicular basidiospores measuring 7.9–9.2 × 2.6–3.4 μm. The phylogenetic result inferred from ITS + nLSU sequence data revealed that B. latihyphum is closely related to B. vagum, B. laeve, B. subincanum, and B. incanum, while B. zhejiangensis is closely related to B. leptocystidiatum, B. subcoronatum, B. xizangense, and B. intertextum.https://mycokeys.pensoft.net/article/143594/download/pdf/
spellingShingle Xin Li
Xin Zhang
Yi-Fei Sun
Zhen-Hao Li
An-Hong Zhu
Ying-Da Wu
Morphological and molecular identification for two new wood-inhabiting species of Botryobasidium (Basidiomycota) from China
MycoKeys
title Morphological and molecular identification for two new wood-inhabiting species of Botryobasidium (Basidiomycota) from China
title_full Morphological and molecular identification for two new wood-inhabiting species of Botryobasidium (Basidiomycota) from China
title_fullStr Morphological and molecular identification for two new wood-inhabiting species of Botryobasidium (Basidiomycota) from China
title_full_unstemmed Morphological and molecular identification for two new wood-inhabiting species of Botryobasidium (Basidiomycota) from China
title_short Morphological and molecular identification for two new wood-inhabiting species of Botryobasidium (Basidiomycota) from China
title_sort morphological and molecular identification for two new wood inhabiting species of botryobasidium basidiomycota from china
url https://mycokeys.pensoft.net/article/143594/download/pdf/
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