Positive Effects of Lycopene on Growth Performance, Hepatic Antioxidant Capacity, Intestinal Morphology, and Cecal Microflora of Yellow-Feather Broilers

Lycopene (Lyc) possesses strong antioxidant activity through its ability to scavenge singlet oxygen and modulate immune responses in livestock and poultry. As few studies have examined the effects of Lyc on hepatic antioxidant capacity in broilers, we evaluated the effects of dietary Lyc supplementa...

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Main Authors: Guangtian Cao, Xiyue Liu, Huixian Wang, Jia Kang, Feiyang Wang, Molin Li, Wenqi Sun, Xiaosi Lv
Format: Article
Language:English
Published: MDPI AG 2025-07-01
Series:Animals
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Online Access:https://www.mdpi.com/2076-2615/15/14/2108
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author Guangtian Cao
Xiyue Liu
Huixian Wang
Jia Kang
Feiyang Wang
Molin Li
Wenqi Sun
Xiaosi Lv
author_facet Guangtian Cao
Xiyue Liu
Huixian Wang
Jia Kang
Feiyang Wang
Molin Li
Wenqi Sun
Xiaosi Lv
author_sort Guangtian Cao
collection DOAJ
description Lycopene (Lyc) possesses strong antioxidant activity through its ability to scavenge singlet oxygen and modulate immune responses in livestock and poultry. As few studies have examined the effects of Lyc on hepatic antioxidant capacity in broilers, we evaluated the effects of dietary Lyc supplementation on growth performance, antioxidant capacity, intestinal morphology, and microflora composition in yellow-feather broilers. We randomly assigned 480 one-day-old yellow-feather broilers to four dietary treatments: a basal diet with no additive (Con), and a basal diet supplemented with 150 mg/kg (Lyc150), 250 mg/kg (Lyc250), or 500 mg/kg (Lyc500) of lycopene. Compared to Con, Lyc supplementations significantly increased average daily gain by more than 14% from days 29 to 56. Additionally, Lyc250 significantly increased by over 6.4% in average daily feed intake from days 1 to 28, as well as by over 50% in hepatic levels of total antioxidant capacity and 65% in catalase on day 56. Lyc250 significantly increased villus height and decreased the villus-to-crypt ratio. Moreover, both Lyc250 and Lyc500 significantly downregulated hepatic Bax mRNA expression. Further, Lyc150 and Lyc250 significantly increased the relative abundance of <i>Fermentum_g_Lactobacilus</i>, <i>Enterococcus_cecorum_g_Enterococcus</i>, and <i>Ruminococcus sp_g_Ruminococcus</i>, but decreased <i>Acetobacter_lovaniensis_g_Acetobacter</i> and <i>Lactobacillus_amylolyicus_g_Lactobacilus</i>. Overall, our study found that Lyc supplementation significantly improved growth performance, enhanced hepatic antioxidant capacity, and modulated cecal microflora through increasing beneficial species in yellow-feather broilers.
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spelling doaj-art-df0851e8e39e4067b9c01bc198a3c2822025-08-20T03:13:42ZengMDPI AGAnimals2076-26152025-07-011514210810.3390/ani15142108Positive Effects of Lycopene on Growth Performance, Hepatic Antioxidant Capacity, Intestinal Morphology, and Cecal Microflora of Yellow-Feather BroilersGuangtian Cao0Xiyue Liu1Huixian Wang2Jia Kang3Feiyang Wang4Molin Li5Wenqi Sun6Xiaosi Lv7College of Quality and Standardisation, China Jiliang Universtiy, Hangzhou 310058, ChinaDepartment of Life Sciences, Imperial College London, London SW7 2AZ, UKKey Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, Zhejiang Provincial Engineering Laboratory for Animal Health and Internet Technology, College of Animal Science and Technology, Zhejiang A & F University, Hangzhou 311300, ChinaKey Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, Zhejiang Provincial Engineering Laboratory for Animal Health and Internet Technology, College of Animal Science and Technology, Zhejiang A & F University, Hangzhou 311300, ChinaCollege of Quality and Standardisation, China Jiliang Universtiy, Hangzhou 310058, ChinaCollege of Quality and Standardisation, China Jiliang Universtiy, Hangzhou 310058, ChinaCollege of Quality and Standardisation, China Jiliang Universtiy, Hangzhou 310058, ChinaZhejiang Institute of Quality Sciences, Hangzhou 310018, ChinaLycopene (Lyc) possesses strong antioxidant activity through its ability to scavenge singlet oxygen and modulate immune responses in livestock and poultry. As few studies have examined the effects of Lyc on hepatic antioxidant capacity in broilers, we evaluated the effects of dietary Lyc supplementation on growth performance, antioxidant capacity, intestinal morphology, and microflora composition in yellow-feather broilers. We randomly assigned 480 one-day-old yellow-feather broilers to four dietary treatments: a basal diet with no additive (Con), and a basal diet supplemented with 150 mg/kg (Lyc150), 250 mg/kg (Lyc250), or 500 mg/kg (Lyc500) of lycopene. Compared to Con, Lyc supplementations significantly increased average daily gain by more than 14% from days 29 to 56. Additionally, Lyc250 significantly increased by over 6.4% in average daily feed intake from days 1 to 28, as well as by over 50% in hepatic levels of total antioxidant capacity and 65% in catalase on day 56. Lyc250 significantly increased villus height and decreased the villus-to-crypt ratio. Moreover, both Lyc250 and Lyc500 significantly downregulated hepatic Bax mRNA expression. Further, Lyc150 and Lyc250 significantly increased the relative abundance of <i>Fermentum_g_Lactobacilus</i>, <i>Enterococcus_cecorum_g_Enterococcus</i>, and <i>Ruminococcus sp_g_Ruminococcus</i>, but decreased <i>Acetobacter_lovaniensis_g_Acetobacter</i> and <i>Lactobacillus_amylolyicus_g_Lactobacilus</i>. Overall, our study found that Lyc supplementation significantly improved growth performance, enhanced hepatic antioxidant capacity, and modulated cecal microflora through increasing beneficial species in yellow-feather broilers.https://www.mdpi.com/2076-2615/15/14/2108lycopeneyellow-feather broilersantioxidant capacityintestinal morphologycecal microflora
spellingShingle Guangtian Cao
Xiyue Liu
Huixian Wang
Jia Kang
Feiyang Wang
Molin Li
Wenqi Sun
Xiaosi Lv
Positive Effects of Lycopene on Growth Performance, Hepatic Antioxidant Capacity, Intestinal Morphology, and Cecal Microflora of Yellow-Feather Broilers
Animals
lycopene
yellow-feather broilers
antioxidant capacity
intestinal morphology
cecal microflora
title Positive Effects of Lycopene on Growth Performance, Hepatic Antioxidant Capacity, Intestinal Morphology, and Cecal Microflora of Yellow-Feather Broilers
title_full Positive Effects of Lycopene on Growth Performance, Hepatic Antioxidant Capacity, Intestinal Morphology, and Cecal Microflora of Yellow-Feather Broilers
title_fullStr Positive Effects of Lycopene on Growth Performance, Hepatic Antioxidant Capacity, Intestinal Morphology, and Cecal Microflora of Yellow-Feather Broilers
title_full_unstemmed Positive Effects of Lycopene on Growth Performance, Hepatic Antioxidant Capacity, Intestinal Morphology, and Cecal Microflora of Yellow-Feather Broilers
title_short Positive Effects of Lycopene on Growth Performance, Hepatic Antioxidant Capacity, Intestinal Morphology, and Cecal Microflora of Yellow-Feather Broilers
title_sort positive effects of lycopene on growth performance hepatic antioxidant capacity intestinal morphology and cecal microflora of yellow feather broilers
topic lycopene
yellow-feather broilers
antioxidant capacity
intestinal morphology
cecal microflora
url https://www.mdpi.com/2076-2615/15/14/2108
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