Entire chloroplast genome sequence of tea (Camellia sinensis cv. Longjing 43): a molecular phylogenetic analysis
Camellia sinensis cv. Longjing 43 is a domestic variety of tea species and an important economic crop in China. In this study, we developed a rapid method to get the chloroplast (cp) genome and sequenced the entire cp genome sequence of C. sinensis cv. Longjing 43. The C. sinensis cv. Longjing 43 cp...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2014-07-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2014.05.051 |
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| Summary: | Camellia sinensis cv. Longjing 43 is a domestic variety of tea species and an important economic crop in China. In this study, we developed a rapid method to get the chloroplast (cp) genome and sequenced the entire cp genome sequence of C. sinensis cv. Longjing 43. The C. sinensis cv. Longjing 43 cp genome was 157 085 bp in length, which contained a large single-copy (LSC, 86 642 bp) region, a small single-copy (SSC, 18 283 bp) region, and two inverted repeat (IR, each with a size of 26 080 bp) regions. With the cp genome of Korean C. sinensis cultivar as a reference, 134 chloroplast genes were successfully annotated. There were 15 genes with non-synonymous mutations in the coding region and more than 100 polymorphic sites in the non-coding region, which could be the DNA markers for the determination of different C. sinensis varieties. We also investigated the relationship of 12 C. sinensis varieties in China based on several cp genomic regions, which contain many variant sites. The result showed that these varieties were divided into two groups with Lingyunbaimaocha in one group and the other 11 in another group. Among the other 11 varieties, the Longjingchangye, Longjingyuanye, Longjingguazi, and Zhongcha 102 had a closer relationship and were formed into one cluster with 100% support rate, demonstrating the reliability of the method that used the cp genome sequences to investigate the genetic relationships. |
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| ISSN: | 1008-9209 2097-5155 |