Determination of Flavanones in Orange Juices Obtained from Different Sources by HPLC/DAD
Flavanones (hesperidin, naringenin, naringin, and poncirin) in industrial, hand-squeezed orange juices and from fresh-in-squeeze machines orange juices were determined by HPLC/DAD analysis using a previously described liquid-liquid extraction method. Method validation including the accuracy was perf...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2014/296838 |
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| author | Lidércia C. R. Cerqueira e Silva Jorge M. David Rafael dos S. Q. Borges Sérgio L. C. Ferreira Juceni P. David Pedro S. dos Reis Roy E. Bruns |
| author_facet | Lidércia C. R. Cerqueira e Silva Jorge M. David Rafael dos S. Q. Borges Sérgio L. C. Ferreira Juceni P. David Pedro S. dos Reis Roy E. Bruns |
| author_sort | Lidércia C. R. Cerqueira e Silva |
| collection | DOAJ |
| description | Flavanones (hesperidin, naringenin, naringin, and poncirin) in industrial, hand-squeezed orange juices and from fresh-in-squeeze machines orange juices were determined by HPLC/DAD analysis using a previously described liquid-liquid extraction method. Method validation including the accuracy was performed by using recovery tests. Samples (36) collected from different Brazilian locations and brands were analyzed. Concentrations were determined using an external standard curve. The limits of detection (LOD) and the limits of quantification (LOQ) calculated were 0.0037, 1.87, 0.0147, and 0.0066 mg 100 g−1 and 0.0089, 7.84, 0.0302, and 0.0200 mg 100 g−1 for naringin, hesperidin, poncirin, and naringenin, respectively. The results demonstrated that hesperidin was present at the highest concentration levels, especially in the industrial orange juices. Its average content and concentration range were 69.85 and 18.80–139.00 mg 100 g−1. The other flavanones showed the lowest concentration levels. The average contents and concentration ranges found were 0.019, 0.01–0.30, and 0.12 and 0.1–0.17, 0.13, and 0.01–0.36 mg 100 g−1, respectively. The results were also evaluated using the principal component analysis (PCA) multivariate analysis technique which showed that poncirin, naringenin, and naringin were the principal elements that contributed to the variability in the sample concentrations. |
| format | Article |
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| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-def04fd402fe4fc4a293403c2bfa5e0d2025-02-03T01:10:38ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732014-01-01201410.1155/2014/296838296838Determination of Flavanones in Orange Juices Obtained from Different Sources by HPLC/DADLidércia C. R. Cerqueira e Silva0Jorge M. David1Rafael dos S. Q. Borges2Sérgio L. C. Ferreira3Juceni P. David4Pedro S. dos Reis5Roy E. Bruns6Universidade Federal da Bahia, Instituto de Química, 40170-290 Salvador, BA, BrazilUniversidade Federal da Bahia, Instituto de Química, 40170-290 Salvador, BA, BrazilUniversidade Federal da Bahia, Instituto de Química, 40170-290 Salvador, BA, BrazilUniversidade Federal da Bahia, Instituto de Química, 40170-290 Salvador, BA, BrazilUniversidade Federal da Bahia, Faculdade de Farmácia, 40170-290 Salvador, BA, BrazilUniversidade Federal da Bahia, Instituto de Química, 40170-290 Salvador, BA, BrazilUniversidade Estadual de Campinas, Instituto de Química, 13084-971 Campinas, SP, BrazilFlavanones (hesperidin, naringenin, naringin, and poncirin) in industrial, hand-squeezed orange juices and from fresh-in-squeeze machines orange juices were determined by HPLC/DAD analysis using a previously described liquid-liquid extraction method. Method validation including the accuracy was performed by using recovery tests. Samples (36) collected from different Brazilian locations and brands were analyzed. Concentrations were determined using an external standard curve. The limits of detection (LOD) and the limits of quantification (LOQ) calculated were 0.0037, 1.87, 0.0147, and 0.0066 mg 100 g−1 and 0.0089, 7.84, 0.0302, and 0.0200 mg 100 g−1 for naringin, hesperidin, poncirin, and naringenin, respectively. The results demonstrated that hesperidin was present at the highest concentration levels, especially in the industrial orange juices. Its average content and concentration range were 69.85 and 18.80–139.00 mg 100 g−1. The other flavanones showed the lowest concentration levels. The average contents and concentration ranges found were 0.019, 0.01–0.30, and 0.12 and 0.1–0.17, 0.13, and 0.01–0.36 mg 100 g−1, respectively. The results were also evaluated using the principal component analysis (PCA) multivariate analysis technique which showed that poncirin, naringenin, and naringin were the principal elements that contributed to the variability in the sample concentrations.http://dx.doi.org/10.1155/2014/296838 |
| spellingShingle | Lidércia C. R. Cerqueira e Silva Jorge M. David Rafael dos S. Q. Borges Sérgio L. C. Ferreira Juceni P. David Pedro S. dos Reis Roy E. Bruns Determination of Flavanones in Orange Juices Obtained from Different Sources by HPLC/DAD Journal of Analytical Methods in Chemistry |
| title | Determination of Flavanones in Orange Juices Obtained from Different Sources by HPLC/DAD |
| title_full | Determination of Flavanones in Orange Juices Obtained from Different Sources by HPLC/DAD |
| title_fullStr | Determination of Flavanones in Orange Juices Obtained from Different Sources by HPLC/DAD |
| title_full_unstemmed | Determination of Flavanones in Orange Juices Obtained from Different Sources by HPLC/DAD |
| title_short | Determination of Flavanones in Orange Juices Obtained from Different Sources by HPLC/DAD |
| title_sort | determination of flavanones in orange juices obtained from different sources by hplc dad |
| url | http://dx.doi.org/10.1155/2014/296838 |
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