A comprehensive analysis of the prognostic value, expression characteristics and immune correlation of MKI67 in cancers

Backgroundnuclear-associated antigen Ki67 (Ki67) emerges as a clinically practical biomarker for proliferation assessment among many cancer types. However, the definite prognostic value of Ki67 against a specific cancer type has remained vague. This study aims to perform a comprehensive pan-cancer a...

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Main Authors: Xiaolan Pan, Caibiao Wei, Jingyu Su, Min Fang, Qiumei Lin, Yuling Qin, Jie Gao, Jie Zhao, Huiliu Zhao, Fengfei Liu
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-02-01
Series:Frontiers in Immunology
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Online Access:https://www.frontiersin.org/articles/10.3389/fimmu.2025.1531708/full
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author Xiaolan Pan
Caibiao Wei
Jingyu Su
Min Fang
Qiumei Lin
Yuling Qin
Jie Gao
Jie Zhao
Huiliu Zhao
Fengfei Liu
author_facet Xiaolan Pan
Caibiao Wei
Jingyu Su
Min Fang
Qiumei Lin
Yuling Qin
Jie Gao
Jie Zhao
Huiliu Zhao
Fengfei Liu
author_sort Xiaolan Pan
collection DOAJ
description Backgroundnuclear-associated antigen Ki67 (Ki67) emerges as a clinically practical biomarker for proliferation assessment among many cancer types. However, the definite prognostic value of Ki67 against a specific cancer type has remained vague. This study aims to perform a comprehensive pan-cancer analysis of the prognosis value of Ki67 across various cancer types.MethodsThis study explored the expression, prognostic value, and tumor-infiltrating immune of MKI67 in the TCGA database by pan-cancer, and then performed immunohistochemical, correlation analysis and prognostic analysis using 10028 patients of the top 10 cancer patients in China we collected. The correlation between MKI67 expression and survival outcome, clinical features, MSI, TMB, and tumor-infiltrating immune cells by TCGA database, xCell, and TIMER algorithms.ResultsMKI67 expression was significantly upregulated across varied cancer types verified by datasets. We found MKI67 expression was significantly associated with poor prognosis in LUADLUSC, LIHC, and BRCA patients, but good prognosis in COADREAD and READ patients via Kaplan-Meier survival analysis using 10028 patients collected. These results of our validation were generally consistent with TCGA database except BRCA, COADREAD and READ. Meanwhile, upregulation of MKI67 elevates the degree of immune infiltration of several immune cell subtypes, such as functional T cells, CD4+ T cells, and CD8+ T cells, as well as, MKI67 was related to Cell cycle, Oocyte meiosis, p53 and other pathways.ConclusionOur comprehensive analysis may supply useful guidance on MKI67 applicability across various cancer types. These observed results contribute to the promise of MKI67 in a realistic clinical setting and improve the outcomes of cancer patients.
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spelling doaj-art-dee268ead52b4b19b7d8a88dca844b422025-08-20T02:44:50ZengFrontiers Media S.A.Frontiers in Immunology1664-32242025-02-011610.3389/fimmu.2025.15317081531708A comprehensive analysis of the prognostic value, expression characteristics and immune correlation of MKI67 in cancersXiaolan Pan0Caibiao Wei1Jingyu Su2Min Fang3Qiumei Lin4Yuling Qin5Jie Gao6Jie Zhao7Huiliu Zhao8Fengfei Liu9Department of Clinical Laboratory, Guangxi Medical University Cancer Hospital, Nanning, ChinaDepartment of Clinical Laboratory, Guangxi Medical University Cancer Hospital, Nanning, ChinaGenetic Metabolism Center laboratory, Guangxi Zhuang Autonomous Region Maternal and Child Health Care Hospital, Nanning, ChinaDepartment of Clinical Laboratory, Guangxi Medical University Cancer Hospital, Nanning, ChinaDepartment of Clinical Laboratory, Guangxi Medical University Cancer Hospital, Nanning, ChinaDepartment of Clinical Laboratory, Guangxi Medical University Cancer Hospital, Nanning, ChinaDepartment of Clinical Laboratory, Guangxi Medical University Cancer Hospital, Nanning, ChinaDepartment of Medical Records, Guangxi Medical University Cancer Hospital, Nanning, ChinaDepartment of Clinical Laboratory, Guangxi Medical University Cancer Hospital, Nanning, ChinaDepartment of Clinical Laboratory, Guangxi Medical University Cancer Hospital, Nanning, ChinaBackgroundnuclear-associated antigen Ki67 (Ki67) emerges as a clinically practical biomarker for proliferation assessment among many cancer types. However, the definite prognostic value of Ki67 against a specific cancer type has remained vague. This study aims to perform a comprehensive pan-cancer analysis of the prognosis value of Ki67 across various cancer types.MethodsThis study explored the expression, prognostic value, and tumor-infiltrating immune of MKI67 in the TCGA database by pan-cancer, and then performed immunohistochemical, correlation analysis and prognostic analysis using 10028 patients of the top 10 cancer patients in China we collected. The correlation between MKI67 expression and survival outcome, clinical features, MSI, TMB, and tumor-infiltrating immune cells by TCGA database, xCell, and TIMER algorithms.ResultsMKI67 expression was significantly upregulated across varied cancer types verified by datasets. We found MKI67 expression was significantly associated with poor prognosis in LUADLUSC, LIHC, and BRCA patients, but good prognosis in COADREAD and READ patients via Kaplan-Meier survival analysis using 10028 patients collected. These results of our validation were generally consistent with TCGA database except BRCA, COADREAD and READ. Meanwhile, upregulation of MKI67 elevates the degree of immune infiltration of several immune cell subtypes, such as functional T cells, CD4+ T cells, and CD8+ T cells, as well as, MKI67 was related to Cell cycle, Oocyte meiosis, p53 and other pathways.ConclusionOur comprehensive analysis may supply useful guidance on MKI67 applicability across various cancer types. These observed results contribute to the promise of MKI67 in a realistic clinical setting and improve the outcomes of cancer patients.https://www.frontiersin.org/articles/10.3389/fimmu.2025.1531708/fullMKI67prognosiscancer immunitypathwaymulti-omics bioinformatics
spellingShingle Xiaolan Pan
Caibiao Wei
Jingyu Su
Min Fang
Qiumei Lin
Yuling Qin
Jie Gao
Jie Zhao
Huiliu Zhao
Fengfei Liu
A comprehensive analysis of the prognostic value, expression characteristics and immune correlation of MKI67 in cancers
Frontiers in Immunology
MKI67
prognosis
cancer immunity
pathway
multi-omics bioinformatics
title A comprehensive analysis of the prognostic value, expression characteristics and immune correlation of MKI67 in cancers
title_full A comprehensive analysis of the prognostic value, expression characteristics and immune correlation of MKI67 in cancers
title_fullStr A comprehensive analysis of the prognostic value, expression characteristics and immune correlation of MKI67 in cancers
title_full_unstemmed A comprehensive analysis of the prognostic value, expression characteristics and immune correlation of MKI67 in cancers
title_short A comprehensive analysis of the prognostic value, expression characteristics and immune correlation of MKI67 in cancers
title_sort comprehensive analysis of the prognostic value expression characteristics and immune correlation of mki67 in cancers
topic MKI67
prognosis
cancer immunity
pathway
multi-omics bioinformatics
url https://www.frontiersin.org/articles/10.3389/fimmu.2025.1531708/full
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