Molecular characterization of the evolution of phagosomes
Abstract Amoeba use phagocytosis to internalize bacteria as a source of nutrients, whereas multicellular organisms utilize this process as a defense mechanism to kill microbes and, in vertebrates, initiate a sustained immune response. By using a large‐scale approach to identify and compare the prote...
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| Format: | Article |
| Language: | English |
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Springer Nature
2010-10-01
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| Series: | Molecular Systems Biology |
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| Online Access: | https://doi.org/10.1038/msb.2010.80 |
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| _version_ | 1849225802742235136 |
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| author | Jonathan Boulais Matthias Trost Christian R Landry Régis Dieckmann Emmanuel D Levy Thierry Soldati Stephen W Michnick Pierre Thibault Michel Desjardins |
| author_facet | Jonathan Boulais Matthias Trost Christian R Landry Régis Dieckmann Emmanuel D Levy Thierry Soldati Stephen W Michnick Pierre Thibault Michel Desjardins |
| author_sort | Jonathan Boulais |
| collection | DOAJ |
| description | Abstract Amoeba use phagocytosis to internalize bacteria as a source of nutrients, whereas multicellular organisms utilize this process as a defense mechanism to kill microbes and, in vertebrates, initiate a sustained immune response. By using a large‐scale approach to identify and compare the proteome and phosphoproteome of phagosomes isolated from distant organisms, and by comparative analysis over 39 taxa, we identified an ‘ancient’ core of phagosomal proteins around which the immune functions of this organelle have likely organized. Our data indicate that a larger proportion of the phagosome proteome, compared with the whole cell proteome, has been acquired through gene duplication at a period coinciding with the emergence of innate and adaptive immunity. Our study also characterizes in detail the acquisition of novel proteins and the significant remodeling of the phagosome phosphoproteome that contributed to modify the core constituents of this organelle in evolution. Our work thus provides the first thorough analysis of the changes that enabled the transformation of the phagosome from a phagotrophic compartment into an organelle fully competent for antigen presentation. |
| format | Article |
| id | doaj-art-dedc093817f64871a4e156e6ef64cae3 |
| institution | Kabale University |
| issn | 1744-4292 |
| language | English |
| publishDate | 2010-10-01 |
| publisher | Springer Nature |
| record_format | Article |
| series | Molecular Systems Biology |
| spelling | doaj-art-dedc093817f64871a4e156e6ef64cae32025-08-24T11:59:59ZengSpringer NatureMolecular Systems Biology1744-42922010-10-016111410.1038/msb.2010.80Molecular characterization of the evolution of phagosomesJonathan Boulais0Matthias Trost1Christian R Landry2Régis Dieckmann3Emmanuel D Levy4Thierry Soldati5Stephen W Michnick6Pierre Thibault7Michel Desjardins8Département de Pathologie et Biologie Cellulaire, Université de Montréal, MontréalMRC Protein Phosphorylation Unit, College of Life Sciences, University of DundeeDépartement de Biologie, PROTEO and Institut de Biologie Intégrative et des Systèmes (IBIS), Université Laval, QuébecDepartment of Biochemistry, University of GenevaDépartement de Biochimie, Université de Montréal, MontréalDepartment of Biochemistry, University of GenevaDépartement de Biochimie, Université de Montréal, MontréalUnité protéomique et spectrométrie de masse bioanalytique, Institut de Recherche en Immunologie et Cancérologie (IRIC), Université de Montréal, MontréalDépartement de Pathologie et Biologie Cellulaire, Université de Montréal, MontréalAbstract Amoeba use phagocytosis to internalize bacteria as a source of nutrients, whereas multicellular organisms utilize this process as a defense mechanism to kill microbes and, in vertebrates, initiate a sustained immune response. By using a large‐scale approach to identify and compare the proteome and phosphoproteome of phagosomes isolated from distant organisms, and by comparative analysis over 39 taxa, we identified an ‘ancient’ core of phagosomal proteins around which the immune functions of this organelle have likely organized. Our data indicate that a larger proportion of the phagosome proteome, compared with the whole cell proteome, has been acquired through gene duplication at a period coinciding with the emergence of innate and adaptive immunity. Our study also characterizes in detail the acquisition of novel proteins and the significant remodeling of the phagosome phosphoproteome that contributed to modify the core constituents of this organelle in evolution. Our work thus provides the first thorough analysis of the changes that enabled the transformation of the phagosome from a phagotrophic compartment into an organelle fully competent for antigen presentation.https://doi.org/10.1038/msb.2010.80evolutionimmunityphosphoproteomicsphylogenyproteomics |
| spellingShingle | Jonathan Boulais Matthias Trost Christian R Landry Régis Dieckmann Emmanuel D Levy Thierry Soldati Stephen W Michnick Pierre Thibault Michel Desjardins Molecular characterization of the evolution of phagosomes Molecular Systems Biology evolution immunity phosphoproteomics phylogeny proteomics |
| title | Molecular characterization of the evolution of phagosomes |
| title_full | Molecular characterization of the evolution of phagosomes |
| title_fullStr | Molecular characterization of the evolution of phagosomes |
| title_full_unstemmed | Molecular characterization of the evolution of phagosomes |
| title_short | Molecular characterization of the evolution of phagosomes |
| title_sort | molecular characterization of the evolution of phagosomes |
| topic | evolution immunity phosphoproteomics phylogeny proteomics |
| url | https://doi.org/10.1038/msb.2010.80 |
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