Phytochemical Analysis, Antioxidant Activity, Antimicrobial Activity, and Cytotoxicity of Chaptalia nutans Leaves
Context. Chaptalia nutans (L.) Pol. (family: Asteraceae) is widely used in traditional medicine as laxative and anticough medications and especially in the traumatisms, wounds, and hemorrhages in topical preparations. Objective. This work was to evaluate the chemical constitution of the hydromethano...
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Wiley
2020-01-01
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| Series: | Advances in Pharmacological and Pharmaceutical Sciences |
| Online Access: | http://dx.doi.org/10.1155/2020/3260745 |
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| author | Letiele Bruck de Souza Amanda Leitão Gindri Thainara de Andrade Fortes Thais Felli Kubiça Jefferson Enderle Rafael Roehrs Sidnei Moura e Silva Vanusa Manfredini Elton Luís Gasparotto Denardin |
| author_facet | Letiele Bruck de Souza Amanda Leitão Gindri Thainara de Andrade Fortes Thais Felli Kubiça Jefferson Enderle Rafael Roehrs Sidnei Moura e Silva Vanusa Manfredini Elton Luís Gasparotto Denardin |
| author_sort | Letiele Bruck de Souza |
| collection | DOAJ |
| description | Context. Chaptalia nutans (L.) Pol. (family: Asteraceae) is widely used in traditional medicine as laxative and anticough medications and especially in the traumatisms, wounds, and hemorrhages in topical preparations. Objective. This work was to evaluate the chemical constitution of the hydromethanolic (30/70 methanol-water) macerating extract obtained from the leaves of C. nutans, as well as to study the antioxidant, antimicrobial, cytotoxic, and genotoxic activity of the species. Materials and methods. Phytochemical screening, antioxidant activity (total phenolic, total flavonoid, condensed tannins content, DPPH radical, and FRAP), antibacterial activity (P. aeruginosa, B. cereus, E. epidermidis, E. coli, S. aureus, E. faecalis, P. mirabilis, Candida glabrata (clinical isolate), Candida tropicalis (clinical isolate), C. krusei (clinical isolate), and C. albicans (clinical isolate)), and oxidative stress parameters (TBARS, carbonyl protein, and DCFH) were analyzed according to the literature. Toxicity of C. nutans was evaluated using an alternative method, D. melanogaster, as well as a locomotor assay. Results. The phytochemical screening test of methanolic leaves extract revealed the presence of alkaloids, coumarins, quaternary bases, phenolics, flavonoids, tannins, and free steroids. A quantitative phytochemical study indicated the total phenol (30.17 ± 1.44 mg/g), flavonoid (21.64 ± 0.66 mg/g), and condensed tannins (9.58 ± 0.99 mg/g). DPPH (345.41 ± 5.35 μg/mL) and FRAP (379.98 ± 39.25 μM FeSO4/mg sample) show to extract of C. nutans leaves an intermediate value, indicating moderate antioxidant activity of the extract. Antibacterial results revealed only a positive result (antimicrobial activity) for the hexane fraction which significantly inhibited the microorganisms E. epidermidis, C. tropicalis, C. glabrata, and C. krusei at a concentration of 1000 μg/mL. TBARS, carbonyl protein, and DCFH demonstrate that the extract has the ability to protect the cell from protein and lipid damage, as well as the inhibition of oxygen-derived radicals at the three concentrations tested: 0.1, 1, and 10 mg/mL. Regarding the toxicity of C. nutans extract against D. melanogaster, it was found that until the concentration of 15 mg/mL, the extract showed no toxicity and that the LC50 obtained was 24 mg/mL. Results show that the C. nutans extract leaves used to prevent PQ damage were effective in reducing flies’ mortality and improving locomotor capacity. Conclusion. Our studies demonstrated for the first time that C. nutans crude leaf extract has high antioxidant capacity both in vitro and in vivo through different analysis techniques. These results make it possible to infer future applications in the pharmacological area, evidenced by the low toxicity observed in D. melanogatser, as well as the ability to neutralize different sources of RONS. |
| format | Article |
| id | doaj-art-ded7884cb3a9405b8ffb53c9613bb184 |
| institution | Kabale University |
| issn | 2633-4690 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advances in Pharmacological and Pharmaceutical Sciences |
| spelling | doaj-art-ded7884cb3a9405b8ffb53c9613bb1842025-02-03T01:05:09ZengWileyAdvances in Pharmacological and Pharmaceutical Sciences2633-46902020-01-01202010.1155/2020/32607453260745Phytochemical Analysis, Antioxidant Activity, Antimicrobial Activity, and Cytotoxicity of Chaptalia nutans LeavesLetiele Bruck de Souza0Amanda Leitão Gindri1Thainara de Andrade Fortes2Thais Felli Kubiça3Jefferson Enderle4Rafael Roehrs5Sidnei Moura e Silva6Vanusa Manfredini7Elton Luís Gasparotto Denardin8Laboratório Estudos Físico–Químico e Produtos Naturais (LEFQPN), Federal University of Pampa (UNIPAMPA), P. Box 118, Uruguaiana, RS 97501-970, BrazilLaboratório de Plantas Medicinais, Universidade Regional Integrada do Alto Uruguai e Das Missões, Santiago, RS, BrazilLaboratório de Plantas Medicinais, Universidade Regional Integrada do Alto Uruguai e Das Missões, Santiago, RS, BrazilLaboratório de Plantas Medicinais, Universidade Regional Integrada do Alto Uruguai e Das Missões, Santiago, RS, BrazilLaboratório Estudos Físico–Químico e Produtos Naturais (LEFQPN), Federal University of Pampa (UNIPAMPA), P. Box 118, Uruguaiana, RS 97501-970, BrazilLaboratório Estudos Físico–Químico e Produtos Naturais (LEFQPN), Federal University of Pampa (UNIPAMPA), P. Box 118, Uruguaiana, RS 97501-970, BrazilUniversidade de Caxias do Sul (UCS), Caxias do Sul, RS, BrazilGrupo de Estudos em Estresse Oxidativo (GESTOX), Federal University of Pampa (UNIPAMPA), Uruguaiana, RS, BrazilLaboratório Estudos Físico–Químico e Produtos Naturais (LEFQPN), Federal University of Pampa (UNIPAMPA), P. Box 118, Uruguaiana, RS 97501-970, BrazilContext. Chaptalia nutans (L.) Pol. (family: Asteraceae) is widely used in traditional medicine as laxative and anticough medications and especially in the traumatisms, wounds, and hemorrhages in topical preparations. Objective. This work was to evaluate the chemical constitution of the hydromethanolic (30/70 methanol-water) macerating extract obtained from the leaves of C. nutans, as well as to study the antioxidant, antimicrobial, cytotoxic, and genotoxic activity of the species. Materials and methods. Phytochemical screening, antioxidant activity (total phenolic, total flavonoid, condensed tannins content, DPPH radical, and FRAP), antibacterial activity (P. aeruginosa, B. cereus, E. epidermidis, E. coli, S. aureus, E. faecalis, P. mirabilis, Candida glabrata (clinical isolate), Candida tropicalis (clinical isolate), C. krusei (clinical isolate), and C. albicans (clinical isolate)), and oxidative stress parameters (TBARS, carbonyl protein, and DCFH) were analyzed according to the literature. Toxicity of C. nutans was evaluated using an alternative method, D. melanogaster, as well as a locomotor assay. Results. The phytochemical screening test of methanolic leaves extract revealed the presence of alkaloids, coumarins, quaternary bases, phenolics, flavonoids, tannins, and free steroids. A quantitative phytochemical study indicated the total phenol (30.17 ± 1.44 mg/g), flavonoid (21.64 ± 0.66 mg/g), and condensed tannins (9.58 ± 0.99 mg/g). DPPH (345.41 ± 5.35 μg/mL) and FRAP (379.98 ± 39.25 μM FeSO4/mg sample) show to extract of C. nutans leaves an intermediate value, indicating moderate antioxidant activity of the extract. Antibacterial results revealed only a positive result (antimicrobial activity) for the hexane fraction which significantly inhibited the microorganisms E. epidermidis, C. tropicalis, C. glabrata, and C. krusei at a concentration of 1000 μg/mL. TBARS, carbonyl protein, and DCFH demonstrate that the extract has the ability to protect the cell from protein and lipid damage, as well as the inhibition of oxygen-derived radicals at the three concentrations tested: 0.1, 1, and 10 mg/mL. Regarding the toxicity of C. nutans extract against D. melanogaster, it was found that until the concentration of 15 mg/mL, the extract showed no toxicity and that the LC50 obtained was 24 mg/mL. Results show that the C. nutans extract leaves used to prevent PQ damage were effective in reducing flies’ mortality and improving locomotor capacity. Conclusion. Our studies demonstrated for the first time that C. nutans crude leaf extract has high antioxidant capacity both in vitro and in vivo through different analysis techniques. These results make it possible to infer future applications in the pharmacological area, evidenced by the low toxicity observed in D. melanogatser, as well as the ability to neutralize different sources of RONS.http://dx.doi.org/10.1155/2020/3260745 |
| spellingShingle | Letiele Bruck de Souza Amanda Leitão Gindri Thainara de Andrade Fortes Thais Felli Kubiça Jefferson Enderle Rafael Roehrs Sidnei Moura e Silva Vanusa Manfredini Elton Luís Gasparotto Denardin Phytochemical Analysis, Antioxidant Activity, Antimicrobial Activity, and Cytotoxicity of Chaptalia nutans Leaves Advances in Pharmacological and Pharmaceutical Sciences |
| title | Phytochemical Analysis, Antioxidant Activity, Antimicrobial Activity, and Cytotoxicity of Chaptalia nutans Leaves |
| title_full | Phytochemical Analysis, Antioxidant Activity, Antimicrobial Activity, and Cytotoxicity of Chaptalia nutans Leaves |
| title_fullStr | Phytochemical Analysis, Antioxidant Activity, Antimicrobial Activity, and Cytotoxicity of Chaptalia nutans Leaves |
| title_full_unstemmed | Phytochemical Analysis, Antioxidant Activity, Antimicrobial Activity, and Cytotoxicity of Chaptalia nutans Leaves |
| title_short | Phytochemical Analysis, Antioxidant Activity, Antimicrobial Activity, and Cytotoxicity of Chaptalia nutans Leaves |
| title_sort | phytochemical analysis antioxidant activity antimicrobial activity and cytotoxicity of chaptalia nutans leaves |
| url | http://dx.doi.org/10.1155/2020/3260745 |
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