Clonal relatedness and resistance patterns of Salmonella Corvallis from poultry carcasses in a Brazilian slaughterhouse
Introduction: Salmonella is a major cause of foodborne disease, and poultry products are important contributors to the transmission of this zoonotic pathogen. Although considered to be rare in most countries, Salmonella Corvallis has been reported in specific geographic areas isolated from both huma...
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The Journal of Infection in Developing Countries
2015-10-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/5634 |
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| author | Ricardo S. Yamatogi Heverton C. Oliveira Carlos H. Camargo Sueli A. Fernandes Rodrigo T. Hernandes José PAN Pinto Vera LM Rall João P. Araújo |
| author_facet | Ricardo S. Yamatogi Heverton C. Oliveira Carlos H. Camargo Sueli A. Fernandes Rodrigo T. Hernandes José PAN Pinto Vera LM Rall João P. Araújo |
| author_sort | Ricardo S. Yamatogi |
| collection | DOAJ |
| description | Introduction: Salmonella is a major cause of foodborne disease, and poultry products are important contributors to the transmission of this zoonotic pathogen. Although considered to be rare in most countries, Salmonella Corvallis has been reported in specific geographic areas isolated from both human and non-human sources. The aim of this study was to report the occurrence, the antimicrobial resistance profiles including the extended-spectrum beta-lactamase (ESBL) production, and the clonal relatedness of S. Corvallis strains.
Methodology: A total of 132 fragments of poultry carcasses from a slaughterhouse in São Paulo State, Brazil, were collected at different stages of the manufacturing process (post-bleeding, post-plucking, and post-chilling) and analyzed for the presence of Salmonella. Antimicrobial resistance was determined by disc diffusion method and Etest. Clonal relatedness was determined by pulsed-field gel electrophoresis (PFGE).
Results: Among the 272 Salmonella strains recovered, fourteen were S. Corvallis. Ten (71.4%) showed ESBL production and resistance to at least three antimicrobial agents. Nalidixic acid resistance and reduced ciprofloxacin susceptibility was verified in four (28.6%) strains. PFGE analyses showed that all the S. Corvallis strains belonged to the same pulsotype.
Conclusion: This study identified genetically related S. Corvallis strains exhibiting ESBL production and reduced susceptibility to quinolone. The results suggest the need to improve the sanitary conditions in the slaughterhouse. Moreover, from a public health perspective, continuous surveillance on Salmonella is needed to control the dissemination of this important zoonotic pathogen and its resistance determinants.
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| format | Article |
| id | doaj-art-decb3f51e2124bb7b557be19f9fb196c |
| institution | OA Journals |
| issn | 1972-2680 |
| language | English |
| publishDate | 2015-10-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-decb3f51e2124bb7b557be19f9fb196c2025-08-20T02:14:18ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802015-10-0191010.3855/jidc.5634Clonal relatedness and resistance patterns of Salmonella Corvallis from poultry carcasses in a Brazilian slaughterhouseRicardo S. Yamatogi0Heverton C. Oliveira1Carlos H. Camargo2Sueli A. Fernandes3Rodrigo T. Hernandes4José PAN Pinto5Vera LM Rall6João P. Araújo7School of Veterinary Medicine and Animal Science, Univ Estadual Paulista – UNESP. Distrito de Rubião Júnior, s/n, Botucatu, SP, BrazilSchool of Veterinary Medicine and Animal Science, Univ Estadual Paulista – UNESP. Distrito de Rubião Júnior, s/n, Botucatu, SP, BrazilAdolfo Lutz Institute. São Paulo, SP, BrazilAdolfo Lutz Institute. São Paulo, SP, BrazilSchool of Veterinary Medicine and Animal Science, Univ Estadual Paulista – UNESP. Distrito de Rubião Júnior, s/n, Botucatu, SP, BrazilSchool of Veterinary Medicine and Animal Science, Univ Estadual Paulista – UNESP. Distrito de Rubião Júnior, s/n, Botucatu, SP, BrazilUniv Estadual Paulista – UNESP. Distrito de Rubião Júnior, s/n, Botucatu, SP, BrazilUniv Estadual Paulista – UNESP. Distrito de Rubião Júnior, s/n, Botucatu, SP, BrazilIntroduction: Salmonella is a major cause of foodborne disease, and poultry products are important contributors to the transmission of this zoonotic pathogen. Although considered to be rare in most countries, Salmonella Corvallis has been reported in specific geographic areas isolated from both human and non-human sources. The aim of this study was to report the occurrence, the antimicrobial resistance profiles including the extended-spectrum beta-lactamase (ESBL) production, and the clonal relatedness of S. Corvallis strains. Methodology: A total of 132 fragments of poultry carcasses from a slaughterhouse in São Paulo State, Brazil, were collected at different stages of the manufacturing process (post-bleeding, post-plucking, and post-chilling) and analyzed for the presence of Salmonella. Antimicrobial resistance was determined by disc diffusion method and Etest. Clonal relatedness was determined by pulsed-field gel electrophoresis (PFGE). Results: Among the 272 Salmonella strains recovered, fourteen were S. Corvallis. Ten (71.4%) showed ESBL production and resistance to at least three antimicrobial agents. Nalidixic acid resistance and reduced ciprofloxacin susceptibility was verified in four (28.6%) strains. PFGE analyses showed that all the S. Corvallis strains belonged to the same pulsotype. Conclusion: This study identified genetically related S. Corvallis strains exhibiting ESBL production and reduced susceptibility to quinolone. The results suggest the need to improve the sanitary conditions in the slaughterhouse. Moreover, from a public health perspective, continuous surveillance on Salmonella is needed to control the dissemination of this important zoonotic pathogen and its resistance determinants. https://jidc.org/index.php/journal/article/view/5634PoultryCorvallisSalmonellaESBLPFGEfluoroquinolones |
| spellingShingle | Ricardo S. Yamatogi Heverton C. Oliveira Carlos H. Camargo Sueli A. Fernandes Rodrigo T. Hernandes José PAN Pinto Vera LM Rall João P. Araújo Clonal relatedness and resistance patterns of Salmonella Corvallis from poultry carcasses in a Brazilian slaughterhouse Journal of Infection in Developing Countries Poultry Corvallis Salmonella ESBL PFGE fluoroquinolones |
| title | Clonal relatedness and resistance patterns of Salmonella Corvallis from poultry carcasses in a Brazilian slaughterhouse |
| title_full | Clonal relatedness and resistance patterns of Salmonella Corvallis from poultry carcasses in a Brazilian slaughterhouse |
| title_fullStr | Clonal relatedness and resistance patterns of Salmonella Corvallis from poultry carcasses in a Brazilian slaughterhouse |
| title_full_unstemmed | Clonal relatedness and resistance patterns of Salmonella Corvallis from poultry carcasses in a Brazilian slaughterhouse |
| title_short | Clonal relatedness and resistance patterns of Salmonella Corvallis from poultry carcasses in a Brazilian slaughterhouse |
| title_sort | clonal relatedness and resistance patterns of salmonella corvallis from poultry carcasses in a brazilian slaughterhouse |
| topic | Poultry Corvallis Salmonella ESBL PFGE fluoroquinolones |
| url | https://jidc.org/index.php/journal/article/view/5634 |
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