A Novel Immunochromatographic Test Strip Using Lanthanide-Labeled Fluorescent Nanoparticles for the Serological Detection of <i>Toxoplasma gondii</i> in Dogs and Cats
<i>Toxoplasma gondii</i> (<i>T. gondii</i>) is an important zoonotic pathogen which induces both acute and chronic toxoplasmosis. Timely diagnosis of <i>T. gondii</i> is crucial for effective disease management. Here, we present a pioneering approach using europiu...
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2024-10-01
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| author | Manyu Zhang Qi Liu Ruifang Li Wei Jiang Hongjin Zhao Wenwei Sheng Luming Xia Zengqiang Li Qing Sun Jingying Du Lei Lei Quan Wang |
| author_facet | Manyu Zhang Qi Liu Ruifang Li Wei Jiang Hongjin Zhao Wenwei Sheng Luming Xia Zengqiang Li Qing Sun Jingying Du Lei Lei Quan Wang |
| author_sort | Manyu Zhang |
| collection | DOAJ |
| description | <i>Toxoplasma gondii</i> (<i>T. gondii</i>) is an important zoonotic pathogen which induces both acute and chronic toxoplasmosis. Timely diagnosis of <i>T. gondii</i> is crucial for effective disease management. Here, we present a pioneering approach using europium (III)-chelated nanoparticles (EuNPs) in a rapid lateral flow immunochromatographic test strip (ICTS) for detecting <i>T. gondii</i> antibodies in serum samples. By conjugating EuNPs with Staphylococcus aureus protein A, we efficiently captured <i>T. gondii</i>-specific antibodies, which bound to <i>T. gondii</i> antigens on the test line (T-line), generating a distinct fluorescent signal. Employing this novel method, we conducted an extensive epidemiological investigation of <i>T. gondii</i> infections among dogs and cats in Shanghai, China. This innovative ICTS allows for rapid results within 25 min, which include a qualitative result through naked-eye observation under an ultraviolet lamp and a quantitative one derived using a strip reader. With a detection limit of 1:6400 for dog positive serum and no cross-reactivity with other canine and feline pathogens, the EuNPs-ICTS demonstrated excellent consistency with standard enzyme-linked immunosorbent assay results for dogs (κ = 0.91) and cats (κ = 0.92). In addition, 20.38% of 996 dog serum samples and 14.18% of 416 cat serum samples revealed <i>T. gondii</i> antibodies, highlighting the efficacy of this approach. Our study presents a rapid, sensitive, specific, and reproducible EuNPs-ICTS, serving as a promising tool for on-the-spot diagnosis of <i>T. gondii</i> infections in dogs and cats. |
| format | Article |
| id | doaj-art-debcc8f0431e41ccabe6c3fd7262dc95 |
| institution | OA Journals |
| issn | 2076-0817 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj-art-debcc8f0431e41ccabe6c3fd7262dc952025-08-20T02:04:59ZengMDPI AGPathogens2076-08172024-10-01131193110.3390/pathogens13110931A Novel Immunochromatographic Test Strip Using Lanthanide-Labeled Fluorescent Nanoparticles for the Serological Detection of <i>Toxoplasma gondii</i> in Dogs and CatsManyu Zhang0Qi Liu1Ruifang Li2Wei Jiang3Hongjin Zhao4Wenwei Sheng5Luming Xia6Zengqiang Li7Qing Sun8Jingying Du9Lei Lei10Quan Wang11Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Science, Shanghai 200241, ChinaInternational Institutes of Medicine, The Fourth Affiliated Hospital, Zhejiang University School of Medicine, Yiwu 322023, ChinaShanghai Veterinary Research Institute, Chinese Academy of Agricultural Science, Shanghai 200241, ChinaShanghai Veterinary Research Institute, Chinese Academy of Agricultural Science, Shanghai 200241, ChinaShanghai Animal Disease Prevention and Control Center, Shanghai 200050, ChinaShanghai Animal Disease Prevention and Control Center, Shanghai 200050, ChinaShanghai Animal Disease Prevention and Control Center, Shanghai 200050, ChinaShanghai Animal Disease Prevention and Control Center, Shanghai 200050, ChinaShanghai Veterinary Research Institute, Chinese Academy of Agricultural Science, Shanghai 200241, ChinaShanghai Veterinary Research Institute, Chinese Academy of Agricultural Science, Shanghai 200241, ChinaShanghai Veterinary Research Institute, Chinese Academy of Agricultural Science, Shanghai 200241, ChinaShanghai Veterinary Research Institute, Chinese Academy of Agricultural Science, Shanghai 200241, China<i>Toxoplasma gondii</i> (<i>T. gondii</i>) is an important zoonotic pathogen which induces both acute and chronic toxoplasmosis. Timely diagnosis of <i>T. gondii</i> is crucial for effective disease management. Here, we present a pioneering approach using europium (III)-chelated nanoparticles (EuNPs) in a rapid lateral flow immunochromatographic test strip (ICTS) for detecting <i>T. gondii</i> antibodies in serum samples. By conjugating EuNPs with Staphylococcus aureus protein A, we efficiently captured <i>T. gondii</i>-specific antibodies, which bound to <i>T. gondii</i> antigens on the test line (T-line), generating a distinct fluorescent signal. Employing this novel method, we conducted an extensive epidemiological investigation of <i>T. gondii</i> infections among dogs and cats in Shanghai, China. This innovative ICTS allows for rapid results within 25 min, which include a qualitative result through naked-eye observation under an ultraviolet lamp and a quantitative one derived using a strip reader. With a detection limit of 1:6400 for dog positive serum and no cross-reactivity with other canine and feline pathogens, the EuNPs-ICTS demonstrated excellent consistency with standard enzyme-linked immunosorbent assay results for dogs (κ = 0.91) and cats (κ = 0.92). In addition, 20.38% of 996 dog serum samples and 14.18% of 416 cat serum samples revealed <i>T. gondii</i> antibodies, highlighting the efficacy of this approach. Our study presents a rapid, sensitive, specific, and reproducible EuNPs-ICTS, serving as a promising tool for on-the-spot diagnosis of <i>T. gondii</i> infections in dogs and cats.https://www.mdpi.com/2076-0817/13/11/931lanthanide-labeled fluorescent nanoparticlesfluorescence immunochromatographic stripsseroprevalence<i>Toxoplasma gondii</i>Shanghai |
| spellingShingle | Manyu Zhang Qi Liu Ruifang Li Wei Jiang Hongjin Zhao Wenwei Sheng Luming Xia Zengqiang Li Qing Sun Jingying Du Lei Lei Quan Wang A Novel Immunochromatographic Test Strip Using Lanthanide-Labeled Fluorescent Nanoparticles for the Serological Detection of <i>Toxoplasma gondii</i> in Dogs and Cats Pathogens lanthanide-labeled fluorescent nanoparticles fluorescence immunochromatographic strips seroprevalence <i>Toxoplasma gondii</i> Shanghai |
| title | A Novel Immunochromatographic Test Strip Using Lanthanide-Labeled Fluorescent Nanoparticles for the Serological Detection of <i>Toxoplasma gondii</i> in Dogs and Cats |
| title_full | A Novel Immunochromatographic Test Strip Using Lanthanide-Labeled Fluorescent Nanoparticles for the Serological Detection of <i>Toxoplasma gondii</i> in Dogs and Cats |
| title_fullStr | A Novel Immunochromatographic Test Strip Using Lanthanide-Labeled Fluorescent Nanoparticles for the Serological Detection of <i>Toxoplasma gondii</i> in Dogs and Cats |
| title_full_unstemmed | A Novel Immunochromatographic Test Strip Using Lanthanide-Labeled Fluorescent Nanoparticles for the Serological Detection of <i>Toxoplasma gondii</i> in Dogs and Cats |
| title_short | A Novel Immunochromatographic Test Strip Using Lanthanide-Labeled Fluorescent Nanoparticles for the Serological Detection of <i>Toxoplasma gondii</i> in Dogs and Cats |
| title_sort | novel immunochromatographic test strip using lanthanide labeled fluorescent nanoparticles for the serological detection of i toxoplasma gondii i in dogs and cats |
| topic | lanthanide-labeled fluorescent nanoparticles fluorescence immunochromatographic strips seroprevalence <i>Toxoplasma gondii</i> Shanghai |
| url | https://www.mdpi.com/2076-0817/13/11/931 |
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