Establishment and characterization of biofloc culture for marbled crayfish
Biofloc technology is an innovative aquaculture approach that enhances water quality and reduces waste through the development of beneficial microbial communities. Marbled crayfish (Procambarus virginalis) are a newly discovered freshwater crayfish species that are distinguished by asexual reproduct...
Saved in:
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-05-01
|
| Series: | Frontiers in Aquaculture |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/faquc.2025.1580560/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850148761262620672 |
|---|---|
| author | Vitor Coutinho Carneiro Vitor Coutinho Carneiro Ines Schäfer J. Jaime Diaz-Larrosa Elias Böhl Thomas Hahn Jule Hempelmann Geetha Venkatesh Sanjanaa Nagarajan Suki Roy Yi Long Ng Florian Böhl Susanne Zibek Frank Lyko |
| author_facet | Vitor Coutinho Carneiro Vitor Coutinho Carneiro Ines Schäfer J. Jaime Diaz-Larrosa Elias Böhl Thomas Hahn Jule Hempelmann Geetha Venkatesh Sanjanaa Nagarajan Suki Roy Yi Long Ng Florian Böhl Susanne Zibek Frank Lyko |
| author_sort | Vitor Coutinho Carneiro |
| collection | DOAJ |
| description | Biofloc technology is an innovative aquaculture approach that enhances water quality and reduces waste through the development of beneficial microbial communities. Marbled crayfish (Procambarus virginalis) are a newly discovered freshwater crayfish species that are distinguished by asexual reproduction, a genetically homogeneous all-female population structure and considerable robustness. These traits are attractive features for closed-system aquaculture production, which also mitigates the invasive risk associated with the species. Here we describe the establishment and characterization of biofloc culture for marbled crayfish. Juvenile crayfish raised in biofloc systems displayed robust growth with a six-fold weight increase over three months, comparable with other crustaceans under similar conditions. Metagenomic analysis revealed that biofloc communities consisted mostly of beneficial Gammaproteobacteria and Bacillariophyceae, with variations by environmental factors, such as temperature and light exposure. DNA methylation profiling identified systematic changes in response to biofloc environments, highlighting the potential of DNA methylation analysis for environmental biomarker identification. Finally, nutritional analysis showed that biofloc-grown crayfish produced meat with a highly favorable protein-to-fat ratio, while their shells retained considerable amounts of chitin, an important raw material for bioplastics production. Taken together, our study is the first to establish marbled crayfish biofloc culture and to characterize the interaction between the animals and the production system. |
| format | Article |
| id | doaj-art-dea08b2a833e409c8152c84fd3ba1725 |
| institution | OA Journals |
| issn | 2813-5334 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Aquaculture |
| spelling | doaj-art-dea08b2a833e409c8152c84fd3ba17252025-08-20T02:27:10ZengFrontiers Media S.A.Frontiers in Aquaculture2813-53342025-05-01410.3389/faquc.2025.15805601580560Establishment and characterization of biofloc culture for marbled crayfishVitor Coutinho Carneiro0Vitor Coutinho Carneiro1Ines Schäfer2J. Jaime Diaz-Larrosa3Elias Böhl4Thomas Hahn5Jule Hempelmann6Geetha Venkatesh7Sanjanaa Nagarajan8Suki Roy9Yi Long Ng10Florian Böhl11Susanne Zibek12Frank Lyko13Division of Epigenetics, DKFZ-ZMBH Alliance, German Cancer Research Center, Heidelberg, GermanyMerall Bioproducts GmbH, Heidelberg, GermanyDivision of Epigenetics, DKFZ-ZMBH Alliance, German Cancer Research Center, Heidelberg, GermanyDivision of Epigenetics, DKFZ-ZMBH Alliance, German Cancer Research Center, Heidelberg, GermanyDivision of Epigenetics, DKFZ-ZMBH Alliance, German Cancer Research Center, Heidelberg, GermanyFraunhofer IGB, Stuttgart, GermanyFraunhofer IGB, Stuttgart, GermanyDivision of Epigenetics, DKFZ-ZMBH Alliance, German Cancer Research Center, Heidelberg, GermanyEvonik (SEA) Pte Ltd, Asia Research Hub, Singapore, SingaporeEvonik (SEA) Pte Ltd, Asia Research Hub, Singapore, SingaporeSchool of Applied Science, Republic Polytechnic, Singapore, SingaporeCreavis, Evonik Operations GmbH, Hanau-Wolfgang, GermanyFraunhofer IGB, Stuttgart, GermanyDivision of Epigenetics, DKFZ-ZMBH Alliance, German Cancer Research Center, Heidelberg, GermanyBiofloc technology is an innovative aquaculture approach that enhances water quality and reduces waste through the development of beneficial microbial communities. Marbled crayfish (Procambarus virginalis) are a newly discovered freshwater crayfish species that are distinguished by asexual reproduction, a genetically homogeneous all-female population structure and considerable robustness. These traits are attractive features for closed-system aquaculture production, which also mitigates the invasive risk associated with the species. Here we describe the establishment and characterization of biofloc culture for marbled crayfish. Juvenile crayfish raised in biofloc systems displayed robust growth with a six-fold weight increase over three months, comparable with other crustaceans under similar conditions. Metagenomic analysis revealed that biofloc communities consisted mostly of beneficial Gammaproteobacteria and Bacillariophyceae, with variations by environmental factors, such as temperature and light exposure. DNA methylation profiling identified systematic changes in response to biofloc environments, highlighting the potential of DNA methylation analysis for environmental biomarker identification. Finally, nutritional analysis showed that biofloc-grown crayfish produced meat with a highly favorable protein-to-fat ratio, while their shells retained considerable amounts of chitin, an important raw material for bioplastics production. Taken together, our study is the first to establish marbled crayfish biofloc culture and to characterize the interaction between the animals and the production system.https://www.frontiersin.org/articles/10.3389/faquc.2025.1580560/fullbiofloc technologysustainabilityaquaculturemarbled crayfishepigeneticschitin |
| spellingShingle | Vitor Coutinho Carneiro Vitor Coutinho Carneiro Ines Schäfer J. Jaime Diaz-Larrosa Elias Böhl Thomas Hahn Jule Hempelmann Geetha Venkatesh Sanjanaa Nagarajan Suki Roy Yi Long Ng Florian Böhl Susanne Zibek Frank Lyko Establishment and characterization of biofloc culture for marbled crayfish Frontiers in Aquaculture biofloc technology sustainability aquaculture marbled crayfish epigenetics chitin |
| title | Establishment and characterization of biofloc culture for marbled crayfish |
| title_full | Establishment and characterization of biofloc culture for marbled crayfish |
| title_fullStr | Establishment and characterization of biofloc culture for marbled crayfish |
| title_full_unstemmed | Establishment and characterization of biofloc culture for marbled crayfish |
| title_short | Establishment and characterization of biofloc culture for marbled crayfish |
| title_sort | establishment and characterization of biofloc culture for marbled crayfish |
| topic | biofloc technology sustainability aquaculture marbled crayfish epigenetics chitin |
| url | https://www.frontiersin.org/articles/10.3389/faquc.2025.1580560/full |
| work_keys_str_mv | AT vitorcoutinhocarneiro establishmentandcharacterizationofbiofloccultureformarbledcrayfish AT vitorcoutinhocarneiro establishmentandcharacterizationofbiofloccultureformarbledcrayfish AT inesschafer establishmentandcharacterizationofbiofloccultureformarbledcrayfish AT jjaimediazlarrosa establishmentandcharacterizationofbiofloccultureformarbledcrayfish AT eliasbohl establishmentandcharacterizationofbiofloccultureformarbledcrayfish AT thomashahn establishmentandcharacterizationofbiofloccultureformarbledcrayfish AT julehempelmann establishmentandcharacterizationofbiofloccultureformarbledcrayfish AT geethavenkatesh establishmentandcharacterizationofbiofloccultureformarbledcrayfish AT sanjanaanagarajan establishmentandcharacterizationofbiofloccultureformarbledcrayfish AT sukiroy establishmentandcharacterizationofbiofloccultureformarbledcrayfish AT yilongng establishmentandcharacterizationofbiofloccultureformarbledcrayfish AT florianbohl establishmentandcharacterizationofbiofloccultureformarbledcrayfish AT susannezibek establishmentandcharacterizationofbiofloccultureformarbledcrayfish AT franklyko establishmentandcharacterizationofbiofloccultureformarbledcrayfish |