Antimicrobial Bioactivity and GC-MS Analysis of Different Extracts of Corchorus olitorius L Leaves
Background and Aim. Plants have been used for the treatment of infectious diseases among various communities worldwide with undeniable success. Corchorus olitorius L is used to treat various ailments including infections. However, there are limited data regarding its antimicrobial spectrum in Uganda...
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2022/3382302 |
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| author | Rebecca Nakaziba Sharon Bright Amanya Crispin Duncan Sesaazi Frederick Byarugaba Jasper Ogwal-Okeng Paul E. Alele |
| author_facet | Rebecca Nakaziba Sharon Bright Amanya Crispin Duncan Sesaazi Frederick Byarugaba Jasper Ogwal-Okeng Paul E. Alele |
| author_sort | Rebecca Nakaziba |
| collection | DOAJ |
| description | Background and Aim. Plants have been used for the treatment of infectious diseases among various communities worldwide with undeniable success. Corchorus olitorius L is used to treat various ailments including infections. However, there are limited data regarding its antimicrobial spectrum in Uganda. In the bid to bridge this gap, we investigated the antimicrobial bioactivity of Corchorus olitorius L against Candida albicans, Staphylococcus aureus, Streptococcus pneumoniae, Escherichia coli, and Mycobacteria tuberculosis. Methods. Corchorus olitorius L leaves were harvested, air-dried, and serially extracted using diethyl ether, methanol, and distilled water (aqueous). The crude extracts and their components separated by TLC were assayed for bioactivity against the organisms mentioned above using the agar well diffusion technique. Chloramphenicol was used as a positive control. Observation of a zone of inhibition indicated bioactivity. Finally, the components that displayed bioactivity were subjected to GC-MS analysis to establish their chemical composition using the MS library database. Results. Crude extracts of Corchorus olitorius L leaves and their TLC-separated components demonstrated bioactivity against Staphylococcus aureus (14 mm), Streptococcus pneumoniae (16 mm), and Escherichia coli (11 mm) but neither against Candida albicans nor Mycobacteria tuberculosis. However, the overall zones of inhibition were smaller compared to the positive control (≥18 mm). GC-MS analysis of the active components revealed the presence of methyl esters. Conclusion. Corchorus olitorius L is bioactive against both Gram-negative and Gram-positive bacteria but neither against fungi nor mycobacteria. The bioactivity is attributable to the presence of methyl esters. Since methyl esters already have proven bioactivity in some studies, they could be further studied and optimized for possible pharmaceutical use. Further, to provide a more comprehensive antimicrobial spectrum of Corchorus olitorius L in Uganda, purified active components could be investigated using a wider range of organisms. |
| format | Article |
| id | doaj-art-de97fe350c2d4155b685a744aa546a0b |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-de97fe350c2d4155b685a744aa546a0b2025-08-20T03:26:16ZengWileyThe Scientific World Journal1537-744X2022-01-01202210.1155/2022/3382302Antimicrobial Bioactivity and GC-MS Analysis of Different Extracts of Corchorus olitorius L LeavesRebecca Nakaziba0Sharon Bright Amanya1Crispin Duncan Sesaazi2Frederick Byarugaba3Jasper Ogwal-Okeng4Paul E. Alele5Department of Pharmacology and TherapeuticsDepartment of Microbiology and ImmunologyDepartment of Pharmaceutical SciencesDepartment of MicrobiologyDepartment of Pharmacology and TherapeuticsDepartment of Pharmacology and TherapeuticsBackground and Aim. Plants have been used for the treatment of infectious diseases among various communities worldwide with undeniable success. Corchorus olitorius L is used to treat various ailments including infections. However, there are limited data regarding its antimicrobial spectrum in Uganda. In the bid to bridge this gap, we investigated the antimicrobial bioactivity of Corchorus olitorius L against Candida albicans, Staphylococcus aureus, Streptococcus pneumoniae, Escherichia coli, and Mycobacteria tuberculosis. Methods. Corchorus olitorius L leaves were harvested, air-dried, and serially extracted using diethyl ether, methanol, and distilled water (aqueous). The crude extracts and their components separated by TLC were assayed for bioactivity against the organisms mentioned above using the agar well diffusion technique. Chloramphenicol was used as a positive control. Observation of a zone of inhibition indicated bioactivity. Finally, the components that displayed bioactivity were subjected to GC-MS analysis to establish their chemical composition using the MS library database. Results. Crude extracts of Corchorus olitorius L leaves and their TLC-separated components demonstrated bioactivity against Staphylococcus aureus (14 mm), Streptococcus pneumoniae (16 mm), and Escherichia coli (11 mm) but neither against Candida albicans nor Mycobacteria tuberculosis. However, the overall zones of inhibition were smaller compared to the positive control (≥18 mm). GC-MS analysis of the active components revealed the presence of methyl esters. Conclusion. Corchorus olitorius L is bioactive against both Gram-negative and Gram-positive bacteria but neither against fungi nor mycobacteria. The bioactivity is attributable to the presence of methyl esters. Since methyl esters already have proven bioactivity in some studies, they could be further studied and optimized for possible pharmaceutical use. Further, to provide a more comprehensive antimicrobial spectrum of Corchorus olitorius L in Uganda, purified active components could be investigated using a wider range of organisms.http://dx.doi.org/10.1155/2022/3382302 |
| spellingShingle | Rebecca Nakaziba Sharon Bright Amanya Crispin Duncan Sesaazi Frederick Byarugaba Jasper Ogwal-Okeng Paul E. Alele Antimicrobial Bioactivity and GC-MS Analysis of Different Extracts of Corchorus olitorius L Leaves The Scientific World Journal |
| title | Antimicrobial Bioactivity and GC-MS Analysis of Different Extracts of Corchorus olitorius L Leaves |
| title_full | Antimicrobial Bioactivity and GC-MS Analysis of Different Extracts of Corchorus olitorius L Leaves |
| title_fullStr | Antimicrobial Bioactivity and GC-MS Analysis of Different Extracts of Corchorus olitorius L Leaves |
| title_full_unstemmed | Antimicrobial Bioactivity and GC-MS Analysis of Different Extracts of Corchorus olitorius L Leaves |
| title_short | Antimicrobial Bioactivity and GC-MS Analysis of Different Extracts of Corchorus olitorius L Leaves |
| title_sort | antimicrobial bioactivity and gc ms analysis of different extracts of corchorus olitorius l leaves |
| url | http://dx.doi.org/10.1155/2022/3382302 |
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