Stem Cells for Bone Regeneration: From Cell-Based Therapies to Decellularised Engineered Extracellular Matrices

Currently, autologous bone grafting represents the clinical gold standard in orthopaedic surgery. In certain cases, however, alternative techniques are required. The clinical utility of stem and stromal cells has been demonstrated for the repair and regeneration of craniomaxillofacial and long bone...

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Main Authors: James N. Fisher, Giuseppe M. Peretti, Celeste Scotti
Format: Article
Language:English
Published: Wiley 2016-01-01
Series:Stem Cells International
Online Access:http://dx.doi.org/10.1155/2016/9352598
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author James N. Fisher
Giuseppe M. Peretti
Celeste Scotti
author_facet James N. Fisher
Giuseppe M. Peretti
Celeste Scotti
author_sort James N. Fisher
collection DOAJ
description Currently, autologous bone grafting represents the clinical gold standard in orthopaedic surgery. In certain cases, however, alternative techniques are required. The clinical utility of stem and stromal cells has been demonstrated for the repair and regeneration of craniomaxillofacial and long bone defects although clinical adoption of bone tissue engineering protocols has been very limited. Initial tissue engineering studies focused on the bone marrow as a source of cells for bone regeneration, and while a number of promising results continue to emerge, limitations to this technique have prompted the exploration of alternative cell sources, including adipose and muscle tissue. In this review paper we discuss the advantages and disadvantages of cell sources with a focus on adipose tissue and the bone marrow. Additionally, we highlight the relatively recent paradigm of developmental engineering, which promotes the recapitulation of naturally occurring developmental processes to allow the implant to optimally respond to endogenous cues. Finally we examine efforts to apply lessons from studies into different cell sources and developmental approaches to stimulate bone growth by use of decellularised hypertrophic cartilage templates.
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spelling doaj-art-de979938b06d4febb3c8e5bd2bf49b282025-08-20T03:26:16ZengWileyStem Cells International1687-966X1687-96782016-01-01201610.1155/2016/93525989352598Stem Cells for Bone Regeneration: From Cell-Based Therapies to Decellularised Engineered Extracellular MatricesJames N. Fisher0Giuseppe M. Peretti1Celeste Scotti2IRCCS Istituto Ortopedico Galeazzi, 20161 Milan, ItalyIRCCS Istituto Ortopedico Galeazzi, 20161 Milan, ItalyIRCCS Istituto Ortopedico Galeazzi, 20161 Milan, ItalyCurrently, autologous bone grafting represents the clinical gold standard in orthopaedic surgery. In certain cases, however, alternative techniques are required. The clinical utility of stem and stromal cells has been demonstrated for the repair and regeneration of craniomaxillofacial and long bone defects although clinical adoption of bone tissue engineering protocols has been very limited. Initial tissue engineering studies focused on the bone marrow as a source of cells for bone regeneration, and while a number of promising results continue to emerge, limitations to this technique have prompted the exploration of alternative cell sources, including adipose and muscle tissue. In this review paper we discuss the advantages and disadvantages of cell sources with a focus on adipose tissue and the bone marrow. Additionally, we highlight the relatively recent paradigm of developmental engineering, which promotes the recapitulation of naturally occurring developmental processes to allow the implant to optimally respond to endogenous cues. Finally we examine efforts to apply lessons from studies into different cell sources and developmental approaches to stimulate bone growth by use of decellularised hypertrophic cartilage templates.http://dx.doi.org/10.1155/2016/9352598
spellingShingle James N. Fisher
Giuseppe M. Peretti
Celeste Scotti
Stem Cells for Bone Regeneration: From Cell-Based Therapies to Decellularised Engineered Extracellular Matrices
Stem Cells International
title Stem Cells for Bone Regeneration: From Cell-Based Therapies to Decellularised Engineered Extracellular Matrices
title_full Stem Cells for Bone Regeneration: From Cell-Based Therapies to Decellularised Engineered Extracellular Matrices
title_fullStr Stem Cells for Bone Regeneration: From Cell-Based Therapies to Decellularised Engineered Extracellular Matrices
title_full_unstemmed Stem Cells for Bone Regeneration: From Cell-Based Therapies to Decellularised Engineered Extracellular Matrices
title_short Stem Cells for Bone Regeneration: From Cell-Based Therapies to Decellularised Engineered Extracellular Matrices
title_sort stem cells for bone regeneration from cell based therapies to decellularised engineered extracellular matrices
url http://dx.doi.org/10.1155/2016/9352598
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AT celestescotti stemcellsforboneregenerationfromcellbasedtherapiestodecellularisedengineeredextracellularmatrices