Feasibility of extracting usable DNA from blood samples stored up to 21 years in the DiPiS study
Abstract This study assesses the feasibility of extracting high-quality DNA from blood samples stored at – 20 °C for up to 21 years under suboptimal conditions. It addresses sample mishandling in research, where many samples lack proper biobank protocols. Prior studies focused on short-term storage...
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Nature Portfolio
2025-07-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-025-08257-y |
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| author | Agnes Andersson Svärd Ellen Viberg India von Platen Ida Jönsson Markus Lundgren on behalf of the DiPiS study group |
| author_facet | Agnes Andersson Svärd Ellen Viberg India von Platen Ida Jönsson Markus Lundgren on behalf of the DiPiS study group |
| author_sort | Agnes Andersson Svärd |
| collection | DOAJ |
| description | Abstract This study assesses the feasibility of extracting high-quality DNA from blood samples stored at – 20 °C for up to 21 years under suboptimal conditions. It addresses sample mishandling in research, where many samples lack proper biobank protocols. Prior studies focused on short-term storage and controlled conditions, highlighting the negative effects of freeze–thaw cycles. This study evaluates whether DNA from long-term stored samples under suboptimal conditions can still meet quality standards for research purposes. Genomic DNA was extracted from 1012 capillary blood samples from the Diabetes Prediction in Skåne study. Samples were stored at – 20 °C for 7–21 years, and DNA was isolated using QIAamp DNA Blood Mini kits. DNA quantity, purity, and quality were analysed using spectrophotometry and automated electrophoresis. Overall, 75.7% of samples met quality standards for DNA quantity (≥ 20 ng/µL) and purity (A260/280 ratio 1.7–1.9), with the highest proportion in 12-year samples (83.5%). DNA quality was further assessed in 270 samples, where 57.8% had a DNA Integrity Number (DIN) of 7 or higher. This study suggests that historical blood samples stored under suboptmal conditions can still be viable for modern genomic analyses. |
| format | Article |
| id | doaj-art-de8bf0d359f74896ab54012748d38f82 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-de8bf0d359f74896ab54012748d38f822025-08-20T03:42:28ZengNature PortfolioScientific Reports2045-23222025-07-0115111110.1038/s41598-025-08257-yFeasibility of extracting usable DNA from blood samples stored up to 21 years in the DiPiS studyAgnes Andersson Svärd0Ellen Viberg1India von Platen2Ida Jönsson3Markus Lundgren4on behalf of the DiPiS study groupDepartment of Clinical Sciences, Lund UniversityDepartment of Clinical Sciences, Lund UniversityDepartment of Clinical Sciences, Lund UniversityDepartment of Clinical Sciences, Lund UniversityDepartment of Clinical Sciences, Lund UniversityAbstract This study assesses the feasibility of extracting high-quality DNA from blood samples stored at – 20 °C for up to 21 years under suboptimal conditions. It addresses sample mishandling in research, where many samples lack proper biobank protocols. Prior studies focused on short-term storage and controlled conditions, highlighting the negative effects of freeze–thaw cycles. This study evaluates whether DNA from long-term stored samples under suboptimal conditions can still meet quality standards for research purposes. Genomic DNA was extracted from 1012 capillary blood samples from the Diabetes Prediction in Skåne study. Samples were stored at – 20 °C for 7–21 years, and DNA was isolated using QIAamp DNA Blood Mini kits. DNA quantity, purity, and quality were analysed using spectrophotometry and automated electrophoresis. Overall, 75.7% of samples met quality standards for DNA quantity (≥ 20 ng/µL) and purity (A260/280 ratio 1.7–1.9), with the highest proportion in 12-year samples (83.5%). DNA quality was further assessed in 270 samples, where 57.8% had a DNA Integrity Number (DIN) of 7 or higher. This study suggests that historical blood samples stored under suboptmal conditions can still be viable for modern genomic analyses.https://doi.org/10.1038/s41598-025-08257-yBlood sampleLong-term sample storageDNA isolationDNA concentrationDNA quality |
| spellingShingle | Agnes Andersson Svärd Ellen Viberg India von Platen Ida Jönsson Markus Lundgren on behalf of the DiPiS study group Feasibility of extracting usable DNA from blood samples stored up to 21 years in the DiPiS study Scientific Reports Blood sample Long-term sample storage DNA isolation DNA concentration DNA quality |
| title | Feasibility of extracting usable DNA from blood samples stored up to 21 years in the DiPiS study |
| title_full | Feasibility of extracting usable DNA from blood samples stored up to 21 years in the DiPiS study |
| title_fullStr | Feasibility of extracting usable DNA from blood samples stored up to 21 years in the DiPiS study |
| title_full_unstemmed | Feasibility of extracting usable DNA from blood samples stored up to 21 years in the DiPiS study |
| title_short | Feasibility of extracting usable DNA from blood samples stored up to 21 years in the DiPiS study |
| title_sort | feasibility of extracting usable dna from blood samples stored up to 21 years in the dipis study |
| topic | Blood sample Long-term sample storage DNA isolation DNA concentration DNA quality |
| url | https://doi.org/10.1038/s41598-025-08257-y |
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