The Interplay Between Melatonin and Nitric Oxide: Mechanisms and Implications in Stroke Pathophysiology

This work reviews the complex interplay between melatonin and nitric oxide (NO) in the central nervous system (CNS), with a detailed focus on its involvement in stroke pathophysiology. Melatonin, a neurohormone with potent antioxidant, anti-inflammatory, and neuroprotective properties, and NO, a gas...

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Main Authors: Santos Blanco, María del Mar Muñoz-Gallardo, Raquel Hernández, María Ángeles Peinado
Format: Article
Language:English
Published: MDPI AG 2025-06-01
Series:Antioxidants
Subjects:
Online Access:https://www.mdpi.com/2076-3921/14/6/724
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author Santos Blanco
María del Mar Muñoz-Gallardo
Raquel Hernández
María Ángeles Peinado
author_facet Santos Blanco
María del Mar Muñoz-Gallardo
Raquel Hernández
María Ángeles Peinado
author_sort Santos Blanco
collection DOAJ
description This work reviews the complex interplay between melatonin and nitric oxide (NO) in the central nervous system (CNS), with a detailed focus on its involvement in stroke pathophysiology. Melatonin, a neurohormone with potent antioxidant, anti-inflammatory, and neuroprotective properties, and NO, a gaseous signaling molecule with diverse roles, interact crucially. In the context of ischemic stroke, NO exhibits a dual role: it can be neuroprotective (primarily via endothelial nitric oxide synthase (eNOS)) or neurotoxic (especially through inducible nitric oxide synthase (iNOS) and neuronal nitric oxide synthase (nNOS), contributing to the formation of damaging peroxynitrite (ONOO<sup>−</sup>)). Melatonin has consistently demonstrated neuroprotective effects in animal models of stroke. Its key mechanisms related to NO include (1) differential modulation of nitric oxide synthase isoforms, suppressing detrimental iNOS expression/activity while often preserving or enhancing beneficial eNOS; (2) direct scavenging of NO and, critically, highly reactive peroxynitrite, thereby attenuating nitrosative stress; (3) reduction in neuroinflammation, partly by promoting M2 (anti-inflammatory) microglia polarization; and (4) mitochondrial protection and decreased apoptosis. These multifaceted actions of melatonin contribute to reduced infarct volume and improved functional outcomes, underscoring its considerable therapeutic potential for ischemic stroke through the favorable modulation of the melatonin–NO axis.
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spelling doaj-art-de7e3e753ea0440193ed854ffbb7f7962025-08-20T03:26:09ZengMDPI AGAntioxidants2076-39212025-06-0114672410.3390/antiox14060724The Interplay Between Melatonin and Nitric Oxide: Mechanisms and Implications in Stroke PathophysiologySantos Blanco0María del Mar Muñoz-Gallardo1Raquel Hernández2María Ángeles Peinado3Department of Experimental Biology, University of Jaén, 23007 Jaén, SpainDepartment of Experimental Biology, University of Jaén, 23007 Jaén, SpainDepartment of Experimental Biology, University of Jaén, 23007 Jaén, SpainDepartment of Experimental Biology, University of Jaén, 23007 Jaén, SpainThis work reviews the complex interplay between melatonin and nitric oxide (NO) in the central nervous system (CNS), with a detailed focus on its involvement in stroke pathophysiology. Melatonin, a neurohormone with potent antioxidant, anti-inflammatory, and neuroprotective properties, and NO, a gaseous signaling molecule with diverse roles, interact crucially. In the context of ischemic stroke, NO exhibits a dual role: it can be neuroprotective (primarily via endothelial nitric oxide synthase (eNOS)) or neurotoxic (especially through inducible nitric oxide synthase (iNOS) and neuronal nitric oxide synthase (nNOS), contributing to the formation of damaging peroxynitrite (ONOO<sup>−</sup>)). Melatonin has consistently demonstrated neuroprotective effects in animal models of stroke. Its key mechanisms related to NO include (1) differential modulation of nitric oxide synthase isoforms, suppressing detrimental iNOS expression/activity while often preserving or enhancing beneficial eNOS; (2) direct scavenging of NO and, critically, highly reactive peroxynitrite, thereby attenuating nitrosative stress; (3) reduction in neuroinflammation, partly by promoting M2 (anti-inflammatory) microglia polarization; and (4) mitochondrial protection and decreased apoptosis. These multifaceted actions of melatonin contribute to reduced infarct volume and improved functional outcomes, underscoring its considerable therapeutic potential for ischemic stroke through the favorable modulation of the melatonin–NO axis.https://www.mdpi.com/2076-3921/14/6/724melatoninnitric oxidestrokeCNSischemiamolecular mechanisms
spellingShingle Santos Blanco
María del Mar Muñoz-Gallardo
Raquel Hernández
María Ángeles Peinado
The Interplay Between Melatonin and Nitric Oxide: Mechanisms and Implications in Stroke Pathophysiology
Antioxidants
melatonin
nitric oxide
stroke
CNS
ischemia
molecular mechanisms
title The Interplay Between Melatonin and Nitric Oxide: Mechanisms and Implications in Stroke Pathophysiology
title_full The Interplay Between Melatonin and Nitric Oxide: Mechanisms and Implications in Stroke Pathophysiology
title_fullStr The Interplay Between Melatonin and Nitric Oxide: Mechanisms and Implications in Stroke Pathophysiology
title_full_unstemmed The Interplay Between Melatonin and Nitric Oxide: Mechanisms and Implications in Stroke Pathophysiology
title_short The Interplay Between Melatonin and Nitric Oxide: Mechanisms and Implications in Stroke Pathophysiology
title_sort interplay between melatonin and nitric oxide mechanisms and implications in stroke pathophysiology
topic melatonin
nitric oxide
stroke
CNS
ischemia
molecular mechanisms
url https://www.mdpi.com/2076-3921/14/6/724
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