Stability and functionality of bovine lactoferrin powder after 9 years of storage
Bovine lactoferrin (bLF) is a multifunctional protein widely used in food industries. Most bLF products are delivered in a powder form; however, their stability remains unclear. Herein, freeze-dried bLF powders were stored at 4 °C and 40 % relative humidity (RH) for 9 years since 2016. After the lon...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-01-01
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| Series: | Current Research in Food Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S266592712500067X |
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| author | Yin Hu Shubo Luo Yuhong Jiang Jie Lin Baoguo Xu Zhi-Hong Zhang Benu Adhikari Tiantian Xu Bo Wang |
| author_facet | Yin Hu Shubo Luo Yuhong Jiang Jie Lin Baoguo Xu Zhi-Hong Zhang Benu Adhikari Tiantian Xu Bo Wang |
| author_sort | Yin Hu |
| collection | DOAJ |
| description | Bovine lactoferrin (bLF) is a multifunctional protein widely used in food industries. Most bLF products are delivered in a powder form; however, their stability remains unclear. Herein, freeze-dried bLF powders were stored at 4 °C and 40 % relative humidity (RH) for 9 years since 2016. After the long-term storage, their functional properties, including antibacterial ability, antioxidant ability and iron-binding ability, were determined and compared with those of eight commercial LF powders. The bLF powder stored for 9 years demonstrated comparable physicochemical and functional properties with those of commercial LF powders (e.g. >93 % water solubility, >100 mg/100 g iron-binding ability, and >1.7 logCFU/mL bacterial growth reduction against Salmonella enteritidis). The haemolysis test indicated that the bLF stored for 9 years exhibited good biocompatibility at a concentration of <5 mg/mL. Therefore, bLF powders can be stored for extremely long periods (>9 years) with minimal side effects. These findings can expand the utilisation of lactoferrin to many specific cases such as voyage and aerospace foods. |
| format | Article |
| id | doaj-art-de791c2cf6ba4c33b705d2b5d77d002e |
| institution | DOAJ |
| issn | 2665-9271 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Current Research in Food Science |
| spelling | doaj-art-de791c2cf6ba4c33b705d2b5d77d002e2025-08-20T03:20:05ZengElsevierCurrent Research in Food Science2665-92712025-01-011010103610.1016/j.crfs.2025.101036Stability and functionality of bovine lactoferrin powder after 9 years of storageYin Hu0Shubo Luo1Yuhong Jiang2Jie Lin3Baoguo Xu4Zhi-Hong Zhang5Benu Adhikari6Tiantian Xu7Bo Wang8School of Food and Biological Engineering, Jiangsu University, 212013, Zhenjiang, Jiangsu, ChinaHeilongjiang Feihe Dairy Co., Ltd, 164800, Harbin, Heilongjiang, ChinaNanjing Bestzyme Bio-Engineering Co., Ltd, 211100, Nanjing, Jiangsu, ChinaNanjing Bestzyme Bio-Engineering Co., Ltd, 211100, Nanjing, Jiangsu, ChinaSchool of Food and Biological Engineering, Jiangsu University, 212013, Zhenjiang, Jiangsu, ChinaSchool of Food and Biological Engineering, Jiangsu University, 212013, Zhenjiang, Jiangsu, ChinaSchool of Science, RMIT University, VIC, 3083, Melbourne, Victoria, AustraliaLaboratory Animal Research Center, Jiangsu University, 212013, Zhenjiang, Jiangsu, China; Corresponding author.School of Food and Biological Engineering, Jiangsu University, 212013, Zhenjiang, Jiangsu, China; Corresponding author.Bovine lactoferrin (bLF) is a multifunctional protein widely used in food industries. Most bLF products are delivered in a powder form; however, their stability remains unclear. Herein, freeze-dried bLF powders were stored at 4 °C and 40 % relative humidity (RH) for 9 years since 2016. After the long-term storage, their functional properties, including antibacterial ability, antioxidant ability and iron-binding ability, were determined and compared with those of eight commercial LF powders. The bLF powder stored for 9 years demonstrated comparable physicochemical and functional properties with those of commercial LF powders (e.g. >93 % water solubility, >100 mg/100 g iron-binding ability, and >1.7 logCFU/mL bacterial growth reduction against Salmonella enteritidis). The haemolysis test indicated that the bLF stored for 9 years exhibited good biocompatibility at a concentration of <5 mg/mL. Therefore, bLF powders can be stored for extremely long periods (>9 years) with minimal side effects. These findings can expand the utilisation of lactoferrin to many specific cases such as voyage and aerospace foods.http://www.sciencedirect.com/science/article/pii/S266592712500067XLactoferrinLong-term storageAntibacterial activityAntioxidant activity |
| spellingShingle | Yin Hu Shubo Luo Yuhong Jiang Jie Lin Baoguo Xu Zhi-Hong Zhang Benu Adhikari Tiantian Xu Bo Wang Stability and functionality of bovine lactoferrin powder after 9 years of storage Current Research in Food Science Lactoferrin Long-term storage Antibacterial activity Antioxidant activity |
| title | Stability and functionality of bovine lactoferrin powder after 9 years of storage |
| title_full | Stability and functionality of bovine lactoferrin powder after 9 years of storage |
| title_fullStr | Stability and functionality of bovine lactoferrin powder after 9 years of storage |
| title_full_unstemmed | Stability and functionality of bovine lactoferrin powder after 9 years of storage |
| title_short | Stability and functionality of bovine lactoferrin powder after 9 years of storage |
| title_sort | stability and functionality of bovine lactoferrin powder after 9 years of storage |
| topic | Lactoferrin Long-term storage Antibacterial activity Antioxidant activity |
| url | http://www.sciencedirect.com/science/article/pii/S266592712500067X |
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