Apiospora arundinis, a panoply of carbohydrate-active enzymes and secondary metabolites

Apiospora arundinis, a panoply of carbohydrate-active enzymes and secondary metabolites

Abstract The Apiospora genus comprises filamentous fungi with promising potential, though its full capabilities remain undiscovered. In this study, we present the first genome assembly of an Apiospora arundinis isolate, demonstrating a highly complete and contiguous assembly estimated to 48.8 Mb, wi...

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Main Authors: Trine Sørensen, Celine Petersen, Asmus T. Muurmann, Johan V. Christiansen, Mathias L. Brundtø, Christina K. Overgaard, Anders T. Boysen, Rasmus D. Wollenberg, Thomas O. Larsen, Jens L. Sørensen, Kåre L. Nielsen, Teis E. Sondergaard
Format: Article
Language:English
Published: BMC 2024-04-01
Series:IMA Fungus
Subjects:
Oxford Nanopore sequencing
Apiospora
Arthrinium
CAZymes
Secondary metabolites
Online Access:https://doi.org/10.1186/s43008-024-00141-0
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Summary:Abstract The Apiospora genus comprises filamentous fungi with promising potential, though its full capabilities remain undiscovered. In this study, we present the first genome assembly of an Apiospora arundinis isolate, demonstrating a highly complete and contiguous assembly estimated to 48.8 Mb, with an N99 of 3.0 Mb. Our analysis predicted a total of 15,725 genes, with functional annotations for 13,619 of them, revealing a fungus capable of producing very high amounts of carbohydrate-active enzymes (CAZymes) and secondary metabolites. Through transcriptomic analysis, we observed differential gene expression in response to varying growth media, with several genes related to carbohydrate metabolism showing significant upregulation when the fungus was cultivated on a hay-based medium. Finally, our metabolomic analysis unveiled a fungus capable of producing a diverse array of metabolites.
ISSN:2210-6359

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