Gestational zearalenone causes fetal intrauterine growth restriction partially through deriving ROS-Drp1 mediated placental PANoptosis

The ubiquity of zearalenone (ZEA) in cereal-based products and the aquatic environment raises growing concerns about health problems to humans and animals. Here, we explored the mechanism by which ZEA exposure during pregnancy induced fetal growth restriction (FGR). Interestingly, both fetal weights...

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Main Authors: Jie Ru Liu, Fang Nan Wu, Shuai Lin, Chen Chen, Yan Liu, Jing Wen Fan, Qiang Hong, Yuan Hua Chen
Format: Article
Language:English
Published: Elsevier 2025-09-01
Series:Ecotoxicology and Environmental Safety
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Online Access:http://www.sciencedirect.com/science/article/pii/S0147651325009819
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author Jie Ru Liu
Fang Nan Wu
Shuai Lin
Chen Chen
Yan Liu
Jing Wen Fan
Qiang Hong
Yuan Hua Chen
author_facet Jie Ru Liu
Fang Nan Wu
Shuai Lin
Chen Chen
Yan Liu
Jing Wen Fan
Qiang Hong
Yuan Hua Chen
author_sort Jie Ru Liu
collection DOAJ
description The ubiquity of zearalenone (ZEA) in cereal-based products and the aquatic environment raises growing concerns about health problems to humans and animals. Here, we explored the mechanism by which ZEA exposure during pregnancy induced fetal growth restriction (FGR). Interestingly, both fetal weights and crown-rump length were significant decreases when dams were administrated with ZEA. Consistently, the incidence of FGR is significantly increased in ZEA group in a dose-dependent manner. Moreover, mean placental weight and diameter was significantly reduced in ZEA group, suggesting that poor placental development may be involved in ZEA-induced FGR. The genome-wide expression profiles of mouse placentas were significantly different between two groups by RNA-sequencing. GO and KEGG analysis indicated significant enrichment of these differentially expressed genes in mitochondrial apoptotic signaling pathway, inflammatory cell apoptotic process, necroptosis, and regulation of mitochondrial membrane potential. Further study showed that mitochondrial quality control disorder and PANoptosis plays an important role in ZEA-induced poor placental development. Mdivi-1, an inhibitor of Drp-1, attenuated ZEA-induced mitochondrial quality control disorder and PANoptosis in mouse placentas and human placental trophoblasts. N-acetylcysteine (NAC), an antioxidant, abolished ZEA-induced mitochondrial quality control disorder and PANoptosis in mouse placentas and human placental trophoblasts. Importantly, Mdivi-1 and NAC rescued gestational ZEA exposure-induced poor placental development and FGR in mice. Our results indicate that ZEA exposure during pregnancy caused poor placental development and subsequently FGR may be via deriving ROS-Drp1 mediated placental PANoptosis.
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spelling doaj-art-de65e4178555431389bdd0de72e40d832025-08-20T03:36:10ZengElsevierEcotoxicology and Environmental Safety0147-65132025-09-0130211863610.1016/j.ecoenv.2025.118636Gestational zearalenone causes fetal intrauterine growth restriction partially through deriving ROS-Drp1 mediated placental PANoptosisJie Ru Liu0Fang Nan Wu1Shuai Lin2Chen Chen3Yan Liu4Jing Wen Fan5Qiang Hong6Yuan Hua Chen7School of Basic Medical Sciences, Anhui Medical University, Hefei 230032, ChinaSchool of Basic Medical Sciences, Anhui Medical University, Hefei 230032, ChinaSchool of Basic Medical Sciences, Anhui Medical University, Hefei 230032, ChinaSchool of Basic Medical Sciences, Anhui Medical University, Hefei 230032, ChinaSchool of Basic Medical Sciences, Anhui Medical University, Hefei 230032, ChinaSchool of Basic Medical Sciences, Anhui Medical University, Hefei 230032, ChinaSchool of Basic Medical Sciences, Anhui Medical University, Hefei 230032, ChinaSchool of Basic Medical Sciences, Anhui Medical University, Hefei 230032, China; Key Laboratory of Environmental Toxicology of Anhui Higher Education Institutes, Anhui Medical University, Hefei 230032, China; Correspondence to: Anhui Medical University, Hefei 230032, China.The ubiquity of zearalenone (ZEA) in cereal-based products and the aquatic environment raises growing concerns about health problems to humans and animals. Here, we explored the mechanism by which ZEA exposure during pregnancy induced fetal growth restriction (FGR). Interestingly, both fetal weights and crown-rump length were significant decreases when dams were administrated with ZEA. Consistently, the incidence of FGR is significantly increased in ZEA group in a dose-dependent manner. Moreover, mean placental weight and diameter was significantly reduced in ZEA group, suggesting that poor placental development may be involved in ZEA-induced FGR. The genome-wide expression profiles of mouse placentas were significantly different between two groups by RNA-sequencing. GO and KEGG analysis indicated significant enrichment of these differentially expressed genes in mitochondrial apoptotic signaling pathway, inflammatory cell apoptotic process, necroptosis, and regulation of mitochondrial membrane potential. Further study showed that mitochondrial quality control disorder and PANoptosis plays an important role in ZEA-induced poor placental development. Mdivi-1, an inhibitor of Drp-1, attenuated ZEA-induced mitochondrial quality control disorder and PANoptosis in mouse placentas and human placental trophoblasts. N-acetylcysteine (NAC), an antioxidant, abolished ZEA-induced mitochondrial quality control disorder and PANoptosis in mouse placentas and human placental trophoblasts. Importantly, Mdivi-1 and NAC rescued gestational ZEA exposure-induced poor placental development and FGR in mice. Our results indicate that ZEA exposure during pregnancy caused poor placental development and subsequently FGR may be via deriving ROS-Drp1 mediated placental PANoptosis.http://www.sciencedirect.com/science/article/pii/S0147651325009819ZearalenoneFetal growth restrictionPANoptosisMitochondrial quality control disorderPlacenta
spellingShingle Jie Ru Liu
Fang Nan Wu
Shuai Lin
Chen Chen
Yan Liu
Jing Wen Fan
Qiang Hong
Yuan Hua Chen
Gestational zearalenone causes fetal intrauterine growth restriction partially through deriving ROS-Drp1 mediated placental PANoptosis
Ecotoxicology and Environmental Safety
Zearalenone
Fetal growth restriction
PANoptosis
Mitochondrial quality control disorder
Placenta
title Gestational zearalenone causes fetal intrauterine growth restriction partially through deriving ROS-Drp1 mediated placental PANoptosis
title_full Gestational zearalenone causes fetal intrauterine growth restriction partially through deriving ROS-Drp1 mediated placental PANoptosis
title_fullStr Gestational zearalenone causes fetal intrauterine growth restriction partially through deriving ROS-Drp1 mediated placental PANoptosis
title_full_unstemmed Gestational zearalenone causes fetal intrauterine growth restriction partially through deriving ROS-Drp1 mediated placental PANoptosis
title_short Gestational zearalenone causes fetal intrauterine growth restriction partially through deriving ROS-Drp1 mediated placental PANoptosis
title_sort gestational zearalenone causes fetal intrauterine growth restriction partially through deriving ros drp1 mediated placental panoptosis
topic Zearalenone
Fetal growth restriction
PANoptosis
Mitochondrial quality control disorder
Placenta
url http://www.sciencedirect.com/science/article/pii/S0147651325009819
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