Dissecting the genetic architecture of sunflower disc diameter using genome‐wide association study
Abstract Sunflower (Helianthus annuus L.) plays an essential role in meeting the demand for edible oil worldwide. The yield of sunflower seeds encompasses several component traits, including the disc diameter. Over three consecutive years, 2019, 2020, and 2022, we assessed phenotypic variation in di...
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| Format: | Article |
| Language: | English |
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Wiley
2024-10-01
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| Series: | Plant Direct |
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| Online Access: | https://doi.org/10.1002/pld3.70010 |
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| author | Yavuz Delen Ravi V. Mural Semra Palali‐Delen Gen Xu James C. Schnable Ismail Dweikat Jinliang Yang |
| author_facet | Yavuz Delen Ravi V. Mural Semra Palali‐Delen Gen Xu James C. Schnable Ismail Dweikat Jinliang Yang |
| author_sort | Yavuz Delen |
| collection | DOAJ |
| description | Abstract Sunflower (Helianthus annuus L.) plays an essential role in meeting the demand for edible oil worldwide. The yield of sunflower seeds encompasses several component traits, including the disc diameter. Over three consecutive years, 2019, 2020, and 2022, we assessed phenotypic variation in disc diameter across a diverse set of sunflower accessions (N = 342) in replicated field trials. Upon aggregating the phenotypic data from multiple years, we estimated the broad sense heritability (H2) of the disc diameter trait to be 0.88. A subset of N = 274 accessions was genotyped by using the tunable genotyping‐by‐sequencing (tGBS) method, resulting in 226,779 high‐quality SNPs. Using these SNPs and the disc diameter phenotype, we conducted a genome‐wide association study (GWAS) employing two statistical approaches: the mixed linear model (MLM) and the fixed and random model circulating probability unification (farmCPU). The MLM and farmCPU GWAS approaches identified 106 and 8 significant SNPs located close to 53 and 21 genes, respectively. The MLM analysis identified two significant peaks: a prominent signal on chromosome 10 and a relatively weaker signal on chromosome 16, both of which were also detected by farmCPU. The genetic loci associated with disc diameter, as well as the related candidate genes, present promising avenues for further functional validation and serve as a basis for sunflower oil yield improvement. |
| format | Article |
| id | doaj-art-de4c8a04e1a643eaa313b26db9cf8146 |
| institution | Kabale University |
| issn | 2475-4455 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Wiley |
| record_format | Article |
| series | Plant Direct |
| spelling | doaj-art-de4c8a04e1a643eaa313b26db9cf81462025-02-04T08:31:56ZengWileyPlant Direct2475-44552024-10-01810n/an/a10.1002/pld3.70010Dissecting the genetic architecture of sunflower disc diameter using genome‐wide association studyYavuz Delen0Ravi V. Mural1Semra Palali‐Delen2Gen Xu3James C. Schnable4Ismail Dweikat5Jinliang Yang6Department of Agronomy and Horticulture University of Nebraska‐Lincoln Lincoln NE USADepartment of Agronomy, Horticulture and Plant Science South Dakota State University Brookings SD USADepartment of Agronomy and Horticulture University of Nebraska‐Lincoln Lincoln NE USADepartment of Agronomy and Horticulture University of Nebraska‐Lincoln Lincoln NE USADepartment of Agronomy and Horticulture University of Nebraska‐Lincoln Lincoln NE USADepartment of Agronomy and Horticulture University of Nebraska‐Lincoln Lincoln NE USADepartment of Agronomy and Horticulture University of Nebraska‐Lincoln Lincoln NE USAAbstract Sunflower (Helianthus annuus L.) plays an essential role in meeting the demand for edible oil worldwide. The yield of sunflower seeds encompasses several component traits, including the disc diameter. Over three consecutive years, 2019, 2020, and 2022, we assessed phenotypic variation in disc diameter across a diverse set of sunflower accessions (N = 342) in replicated field trials. Upon aggregating the phenotypic data from multiple years, we estimated the broad sense heritability (H2) of the disc diameter trait to be 0.88. A subset of N = 274 accessions was genotyped by using the tunable genotyping‐by‐sequencing (tGBS) method, resulting in 226,779 high‐quality SNPs. Using these SNPs and the disc diameter phenotype, we conducted a genome‐wide association study (GWAS) employing two statistical approaches: the mixed linear model (MLM) and the fixed and random model circulating probability unification (farmCPU). The MLM and farmCPU GWAS approaches identified 106 and 8 significant SNPs located close to 53 and 21 genes, respectively. The MLM analysis identified two significant peaks: a prominent signal on chromosome 10 and a relatively weaker signal on chromosome 16, both of which were also detected by farmCPU. The genetic loci associated with disc diameter, as well as the related candidate genes, present promising avenues for further functional validation and serve as a basis for sunflower oil yield improvement.https://doi.org/10.1002/pld3.70010disc diameterGWAShelianthussunflower |
| spellingShingle | Yavuz Delen Ravi V. Mural Semra Palali‐Delen Gen Xu James C. Schnable Ismail Dweikat Jinliang Yang Dissecting the genetic architecture of sunflower disc diameter using genome‐wide association study Plant Direct disc diameter GWAS helianthus sunflower |
| title | Dissecting the genetic architecture of sunflower disc diameter using genome‐wide association study |
| title_full | Dissecting the genetic architecture of sunflower disc diameter using genome‐wide association study |
| title_fullStr | Dissecting the genetic architecture of sunflower disc diameter using genome‐wide association study |
| title_full_unstemmed | Dissecting the genetic architecture of sunflower disc diameter using genome‐wide association study |
| title_short | Dissecting the genetic architecture of sunflower disc diameter using genome‐wide association study |
| title_sort | dissecting the genetic architecture of sunflower disc diameter using genome wide association study |
| topic | disc diameter GWAS helianthus sunflower |
| url | https://doi.org/10.1002/pld3.70010 |
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