Dynamic Variation of Secondary Metabolites from <i>Polygonatum cyrtonema</i> Hua Rhizomes During Repeated Steaming–Drying Processes

Polygonati Rhizoma, widely used as a traditional functional food and herbal medicine, is well known for its health-promoting activities after the process of “nine cycles of steaming-drying”. Based on UPLC-MS/MS, 1369 secondary metabolites were identified in <i>P. cyrtonema</i> rhizomes,...

Full description

Saved in:
Bibliographic Details
Main Authors: Shuzhen Wang, Feng He, Ruibin Hu, Xuchun Wan, Wei Wu, Lei Zhang, Chi-Tang Ho, Shiming Li
Format: Article
Language:English
Published: MDPI AG 2025-04-01
Series:Molecules
Subjects:
Online Access:https://www.mdpi.com/1420-3049/30/9/1923
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1849312932315267072
author Shuzhen Wang
Feng He
Ruibin Hu
Xuchun Wan
Wei Wu
Lei Zhang
Chi-Tang Ho
Shiming Li
author_facet Shuzhen Wang
Feng He
Ruibin Hu
Xuchun Wan
Wei Wu
Lei Zhang
Chi-Tang Ho
Shiming Li
author_sort Shuzhen Wang
collection DOAJ
description Polygonati Rhizoma, widely used as a traditional functional food and herbal medicine, is well known for its health-promoting activities after the process of “nine cycles of steaming-drying”. Based on UPLC-MS/MS, 1369 secondary metabolites were identified in <i>P. cyrtonema</i> rhizomes, mainly alkaloids, amino acids and derivatives, flavonoids, organic acids, phenolic acids, and saccharides. The <i>P. cyrtonema</i> rhizomes were rich in xylose, arabinose, glucose, sorbose, mannose, galactose, rhamnose, inositol, fucose, sedoheptulose, phosphorylated monosaccharides, sugar acid, and sugar alcohols. Particularly, 23 types of modifications were detected for amino acids, while the most frequent modifications were acetylation, methylation (nono-, di-, and tri-), cyclo-, homo-, and hydroxylation. Based on the metabolic profile, samples from the third cycle (Tre-3) and the sixth cycle (Tre-6) were firstly clustered together due to similar metabolites and then grouped with samples from the ninth cycle (Tre-9). Differentially accumulated metabolites were mainly enriched in “Metabolic pathways”, “Biosynthesis of cofactors”, “Biosynthesis of secondary metabolites”, “Flavonoid biosynthesis”, “Purine metabolism”, “ABC transporters”, “Biosynthesis of amino acids”, and “Nucleotide metabolism” pathways. During repeated steaming–drying processes, 39 metabolites occurred, including alkaloids, amino acids and derivatives, flavonoids, lignans and coumarins, lipids, nucleotides and derivatives, organic acids, phenolic acids, and terpenoids. This research will provide a critical scientific basis for postharvest processing of <i>P. cyrtonema</i> rhizomes.
format Article
id doaj-art-de42b2f9b7924280a890d377412aab2d
institution Kabale University
issn 1420-3049
language English
publishDate 2025-04-01
publisher MDPI AG
record_format Article
series Molecules
spelling doaj-art-de42b2f9b7924280a890d377412aab2d2025-08-20T03:52:56ZengMDPI AGMolecules1420-30492025-04-01309192310.3390/molecules30091923Dynamic Variation of Secondary Metabolites from <i>Polygonatum cyrtonema</i> Hua Rhizomes During Repeated Steaming–Drying ProcessesShuzhen Wang0Feng He1Ruibin Hu2Xuchun Wan3Wei Wu4Lei Zhang5Chi-Tang Ho6Shiming Li7Lishizhen College of Traditional Chinese Medicine, Huanggang Normal University, Huanggang 438000, ChinaLishizhen College of Traditional Chinese Medicine, Huanggang Normal University, Huanggang 438000, ChinaLishizhen College of Traditional Chinese Medicine, Huanggang Normal University, Huanggang 438000, ChinaLishizhen College of Traditional Chinese Medicine, Huanggang Normal University, Huanggang 438000, ChinaLishizhen College of Traditional Chinese Medicine, Huanggang Normal University, Huanggang 438000, ChinaLishizhen College of Traditional Chinese Medicine, Huanggang Normal University, Huanggang 438000, ChinaDepartment of Food Science, Rutgers University, New Brunswick, NJ 08901, USALishizhen College of Traditional Chinese Medicine, Huanggang Normal University, Huanggang 438000, ChinaPolygonati Rhizoma, widely used as a traditional functional food and herbal medicine, is well known for its health-promoting activities after the process of “nine cycles of steaming-drying”. Based on UPLC-MS/MS, 1369 secondary metabolites were identified in <i>P. cyrtonema</i> rhizomes, mainly alkaloids, amino acids and derivatives, flavonoids, organic acids, phenolic acids, and saccharides. The <i>P. cyrtonema</i> rhizomes were rich in xylose, arabinose, glucose, sorbose, mannose, galactose, rhamnose, inositol, fucose, sedoheptulose, phosphorylated monosaccharides, sugar acid, and sugar alcohols. Particularly, 23 types of modifications were detected for amino acids, while the most frequent modifications were acetylation, methylation (nono-, di-, and tri-), cyclo-, homo-, and hydroxylation. Based on the metabolic profile, samples from the third cycle (Tre-3) and the sixth cycle (Tre-6) were firstly clustered together due to similar metabolites and then grouped with samples from the ninth cycle (Tre-9). Differentially accumulated metabolites were mainly enriched in “Metabolic pathways”, “Biosynthesis of cofactors”, “Biosynthesis of secondary metabolites”, “Flavonoid biosynthesis”, “Purine metabolism”, “ABC transporters”, “Biosynthesis of amino acids”, and “Nucleotide metabolism” pathways. During repeated steaming–drying processes, 39 metabolites occurred, including alkaloids, amino acids and derivatives, flavonoids, lignans and coumarins, lipids, nucleotides and derivatives, organic acids, phenolic acids, and terpenoids. This research will provide a critical scientific basis for postharvest processing of <i>P. cyrtonema</i> rhizomes.https://www.mdpi.com/1420-3049/30/9/1923Polygonati Rhizoma<i>Polygonatum cyrtonema</i>homology of medicine and foodhealth-promoting activitiesmetabolomicspostharvest processing
spellingShingle Shuzhen Wang
Feng He
Ruibin Hu
Xuchun Wan
Wei Wu
Lei Zhang
Chi-Tang Ho
Shiming Li
Dynamic Variation of Secondary Metabolites from <i>Polygonatum cyrtonema</i> Hua Rhizomes During Repeated Steaming–Drying Processes
Molecules
Polygonati Rhizoma
<i>Polygonatum cyrtonema</i>
homology of medicine and food
health-promoting activities
metabolomics
postharvest processing
title Dynamic Variation of Secondary Metabolites from <i>Polygonatum cyrtonema</i> Hua Rhizomes During Repeated Steaming–Drying Processes
title_full Dynamic Variation of Secondary Metabolites from <i>Polygonatum cyrtonema</i> Hua Rhizomes During Repeated Steaming–Drying Processes
title_fullStr Dynamic Variation of Secondary Metabolites from <i>Polygonatum cyrtonema</i> Hua Rhizomes During Repeated Steaming–Drying Processes
title_full_unstemmed Dynamic Variation of Secondary Metabolites from <i>Polygonatum cyrtonema</i> Hua Rhizomes During Repeated Steaming–Drying Processes
title_short Dynamic Variation of Secondary Metabolites from <i>Polygonatum cyrtonema</i> Hua Rhizomes During Repeated Steaming–Drying Processes
title_sort dynamic variation of secondary metabolites from i polygonatum cyrtonema i hua rhizomes during repeated steaming drying processes
topic Polygonati Rhizoma
<i>Polygonatum cyrtonema</i>
homology of medicine and food
health-promoting activities
metabolomics
postharvest processing
url https://www.mdpi.com/1420-3049/30/9/1923
work_keys_str_mv AT shuzhenwang dynamicvariationofsecondarymetabolitesfromipolygonatumcyrtonemaihuarhizomesduringrepeatedsteamingdryingprocesses
AT fenghe dynamicvariationofsecondarymetabolitesfromipolygonatumcyrtonemaihuarhizomesduringrepeatedsteamingdryingprocesses
AT ruibinhu dynamicvariationofsecondarymetabolitesfromipolygonatumcyrtonemaihuarhizomesduringrepeatedsteamingdryingprocesses
AT xuchunwan dynamicvariationofsecondarymetabolitesfromipolygonatumcyrtonemaihuarhizomesduringrepeatedsteamingdryingprocesses
AT weiwu dynamicvariationofsecondarymetabolitesfromipolygonatumcyrtonemaihuarhizomesduringrepeatedsteamingdryingprocesses
AT leizhang dynamicvariationofsecondarymetabolitesfromipolygonatumcyrtonemaihuarhizomesduringrepeatedsteamingdryingprocesses
AT chitangho dynamicvariationofsecondarymetabolitesfromipolygonatumcyrtonemaihuarhizomesduringrepeatedsteamingdryingprocesses
AT shimingli dynamicvariationofsecondarymetabolitesfromipolygonatumcyrtonemaihuarhizomesduringrepeatedsteamingdryingprocesses