Comparative Transcriptome Analysis Reveals Effects of Exogenous Hematin on Anthocyanin Biosynthesis during Strawberry Fruit Ripening
Anthocyanin in strawberries has a positive effect on fruit coloration. In this study, the role of exogenous hematin on anthocyanin biosynthesis was investigated. Our result showed that the white stage of strawberries treated with exogenous hematin had higher anthocyanin content, compared to the cont...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | International Journal of Genomics |
| Online Access: | http://dx.doi.org/10.1155/2016/6762731 |
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| author | Yi Li Huayin Li Fengde Wang Jingjuan Li Yihui Zhang Liangju Wang Jianwei Gao |
| author_facet | Yi Li Huayin Li Fengde Wang Jingjuan Li Yihui Zhang Liangju Wang Jianwei Gao |
| author_sort | Yi Li |
| collection | DOAJ |
| description | Anthocyanin in strawberries has a positive effect on fruit coloration. In this study, the role of exogenous hematin on anthocyanin biosynthesis was investigated. Our result showed that the white stage of strawberries treated with exogenous hematin had higher anthocyanin content, compared to the control group. Among all treatments, 5 μM of hematin was the optimal condition to promote color development. In order to explore the molecular mechanism of fruit coloring regulated by hematin, transcriptomes in the hematin- and non-hematin-treated fruit were analyzed. A large number of differentially expressed genes (DEGs) were identified in regulating anthocyanin synthesis, including the DEGs involved in anthocyanin biosynthesis, hormone signaling transduction, phytochrome signaling, starch and sucrose degradation, and transcriptional pathways. These regulatory networks may play an important role in regulating the color process of strawberries treated with hematin. In summary, exogenous hematin could promote fruit coloring by increasing anthocyanin content in the white stage of strawberries. Furthermore, transcriptome analysis suggests that hematin-promoted fruit coloring occurs through multiple related metabolic pathways, which provides valuable information for regulating fruit color via anthocyanin biosynthesis in strawberries. |
| format | Article |
| id | doaj-art-de3d0afcdab74f3c8cd5eac93aee8ff1 |
| institution | OA Journals |
| issn | 2314-436X 2314-4378 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Genomics |
| spelling | doaj-art-de3d0afcdab74f3c8cd5eac93aee8ff12025-08-20T02:21:20ZengWileyInternational Journal of Genomics2314-436X2314-43782016-01-01201610.1155/2016/67627316762731Comparative Transcriptome Analysis Reveals Effects of Exogenous Hematin on Anthocyanin Biosynthesis during Strawberry Fruit RipeningYi Li0Huayin Li1Fengde Wang2Jingjuan Li3Yihui Zhang4Liangju Wang5Jianwei Gao6Institute of Vegetables and Flowers, Shandong Academy of Agricultural Sciences and Shandong Key Laboratory of Greenhouse Vegetable Biology and Shandong Branch of National Vegetable Improvement Center, Jinan 250100, ChinaInstitute of Vegetables and Flowers, Shandong Academy of Agricultural Sciences and Shandong Key Laboratory of Greenhouse Vegetable Biology and Shandong Branch of National Vegetable Improvement Center, Jinan 250100, ChinaInstitute of Vegetables and Flowers, Shandong Academy of Agricultural Sciences and Shandong Key Laboratory of Greenhouse Vegetable Biology and Shandong Branch of National Vegetable Improvement Center, Jinan 250100, ChinaInstitute of Vegetables and Flowers, Shandong Academy of Agricultural Sciences and Shandong Key Laboratory of Greenhouse Vegetable Biology and Shandong Branch of National Vegetable Improvement Center, Jinan 250100, ChinaInstitute of Vegetables and Flowers, Shandong Academy of Agricultural Sciences and Shandong Key Laboratory of Greenhouse Vegetable Biology and Shandong Branch of National Vegetable Improvement Center, Jinan 250100, ChinaCollege of Horticulture, Nanjing Agricultural University, Nanjing 210095, ChinaInstitute of Vegetables and Flowers, Shandong Academy of Agricultural Sciences and Shandong Key Laboratory of Greenhouse Vegetable Biology and Shandong Branch of National Vegetable Improvement Center, Jinan 250100, ChinaAnthocyanin in strawberries has a positive effect on fruit coloration. In this study, the role of exogenous hematin on anthocyanin biosynthesis was investigated. Our result showed that the white stage of strawberries treated with exogenous hematin had higher anthocyanin content, compared to the control group. Among all treatments, 5 μM of hematin was the optimal condition to promote color development. In order to explore the molecular mechanism of fruit coloring regulated by hematin, transcriptomes in the hematin- and non-hematin-treated fruit were analyzed. A large number of differentially expressed genes (DEGs) were identified in regulating anthocyanin synthesis, including the DEGs involved in anthocyanin biosynthesis, hormone signaling transduction, phytochrome signaling, starch and sucrose degradation, and transcriptional pathways. These regulatory networks may play an important role in regulating the color process of strawberries treated with hematin. In summary, exogenous hematin could promote fruit coloring by increasing anthocyanin content in the white stage of strawberries. Furthermore, transcriptome analysis suggests that hematin-promoted fruit coloring occurs through multiple related metabolic pathways, which provides valuable information for regulating fruit color via anthocyanin biosynthesis in strawberries.http://dx.doi.org/10.1155/2016/6762731 |
| spellingShingle | Yi Li Huayin Li Fengde Wang Jingjuan Li Yihui Zhang Liangju Wang Jianwei Gao Comparative Transcriptome Analysis Reveals Effects of Exogenous Hematin on Anthocyanin Biosynthesis during Strawberry Fruit Ripening International Journal of Genomics |
| title | Comparative Transcriptome Analysis Reveals Effects of Exogenous Hematin on Anthocyanin Biosynthesis during Strawberry Fruit Ripening |
| title_full | Comparative Transcriptome Analysis Reveals Effects of Exogenous Hematin on Anthocyanin Biosynthesis during Strawberry Fruit Ripening |
| title_fullStr | Comparative Transcriptome Analysis Reveals Effects of Exogenous Hematin on Anthocyanin Biosynthesis during Strawberry Fruit Ripening |
| title_full_unstemmed | Comparative Transcriptome Analysis Reveals Effects of Exogenous Hematin on Anthocyanin Biosynthesis during Strawberry Fruit Ripening |
| title_short | Comparative Transcriptome Analysis Reveals Effects of Exogenous Hematin on Anthocyanin Biosynthesis during Strawberry Fruit Ripening |
| title_sort | comparative transcriptome analysis reveals effects of exogenous hematin on anthocyanin biosynthesis during strawberry fruit ripening |
| url | http://dx.doi.org/10.1155/2016/6762731 |
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