Development and Characterization of PEGylated Poly D,L-Lactic Acid Nanoparticles for Skin Rejuvenation
Recently, various biocompatible and biodegradable materials have garnered significant attention as cosmetic fillers for skin rejuvenation. Among these, poly ε-caprolactone (PCL), poly L-lactic acid (PLLA), poly D,L-lactic acid (PDLLA), and polydioxanone (PDO) microspheres have been developed and com...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-03-01
|
| Series: | Nanomaterials |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-4991/15/6/470 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850090960928636928 |
|---|---|
| author | Seunghwa Lee Hyoung-Wook Moon Seong-Jin Lee Jin-Cheol Cho |
| author_facet | Seunghwa Lee Hyoung-Wook Moon Seong-Jin Lee Jin-Cheol Cho |
| author_sort | Seunghwa Lee |
| collection | DOAJ |
| description | Recently, various biocompatible and biodegradable materials have garnered significant attention as cosmetic fillers for skin rejuvenation. Among these, poly ε-caprolactone (PCL), poly L-lactic acid (PLLA), poly D,L-lactic acid (PDLLA), and polydioxanone (PDO) microspheres have been developed and commercialized as a dermal filler. However, its irregularly hydrophobic microspheres pose hydration challenges, often causing syringe needle blockages and side effects such as delayed onset nodules and papules after the procedure. In this study, we synthesized a polyethylene glycol-poly D,L-lactic acid (mPEG-PDLLA) copolymer to address the limitations of conventional polymer fillers. Comprehensive characterization of the copolymer was performed using nuclear magnetic resonance spectroscopy, Fourier transform infrared spectroscopy, and differential scanning calorimetry. The mPEG-PDLLA copolymers demonstrated a unimodal size distribution of approximately 121 ± 20 nm in an aqueous solution. The in vitro cytotoxicity and collagen genesis of mPEG-PDLLA copolymers were evaluated using human dermal fibroblast cells. In this study, angiogenesis was observed over time in hairless mice injected with mPEG-PDLLA copolymers, confirming its potential role in enhancing collagen synthesis. To assess the inflammatory response, the expression levels of the genes MMP1 and IL-1β were analyzed. Additionally, gene expression levels such as transforming growth factor-β and collagen types I and III were compared with Rejuran<sup>®</sup> in animal studies. The newly developed collagen-stimulating PEGylated PDLLA may be a safe and effective option for skin rejuvenation. |
| format | Article |
| id | doaj-art-de2c158f93ee43af928338760da2af4f |
| institution | DOAJ |
| issn | 2079-4991 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj-art-de2c158f93ee43af928338760da2af4f2025-08-20T02:42:28ZengMDPI AGNanomaterials2079-49912025-03-0115647010.3390/nano15060470Development and Characterization of PEGylated Poly D,L-Lactic Acid Nanoparticles for Skin RejuvenationSeunghwa Lee0Hyoung-Wook Moon1Seong-Jin Lee2Jin-Cheol Cho3R&D Center, CHA Meditech Co., Ltd., 119 Techno 2-ro (#206, Migun Techno World, Yongsan-Dong), Yuseong-gu, Daejeon 34116, Republic of KoreaR&D Center, CHA Meditech Co., Ltd., 119 Techno 2-ro (#206, Migun Techno World, Yongsan-Dong), Yuseong-gu, Daejeon 34116, Republic of KoreaR&D Center, CHA Meditech Co., Ltd., 119 Techno 2-ro (#206, Migun Techno World, Yongsan-Dong), Yuseong-gu, Daejeon 34116, Republic of KoreaR&D Center, CHA Meditech Co., Ltd., 119 Techno 2-ro (#206, Migun Techno World, Yongsan-Dong), Yuseong-gu, Daejeon 34116, Republic of KoreaRecently, various biocompatible and biodegradable materials have garnered significant attention as cosmetic fillers for skin rejuvenation. Among these, poly ε-caprolactone (PCL), poly L-lactic acid (PLLA), poly D,L-lactic acid (PDLLA), and polydioxanone (PDO) microspheres have been developed and commercialized as a dermal filler. However, its irregularly hydrophobic microspheres pose hydration challenges, often causing syringe needle blockages and side effects such as delayed onset nodules and papules after the procedure. In this study, we synthesized a polyethylene glycol-poly D,L-lactic acid (mPEG-PDLLA) copolymer to address the limitations of conventional polymer fillers. Comprehensive characterization of the copolymer was performed using nuclear magnetic resonance spectroscopy, Fourier transform infrared spectroscopy, and differential scanning calorimetry. The mPEG-PDLLA copolymers demonstrated a unimodal size distribution of approximately 121 ± 20 nm in an aqueous solution. The in vitro cytotoxicity and collagen genesis of mPEG-PDLLA copolymers were evaluated using human dermal fibroblast cells. In this study, angiogenesis was observed over time in hairless mice injected with mPEG-PDLLA copolymers, confirming its potential role in enhancing collagen synthesis. To assess the inflammatory response, the expression levels of the genes MMP1 and IL-1β were analyzed. Additionally, gene expression levels such as transforming growth factor-β and collagen types I and III were compared with Rejuran<sup>®</sup> in animal studies. The newly developed collagen-stimulating PEGylated PDLLA may be a safe and effective option for skin rejuvenation.https://www.mdpi.com/2079-4991/15/6/470PEGylationPDLLAcollagenin vivoskin rejuvenation |
| spellingShingle | Seunghwa Lee Hyoung-Wook Moon Seong-Jin Lee Jin-Cheol Cho Development and Characterization of PEGylated Poly D,L-Lactic Acid Nanoparticles for Skin Rejuvenation Nanomaterials PEGylation PDLLA collagen in vivo skin rejuvenation |
| title | Development and Characterization of PEGylated Poly D,L-Lactic Acid Nanoparticles for Skin Rejuvenation |
| title_full | Development and Characterization of PEGylated Poly D,L-Lactic Acid Nanoparticles for Skin Rejuvenation |
| title_fullStr | Development and Characterization of PEGylated Poly D,L-Lactic Acid Nanoparticles for Skin Rejuvenation |
| title_full_unstemmed | Development and Characterization of PEGylated Poly D,L-Lactic Acid Nanoparticles for Skin Rejuvenation |
| title_short | Development and Characterization of PEGylated Poly D,L-Lactic Acid Nanoparticles for Skin Rejuvenation |
| title_sort | development and characterization of pegylated poly d l lactic acid nanoparticles for skin rejuvenation |
| topic | PEGylation PDLLA collagen in vivo skin rejuvenation |
| url | https://www.mdpi.com/2079-4991/15/6/470 |
| work_keys_str_mv | AT seunghwalee developmentandcharacterizationofpegylatedpolydllacticacidnanoparticlesforskinrejuvenation AT hyoungwookmoon developmentandcharacterizationofpegylatedpolydllacticacidnanoparticlesforskinrejuvenation AT seongjinlee developmentandcharacterizationofpegylatedpolydllacticacidnanoparticlesforskinrejuvenation AT jincheolcho developmentandcharacterizationofpegylatedpolydllacticacidnanoparticlesforskinrejuvenation |