Optimization of fermentation process for tangerine peel mead by response surface methodology

Using tangerine peel and honey as the main raw materials, the tangerine peel mead was prepared by fermentation with Saccharomyces cerevisiae LA-FR. Using sensory evaluation as the main evaluation index, the fermentation process of tangerine peel mead was optimized through single factor tests and res...

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Main Author: GUO Nannan, ZHANG Xinru, ZHANG Jie
Format: Article
Language:English
Published: Editorial Department of China Brewing 2025-06-01
Series:Zhongguo niangzao
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Online Access:https://manu61.magtech.com.cn/zgnz/fileup/0254-5071/PDF/0254-5071-2025-44-6-205.pdf
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author GUO Nannan, ZHANG Xinru, ZHANG Jie
author_facet GUO Nannan, ZHANG Xinru, ZHANG Jie
author_sort GUO Nannan, ZHANG Xinru, ZHANG Jie
collection DOAJ
description Using tangerine peel and honey as the main raw materials, the tangerine peel mead was prepared by fermentation with Saccharomyces cerevisiae LA-FR. Using sensory evaluation as the main evaluation index, the fermentation process of tangerine peel mead was optimized through single factor tests and response surface methodology. And the physicochemical indicators and volatile flavor components of tangerine peel mead were detected and analyzed by conventional detection methods and gas chromatography-tandem mass spectrometry (GC-MS/MS). The results showed that the optimum fermentation process conditions for tangerine peel mead were as follows: tangerine peel addition 1.7%, initial soluble solid content 23%, yeast inoculum 0.11%, initial pH 4.0, and fermentation temperature 28 ℃. The tangerine peel mead brewed under these optimized conditions was amber in color, clear and transparent, with the fragrance of tangerine peel and honey, rich taste, and mellow flavor, and the sensory score was 92.24 points. The alcohol content was 10.60%vol, the total acid content (calculated as tartaric acid) was 4.35 g/L, and the soluble solids content was 9.50%. The total bacterial counts were 51 CFU/ml, no coliform bacteria were detected, and all indicators met the requirements of QB/T 5475-2020 "Mead". A total of 17 major volatile flavor components were detected in tangerine peel mead, including 6 alcohols, 8 esters, and 3 alkenes. Among them, the ethanol (28.36%), D-limonene (18.8%), 2-methylbutanol (16.22%) and phenylethanol (12.07%) had relatively high relative contents.
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spelling doaj-art-de27cd96040048cfbaa034b6c49f788d2025-08-20T02:59:58ZengEditorial Department of China BrewingZhongguo niangzao0254-50712025-06-0144620521110.11882/j.issn.0254-5071.2025.06.029Optimization of fermentation process for tangerine peel mead by response surface methodologyGUO Nannan, ZHANG Xinru, ZHANG Jie0Zhengzhou Rapid Detection Technology of Food Safety Laboratory, Zhengzhou University of Science and Technology, Zhengzhou 450064, ChinaUsing tangerine peel and honey as the main raw materials, the tangerine peel mead was prepared by fermentation with Saccharomyces cerevisiae LA-FR. Using sensory evaluation as the main evaluation index, the fermentation process of tangerine peel mead was optimized through single factor tests and response surface methodology. And the physicochemical indicators and volatile flavor components of tangerine peel mead were detected and analyzed by conventional detection methods and gas chromatography-tandem mass spectrometry (GC-MS/MS). The results showed that the optimum fermentation process conditions for tangerine peel mead were as follows: tangerine peel addition 1.7%, initial soluble solid content 23%, yeast inoculum 0.11%, initial pH 4.0, and fermentation temperature 28 ℃. The tangerine peel mead brewed under these optimized conditions was amber in color, clear and transparent, with the fragrance of tangerine peel and honey, rich taste, and mellow flavor, and the sensory score was 92.24 points. The alcohol content was 10.60%vol, the total acid content (calculated as tartaric acid) was 4.35 g/L, and the soluble solids content was 9.50%. The total bacterial counts were 51 CFU/ml, no coliform bacteria were detected, and all indicators met the requirements of QB/T 5475-2020 "Mead". A total of 17 major volatile flavor components were detected in tangerine peel mead, including 6 alcohols, 8 esters, and 3 alkenes. Among them, the ethanol (28.36%), D-limonene (18.8%), 2-methylbutanol (16.22%) and phenylethanol (12.07%) had relatively high relative contents.https://manu61.magtech.com.cn/zgnz/fileup/0254-5071/PDF/0254-5071-2025-44-6-205.pdftangerine peel mead|response surface methodology|fermentation process|sensory score|physicochemical indicator|volatile flavor component
spellingShingle GUO Nannan, ZHANG Xinru, ZHANG Jie
Optimization of fermentation process for tangerine peel mead by response surface methodology
Zhongguo niangzao
tangerine peel mead|response surface methodology|fermentation process|sensory score|physicochemical indicator|volatile flavor component
title Optimization of fermentation process for tangerine peel mead by response surface methodology
title_full Optimization of fermentation process for tangerine peel mead by response surface methodology
title_fullStr Optimization of fermentation process for tangerine peel mead by response surface methodology
title_full_unstemmed Optimization of fermentation process for tangerine peel mead by response surface methodology
title_short Optimization of fermentation process for tangerine peel mead by response surface methodology
title_sort optimization of fermentation process for tangerine peel mead by response surface methodology
topic tangerine peel mead|response surface methodology|fermentation process|sensory score|physicochemical indicator|volatile flavor component
url https://manu61.magtech.com.cn/zgnz/fileup/0254-5071/PDF/0254-5071-2025-44-6-205.pdf
work_keys_str_mv AT guonannanzhangxinruzhangjie optimizationoffermentationprocessfortangerinepeelmeadbyresponsesurfacemethodology