Biomimetic gender-specific human skin model based on gonads/epidermis-on-a-chip

The development of bionic skin is crucial for progressing our understanding of skin physiology and pathologies, while the precise replication of human skin features in skin models remains a challenge. In this study, we present a biomimetic, gender-specific skin model utilizing a gonads/epidermis-on-...

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Main Authors: Xiangyi Wu, Yuanyuan Zhang, Ye Xu, Xinyue Cao, Yuanjin Zhao
Format: Article
Language:English
Published: KeAi Communications Co., Ltd. 2025-11-01
Series:Bioactive Materials
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Online Access:http://www.sciencedirect.com/science/article/pii/S2452199X2500307X
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author Xiangyi Wu
Yuanyuan Zhang
Ye Xu
Xinyue Cao
Yuanjin Zhao
author_facet Xiangyi Wu
Yuanyuan Zhang
Ye Xu
Xinyue Cao
Yuanjin Zhao
author_sort Xiangyi Wu
collection DOAJ
description The development of bionic skin is crucial for progressing our understanding of skin physiology and pathologies, while the precise replication of human skin features in skin models remains a challenge. In this study, we present a biomimetic, gender-specific skin model utilizing a gonads/epidermis-on-a-chip platform. This innovative approach seamlessly integrates ex vivo cultured human epidermal tissue and gonad cell aggregates into a microfluidic system, facilitating the exploration of the regulatory effects of sex hormones on the epidermis. Based on this platform, we have found that estradiol can enhance proliferation and inhibit apoptosis of keratinocytes. Conversely, testosterone notably induces keratinocyte differentiation, leading to epidermal hyperkeratosis. These observations align with the well-documented phenomenon that males possess thicker and more durable skin, while females’ skin exhibits superior repair capabilities. Thus, we believe that our biomimetic gender-specific skin model can offer a promising avenue for obtaining higher-certainty evidence of human skin-related conditions and find important applications in the areas of cosmetics and biomedicine.
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publisher KeAi Communications Co., Ltd.
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spelling doaj-art-de220621fd104f67b97759101e786c342025-08-20T02:39:16ZengKeAi Communications Co., Ltd.Bioactive Materials2452-199X2025-11-015324025210.1016/j.bioactmat.2025.07.014Biomimetic gender-specific human skin model based on gonads/epidermis-on-a-chipXiangyi Wu0Yuanyuan Zhang1Ye Xu2Xinyue Cao3Yuanjin Zhao4Department of Burn and Plastic Surgery, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, 210008, ChinaDepartment of Dermatology and Venereology, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, 210008, ChinaDepartment of Burn and Plastic Surgery, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, 210008, ChinaSchool of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, ChinaDepartment of Burn and Plastic Surgery, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, 210008, China; School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, China; Corresponding author. Department of Burn and Plastic Surgery, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, 210008, China.The development of bionic skin is crucial for progressing our understanding of skin physiology and pathologies, while the precise replication of human skin features in skin models remains a challenge. In this study, we present a biomimetic, gender-specific skin model utilizing a gonads/epidermis-on-a-chip platform. This innovative approach seamlessly integrates ex vivo cultured human epidermal tissue and gonad cell aggregates into a microfluidic system, facilitating the exploration of the regulatory effects of sex hormones on the epidermis. Based on this platform, we have found that estradiol can enhance proliferation and inhibit apoptosis of keratinocytes. Conversely, testosterone notably induces keratinocyte differentiation, leading to epidermal hyperkeratosis. These observations align with the well-documented phenomenon that males possess thicker and more durable skin, while females’ skin exhibits superior repair capabilities. Thus, we believe that our biomimetic gender-specific skin model can offer a promising avenue for obtaining higher-certainty evidence of human skin-related conditions and find important applications in the areas of cosmetics and biomedicine.http://www.sciencedirect.com/science/article/pii/S2452199X2500307XOrgan-on-a-chipSkin-on-a-chipGonad-on-a-chipMicrofluidicsSkin modelBiomimetic
spellingShingle Xiangyi Wu
Yuanyuan Zhang
Ye Xu
Xinyue Cao
Yuanjin Zhao
Biomimetic gender-specific human skin model based on gonads/epidermis-on-a-chip
Bioactive Materials
Organ-on-a-chip
Skin-on-a-chip
Gonad-on-a-chip
Microfluidics
Skin model
Biomimetic
title Biomimetic gender-specific human skin model based on gonads/epidermis-on-a-chip
title_full Biomimetic gender-specific human skin model based on gonads/epidermis-on-a-chip
title_fullStr Biomimetic gender-specific human skin model based on gonads/epidermis-on-a-chip
title_full_unstemmed Biomimetic gender-specific human skin model based on gonads/epidermis-on-a-chip
title_short Biomimetic gender-specific human skin model based on gonads/epidermis-on-a-chip
title_sort biomimetic gender specific human skin model based on gonads epidermis on a chip
topic Organ-on-a-chip
Skin-on-a-chip
Gonad-on-a-chip
Microfluidics
Skin model
Biomimetic
url http://www.sciencedirect.com/science/article/pii/S2452199X2500307X
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AT yexu biomimeticgenderspecifichumanskinmodelbasedongonadsepidermisonachip
AT xinyuecao biomimeticgenderspecifichumanskinmodelbasedongonadsepidermisonachip
AT yuanjinzhao biomimeticgenderspecifichumanskinmodelbasedongonadsepidermisonachip