Screening of winter triticale genotypes for resistance to salinity in the shoot apical meristem culture
Purpose. To conduct in vitro screening of different genotypes of winter triticale for resistance to salinity in the shoot apical meristem culture. Methods. Plant tissue culture in vitro, in vitro breeding, statistical analysis. Results. It was found that the increase of sodium chloride concentra...
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| Format: | Article |
| Language: | English |
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Ukrainian Institute for Plant Variety Examination
2017-09-01
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| Series: | Plant Varieties Studying and Protection |
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| Online Access: | http://journal.sops.gov.ua/article/view/110710 |
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| author | С. В. Пикало О. В. Дубровна |
| author_facet | С. В. Пикало О. В. Дубровна |
| author_sort | С. В. Пикало |
| collection | DOAJ |
| description | Purpose. To conduct in vitro screening of different genotypes of winter triticale for resistance to salinity in the shoot apical meristem culture.
Methods. Plant tissue culture in vitro, in vitro breeding, statistical analysis.
Results. It was found that the increase of sodium chloride concentration from 0.6 to 1.5% resulted in inhibition of the callus culture growth in all genotypes that was indicative of the toxic effect of the stress factor. It turns out that 1.2% sodium chloride concentration allowed to differentiate triticale genotypes for salt tolerance. The line ‘38/1296’ appeared to be the most resistant to salinity stress because under breeding conditions calli of this genotype were characterized by higher morphogenetic potential, had the highest crude mass increase, and plants-regenerants were obtained only from explants of this line after cultivation on the medium containing 1.5% sodium chloride. The ‘ADM 11’ variety was the most sensitive to saline stress as mass necrosis and lack of regenerative ability in its calli were observed under breeding conditions. In the studied forms, genotypic dependence of morphogenesis processes in vitro culture was registered. From the induced calli, plants-regenerants were obtained, and their completion of growing, root development and transfer to in vivo conditions were optimized.
Conclusions. Genotypic response to salinity stress in the culture of shoot apical meristems of winter triticale was expressed by various crude mass increase and different morphogenetic potential on exposure to a stress factor. The line ‘38/1296’ can be used as a valuable material for further breeding of winter triticale. The culture of shoot apical meristems is recommended to apply as a test system for screening of triticale genotypes for resistance to salinity stress |
| format | Article |
| id | doaj-art-de20b460d52141228e50bbbf616070bf |
| institution | OA Journals |
| issn | 2518-1017 2518-7457 |
| language | English |
| publishDate | 2017-09-01 |
| publisher | Ukrainian Institute for Plant Variety Examination |
| record_format | Article |
| series | Plant Varieties Studying and Protection |
| spelling | doaj-art-de20b460d52141228e50bbbf616070bf2025-08-20T01:48:07ZengUkrainian Institute for Plant Variety ExaminationPlant Varieties Studying and Protection2518-10172518-74572017-09-0113327728410.21498/2518-1017.13.3.2017.110710110710Screening of winter triticale genotypes for resistance to salinity in the shoot apical meristem cultureС. В. Пикало0О. В. Дубровна1The V. M. Remeslo Myronivka Institute of Wheat, NAAS of UkraineInstitute of Plant Physiology and Genetics, NAS of UkrainePurpose. To conduct in vitro screening of different genotypes of winter triticale for resistance to salinity in the shoot apical meristem culture. Methods. Plant tissue culture in vitro, in vitro breeding, statistical analysis. Results. It was found that the increase of sodium chloride concentration from 0.6 to 1.5% resulted in inhibition of the callus culture growth in all genotypes that was indicative of the toxic effect of the stress factor. It turns out that 1.2% sodium chloride concentration allowed to differentiate triticale genotypes for salt tolerance. The line ‘38/1296’ appeared to be the most resistant to salinity stress because under breeding conditions calli of this genotype were characterized by higher morphogenetic potential, had the highest crude mass increase, and plants-regenerants were obtained only from explants of this line after cultivation on the medium containing 1.5% sodium chloride. The ‘ADM 11’ variety was the most sensitive to saline stress as mass necrosis and lack of regenerative ability in its calli were observed under breeding conditions. In the studied forms, genotypic dependence of morphogenesis processes in vitro culture was registered. From the induced calli, plants-regenerants were obtained, and their completion of growing, root development and transfer to in vivo conditions were optimized. Conclusions. Genotypic response to salinity stress in the culture of shoot apical meristems of winter triticale was expressed by various crude mass increase and different morphogenetic potential on exposure to a stress factor. The line ‘38/1296’ can be used as a valuable material for further breeding of winter triticale. The culture of shoot apical meristems is recommended to apply as a test system for screening of triticale genotypes for resistance to salinity stresshttp://journal.sops.gov.ua/article/view/110710winter triticalesalinity stressresistancecallus |
| spellingShingle | С. В. Пикало О. В. Дубровна Screening of winter triticale genotypes for resistance to salinity in the shoot apical meristem culture Plant Varieties Studying and Protection winter triticale salinity stress resistance callus |
| title | Screening of winter triticale genotypes for resistance to salinity in the shoot apical meristem culture |
| title_full | Screening of winter triticale genotypes for resistance to salinity in the shoot apical meristem culture |
| title_fullStr | Screening of winter triticale genotypes for resistance to salinity in the shoot apical meristem culture |
| title_full_unstemmed | Screening of winter triticale genotypes for resistance to salinity in the shoot apical meristem culture |
| title_short | Screening of winter triticale genotypes for resistance to salinity in the shoot apical meristem culture |
| title_sort | screening of winter triticale genotypes for resistance to salinity in the shoot apical meristem culture |
| topic | winter triticale salinity stress resistance callus |
| url | http://journal.sops.gov.ua/article/view/110710 |
| work_keys_str_mv | AT svpikalo screeningofwintertriticalegenotypesforresistancetosalinityintheshootapicalmeristemculture AT ovdubrovna screeningofwintertriticalegenotypesforresistancetosalinityintheshootapicalmeristemculture |