Investigation of the Pathogenesis of Lumpy Skin Disease Virus in Indigenous Cattle in Kazakhstan
This study investigates the virulence properties and pathogenetic characteristics of the Kazakhstani strain of LSDV (LSDV KZ-Kostanay-2018) in indigenous cattle under controlled conditions. Twelve non-breed cattle were inoculated intradermally and monitored for clinical, pathological, and immunologi...
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MDPI AG
2025-06-01
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| Online Access: | https://www.mdpi.com/2076-0817/14/6/577 |
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| author | Lespek Kutumbetov Ainur Ragatova Moldir Azanbekova Balzhan Myrzakhmetova Nurbek Aldayarov Kuandyk Zhugunissov Yergali Abduraimov Raikhan Nissanova Asylay Sarzhigitova Nazerke Kemalova Arman Issimov |
| author_facet | Lespek Kutumbetov Ainur Ragatova Moldir Azanbekova Balzhan Myrzakhmetova Nurbek Aldayarov Kuandyk Zhugunissov Yergali Abduraimov Raikhan Nissanova Asylay Sarzhigitova Nazerke Kemalova Arman Issimov |
| author_sort | Lespek Kutumbetov |
| collection | DOAJ |
| description | This study investigates the virulence properties and pathogenetic characteristics of the Kazakhstani strain of LSDV (LSDV KZ-Kostanay-2018) in indigenous cattle under controlled conditions. Twelve non-breed cattle were inoculated intradermally and monitored for clinical, pathological, and immunological responses. Clinical signs, including fever, skin nodules, and lymphadenopathy, emerged as early as day 5 post-infection (pi), with peak severity observed between days 11 and 14. Rapid seroconversion was observed, with 100% of animals showing virus-neutralizing antibodies by day 13. Pathological findings revealed extensive necrosis, thrombosis, and edema, with pronounced damage in the spleen, lungs, and lymph nodes. Histological analyses identified widespread destructive changes in the dermis and systemic tissues, consistent with highly aggressive disease progression. Viral genome and replication were confirmed in blood, skin nodules, and lymph nodes, with peak viral loads between days 11 and 14 pi. These results align with findings in Russian cattle infected with the Saratov/2017 strain but demonstrate more rapid symptom onset and severe pathology, suggesting strain-specific virulence. These findings contribute to a deeper understanding of LSDV pathogenesis and underscore the importance of regional adaptations in disease management. |
| format | Article |
| id | doaj-art-de122cb98d9f41a782dce4eeaa70d42a |
| institution | OA Journals |
| issn | 2076-0817 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj-art-de122cb98d9f41a782dce4eeaa70d42a2025-08-20T02:21:49ZengMDPI AGPathogens2076-08172025-06-0114657710.3390/pathogens14060577Investigation of the Pathogenesis of Lumpy Skin Disease Virus in Indigenous Cattle in KazakhstanLespek Kutumbetov0Ainur Ragatova1Moldir Azanbekova2Balzhan Myrzakhmetova3Nurbek Aldayarov4Kuandyk Zhugunissov5Yergali Abduraimov6Raikhan Nissanova7Asylay Sarzhigitova8Nazerke Kemalova9Arman Issimov10Research Institute for Biological Safety Problems, Gvardeiskiy 080423, KazakhstanDepartment of Veterinary Medicine, A. Baitursynov Kostanay Regional University, Kostanay 110000, KazakhstanResearch Institute for Biological Safety Problems, Gvardeiskiy 080423, KazakhstanResearch Institute for Biological Safety Problems, Gvardeiskiy 080423, KazakhstanDepartment of Biology, Kyrgyz-Turkish Manas University, Bishkek 720042, KyrgyzstanResearch Institute for Biological Safety Problems, Gvardeiskiy 080423, KazakhstanQazBioPharm National Holding, Astana 010000, KazakhstanDepartment of Virology, Kazakh Scientific Research Veterinary Institute, Almaty 050016, KazakhstanDepartment of Biology, K. Zhubanov Aktobe Regional University, Aktobe 030000, KazakhstanDepartment of Biology, K. Zhubanov Aktobe Regional University, Aktobe 030000, KazakhstanDepartment of Biology, K. Zhubanov Aktobe Regional University, Aktobe 030000, KazakhstanThis study investigates the virulence properties and pathogenetic characteristics of the Kazakhstani strain of LSDV (LSDV KZ-Kostanay-2018) in indigenous cattle under controlled conditions. Twelve non-breed cattle were inoculated intradermally and monitored for clinical, pathological, and immunological responses. Clinical signs, including fever, skin nodules, and lymphadenopathy, emerged as early as day 5 post-infection (pi), with peak severity observed between days 11 and 14. Rapid seroconversion was observed, with 100% of animals showing virus-neutralizing antibodies by day 13. Pathological findings revealed extensive necrosis, thrombosis, and edema, with pronounced damage in the spleen, lungs, and lymph nodes. Histological analyses identified widespread destructive changes in the dermis and systemic tissues, consistent with highly aggressive disease progression. Viral genome and replication were confirmed in blood, skin nodules, and lymph nodes, with peak viral loads between days 11 and 14 pi. These results align with findings in Russian cattle infected with the Saratov/2017 strain but demonstrate more rapid symptom onset and severe pathology, suggesting strain-specific virulence. These findings contribute to a deeper understanding of LSDV pathogenesis and underscore the importance of regional adaptations in disease management.https://www.mdpi.com/2076-0817/14/6/577lumpy skin diseaseLSDVexperimental inoculationKazakhstan |
| spellingShingle | Lespek Kutumbetov Ainur Ragatova Moldir Azanbekova Balzhan Myrzakhmetova Nurbek Aldayarov Kuandyk Zhugunissov Yergali Abduraimov Raikhan Nissanova Asylay Sarzhigitova Nazerke Kemalova Arman Issimov Investigation of the Pathogenesis of Lumpy Skin Disease Virus in Indigenous Cattle in Kazakhstan Pathogens lumpy skin disease LSDV experimental inoculation Kazakhstan |
| title | Investigation of the Pathogenesis of Lumpy Skin Disease Virus in Indigenous Cattle in Kazakhstan |
| title_full | Investigation of the Pathogenesis of Lumpy Skin Disease Virus in Indigenous Cattle in Kazakhstan |
| title_fullStr | Investigation of the Pathogenesis of Lumpy Skin Disease Virus in Indigenous Cattle in Kazakhstan |
| title_full_unstemmed | Investigation of the Pathogenesis of Lumpy Skin Disease Virus in Indigenous Cattle in Kazakhstan |
| title_short | Investigation of the Pathogenesis of Lumpy Skin Disease Virus in Indigenous Cattle in Kazakhstan |
| title_sort | investigation of the pathogenesis of lumpy skin disease virus in indigenous cattle in kazakhstan |
| topic | lumpy skin disease LSDV experimental inoculation Kazakhstan |
| url | https://www.mdpi.com/2076-0817/14/6/577 |
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