A three-sided story: a biosystematic revision of genus Datura reveals novel tropane alkaloids for the first-time in certain species
IntroductionDatura, long considered an medicinal plant, represents a prospective source for discovering novel drugs for modern medicine. The Egyptian flora encompasses six Datura genotypes, including D. innoxia, D. ferox, D. metel and three forms of D. stramonium (stramonium, tatula and inermis). Ho...
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Frontiers Media S.A.
2025-05-01
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2025.1555237/full |
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| author | Abeer Al-Andal Mohamed Ewas Mohamed Ewas Abd El Raheim M. Donia Asmaa M. Radwan Mohamed N. S. Suliman Elsayed Nishawy Ahmed El-Shabasy Eman Khames |
| author_facet | Abeer Al-Andal Mohamed Ewas Mohamed Ewas Abd El Raheim M. Donia Asmaa M. Radwan Mohamed N. S. Suliman Elsayed Nishawy Ahmed El-Shabasy Eman Khames |
| author_sort | Abeer Al-Andal |
| collection | DOAJ |
| description | IntroductionDatura, long considered an medicinal plant, represents a prospective source for discovering novel drugs for modern medicine. The Egyptian flora encompasses six Datura genotypes, including D. innoxia, D. ferox, D. metel and three forms of D. stramonium (stramonium, tatula and inermis). However, the taxonomic status of Datura genus remains controversial.MethodsOur study aims to clarify the phylogenetic relationships among Egyptian Datura via contemporary molecular taxonomy techniques, including ISSR, SCoT, CDDP, as well as cytogenetics assessment, and chemical taxonomy, using total- and tropane-alkaloid and UV spectroscopic pattern.ResultsBased on our results, the three forms of D. stramonium (stramonium, tatula and inermis) are closely related taxa, although there were some significant differences, suggesting the need to upgrade inermis to level of subspecies (Datura stramonium subsp. Inermis). The GC-MS results identified 31 tropane alkaloids. Out of which, seven were described in a qualitative manner in genus Datura, which enhances these genotypes’ medical and economic value. Expression level of the genes PMT, TR1, TR2, H6H, HDH and AT4 influenced the tropane alkaloids accumulation within the genotypes examined.DiscussionTo date, this is the first study to identify the evolutionary relationship of the genus Datura combining molecular and chemical taxonomy, and to quantify the TAs and the genes involved in their biosynthesis among Datura genotypes.ConclusionThis study is significant since drug development strategies and enhanced therapeutic usage of Datura heavily depend on comprehensive knowledge of the species and subspecies’ molecular and phytochemical variability. |
| format | Article |
| id | doaj-art-de0b2c4313c54fd993014ad6ac9fffc8 |
| institution | OA Journals |
| issn | 1664-462X |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj-art-de0b2c4313c54fd993014ad6ac9fffc82025-08-20T02:13:28ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2025-05-011610.3389/fpls.2025.15552371555237A three-sided story: a biosystematic revision of genus Datura reveals novel tropane alkaloids for the first-time in certain speciesAbeer Al-Andal0Mohamed Ewas1Mohamed Ewas2Abd El Raheim M. Donia3Asmaa M. Radwan4Mohamed N. S. Suliman5Elsayed Nishawy6Ahmed El-Shabasy7Eman Khames8Department of Biology, College of Science, King Khalid University, Abha, Saudi ArabiaLaboratory of Genomics and Genome Editing, Genetic and Cytology Unit, Plant Genetic Resources Department, Desert Research Center, Cairo, EgyptDepartment of Biochemistry, Faculty of Pharmacy, Tanta University, Tanta, Gharbia, EgyptNatural Products Unit, Medicinal and Aromatic Plants Department, Desert Research Center, Cairo, EgyptBotany and Microbiology Department, Faculty of Science, Girls Branch, Al-Azhar University, Cairo, EgyptLaboratory of Genomics and Genome Editing, Genetic and Cytology Unit, Plant Genetic Resources Department, Desert Research Center, Cairo, EgyptLaboratory of Genomics and Genome Editing, Genetic and Cytology Unit, Plant Genetic Resources Department, Desert Research Center, Cairo, EgyptDepartment of Biology, College of Science, Jazan University, Jazan, Saudi ArabiaDepartment of Biochemistry, Faculty of Pharmacy, Tanta University, Tanta, Gharbia, EgyptIntroductionDatura, long considered an medicinal plant, represents a prospective source for discovering novel drugs for modern medicine. The Egyptian flora encompasses six Datura genotypes, including D. innoxia, D. ferox, D. metel and three forms of D. stramonium (stramonium, tatula and inermis). However, the taxonomic status of Datura genus remains controversial.MethodsOur study aims to clarify the phylogenetic relationships among Egyptian Datura via contemporary molecular taxonomy techniques, including ISSR, SCoT, CDDP, as well as cytogenetics assessment, and chemical taxonomy, using total- and tropane-alkaloid and UV spectroscopic pattern.ResultsBased on our results, the three forms of D. stramonium (stramonium, tatula and inermis) are closely related taxa, although there were some significant differences, suggesting the need to upgrade inermis to level of subspecies (Datura stramonium subsp. Inermis). The GC-MS results identified 31 tropane alkaloids. Out of which, seven were described in a qualitative manner in genus Datura, which enhances these genotypes’ medical and economic value. Expression level of the genes PMT, TR1, TR2, H6H, HDH and AT4 influenced the tropane alkaloids accumulation within the genotypes examined.DiscussionTo date, this is the first study to identify the evolutionary relationship of the genus Datura combining molecular and chemical taxonomy, and to quantify the TAs and the genes involved in their biosynthesis among Datura genotypes.ConclusionThis study is significant since drug development strategies and enhanced therapeutic usage of Datura heavily depend on comprehensive knowledge of the species and subspecies’ molecular and phytochemical variability.https://www.frontiersin.org/articles/10.3389/fpls.2025.1555237/fullDaturabiosystematic revisioncytogeneticsmolecular markers (CDDPSCoTISSR) |
| spellingShingle | Abeer Al-Andal Mohamed Ewas Mohamed Ewas Abd El Raheim M. Donia Asmaa M. Radwan Mohamed N. S. Suliman Elsayed Nishawy Ahmed El-Shabasy Eman Khames A three-sided story: a biosystematic revision of genus Datura reveals novel tropane alkaloids for the first-time in certain species Frontiers in Plant Science Datura biosystematic revision cytogenetics molecular markers (CDDP SCoT ISSR) |
| title | A three-sided story: a biosystematic revision of genus Datura reveals novel tropane alkaloids for the first-time in certain species |
| title_full | A three-sided story: a biosystematic revision of genus Datura reveals novel tropane alkaloids for the first-time in certain species |
| title_fullStr | A three-sided story: a biosystematic revision of genus Datura reveals novel tropane alkaloids for the first-time in certain species |
| title_full_unstemmed | A three-sided story: a biosystematic revision of genus Datura reveals novel tropane alkaloids for the first-time in certain species |
| title_short | A three-sided story: a biosystematic revision of genus Datura reveals novel tropane alkaloids for the first-time in certain species |
| title_sort | three sided story a biosystematic revision of genus datura reveals novel tropane alkaloids for the first time in certain species |
| topic | Datura biosystematic revision cytogenetics molecular markers (CDDP SCoT ISSR) |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2025.1555237/full |
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