Prognostic Role of Invasion-Related Extracellular Matrix Molecules in Diffusely Infiltrating Grade 2 and 3 Astrocytomas
Background: Astrocytoma, an IDH-mutant is a common primary brain tumor. Total surgical resection is not feasible due to peritumoral infiltration mediated by extracellular matrix (ECM) molecules. Methods: This study aimed at determining the expression pattern of ECM molecules in different prognostic...
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MDPI AG
2024-11-01
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| author | László Szivos József Virga Zoltán Mészár Melinda Rostás Andrea Bakó Gábor Zahuczki Tibor Hortobágyi Álmos Klekner |
| author_facet | László Szivos József Virga Zoltán Mészár Melinda Rostás Andrea Bakó Gábor Zahuczki Tibor Hortobágyi Álmos Klekner |
| author_sort | László Szivos |
| collection | DOAJ |
| description | Background: Astrocytoma, an IDH-mutant is a common primary brain tumor. Total surgical resection is not feasible due to peritumoral infiltration mediated by extracellular matrix (ECM) molecules. Methods: This study aimed at determining the expression pattern of ECM molecules in different prognostic groups of WHO grade 2 and grade 3 patients and identifying the effect of onco-radiotherapy on tumor cell invasion of grade 3 patients. Gene and protein expression of ECM molecules was determined by qRT-PCR and immunohistochemistry, respectively. Results: In the different prognostic groups of grade 2 tumors HMMR, IDH-1, MKI-67, PDGF-A and versican, in grade 3 tumors integrin α-3, and in both groups integrin α-3 and IDH-1 mRNA expression was significantly different. Regarding protein expression, only integrin αV expression changed significantly in the prognostic groups of grade 2 tumors. Conclusions: Based on the invasion spectrum determined by this joint gene and protein expression analysis, there was a sensitivity of 87.5% and a negative predictive value of 88.9% regarding the different prognostic groups of grade 2 astrocytoma. For grade 3 tumors, the applied standard oncotherapeutic modalities apparently lacked significant anti-invasive effects. |
| format | Article |
| id | doaj-art-de09ba9be3e44e0ca61801bb202cb3f2 |
| institution | OA Journals |
| issn | 2076-3425 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
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| series | Brain Sciences |
| spelling | doaj-art-de09ba9be3e44e0ca61801bb202cb3f22025-08-20T02:08:02ZengMDPI AGBrain Sciences2076-34252024-11-011411115710.3390/brainsci14111157Prognostic Role of Invasion-Related Extracellular Matrix Molecules in Diffusely Infiltrating Grade 2 and 3 AstrocytomasLászló Szivos0József Virga1Zoltán Mészár2Melinda Rostás3Andrea Bakó4Gábor Zahuczki5Tibor Hortobágyi6Álmos Klekner7Department of Neurosurgery, University of Debrecen, H-4032 Debrecen, HungaryDepartment of Neurosurgery, University of Debrecen, H-4032 Debrecen, HungaryDepartment of Anatomy, Histology and Embryology, University of Debrecen, H-4032 Debrecen, HungaryDepartment of Biochemistry and Molecular Biology, University of Debrecen, H-4032 Debrecen, HungaryDepartment of Oncology, University of Debrecen, H-4032 Debrecen, HungaryUD-GenoMed Medical Genomic Technologies Ltd., H-4032 Debrecen, HungaryDepartment of Neurology, University of Debrecen, H-4032 Debrecen, HungaryDepartment of Neurosurgery, University of Debrecen, H-4032 Debrecen, HungaryBackground: Astrocytoma, an IDH-mutant is a common primary brain tumor. Total surgical resection is not feasible due to peritumoral infiltration mediated by extracellular matrix (ECM) molecules. Methods: This study aimed at determining the expression pattern of ECM molecules in different prognostic groups of WHO grade 2 and grade 3 patients and identifying the effect of onco-radiotherapy on tumor cell invasion of grade 3 patients. Gene and protein expression of ECM molecules was determined by qRT-PCR and immunohistochemistry, respectively. Results: In the different prognostic groups of grade 2 tumors HMMR, IDH-1, MKI-67, PDGF-A and versican, in grade 3 tumors integrin α-3, and in both groups integrin α-3 and IDH-1 mRNA expression was significantly different. Regarding protein expression, only integrin αV expression changed significantly in the prognostic groups of grade 2 tumors. Conclusions: Based on the invasion spectrum determined by this joint gene and protein expression analysis, there was a sensitivity of 87.5% and a negative predictive value of 88.9% regarding the different prognostic groups of grade 2 astrocytoma. For grade 3 tumors, the applied standard oncotherapeutic modalities apparently lacked significant anti-invasive effects.https://www.mdpi.com/2076-3425/14/11/1157peritumoral infiltrationastrocytomainvasion spectrumextracellular matrixprognostic factor |
| spellingShingle | László Szivos József Virga Zoltán Mészár Melinda Rostás Andrea Bakó Gábor Zahuczki Tibor Hortobágyi Álmos Klekner Prognostic Role of Invasion-Related Extracellular Matrix Molecules in Diffusely Infiltrating Grade 2 and 3 Astrocytomas Brain Sciences peritumoral infiltration astrocytoma invasion spectrum extracellular matrix prognostic factor |
| title | Prognostic Role of Invasion-Related Extracellular Matrix Molecules in Diffusely Infiltrating Grade 2 and 3 Astrocytomas |
| title_full | Prognostic Role of Invasion-Related Extracellular Matrix Molecules in Diffusely Infiltrating Grade 2 and 3 Astrocytomas |
| title_fullStr | Prognostic Role of Invasion-Related Extracellular Matrix Molecules in Diffusely Infiltrating Grade 2 and 3 Astrocytomas |
| title_full_unstemmed | Prognostic Role of Invasion-Related Extracellular Matrix Molecules in Diffusely Infiltrating Grade 2 and 3 Astrocytomas |
| title_short | Prognostic Role of Invasion-Related Extracellular Matrix Molecules in Diffusely Infiltrating Grade 2 and 3 Astrocytomas |
| title_sort | prognostic role of invasion related extracellular matrix molecules in diffusely infiltrating grade 2 and 3 astrocytomas |
| topic | peritumoral infiltration astrocytoma invasion spectrum extracellular matrix prognostic factor |
| url | https://www.mdpi.com/2076-3425/14/11/1157 |
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