Automation of protein crystallization scaleup via Opentrons-2 liquid handling
In this study we present an approach for optimizing protein crystallization trials at the multi-microliter scale utilizing the Opentrons-2 liquid handling robot. Our research demonstrates the robot's capability to automate 24-well sitting drop protein crystallization trials. Using Python script...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-06-01
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| Series: | SLAS Technology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2472630325000263 |
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| author | Jacob B. DeRoo Alec A. Jones Caroline K. Slaughter Tim W. Ahr Sam M. Stroup Grace B. Thompson Christopher D. Snow |
| author_facet | Jacob B. DeRoo Alec A. Jones Caroline K. Slaughter Tim W. Ahr Sam M. Stroup Grace B. Thompson Christopher D. Snow |
| author_sort | Jacob B. DeRoo |
| collection | DOAJ |
| description | In this study we present an approach for optimizing protein crystallization trials at the multi-microliter scale utilizing the Opentrons-2 liquid handling robot. Our research demonstrates the robot's capability to automate 24-well sitting drop protein crystallization trials. Using Python scripts for precise control, the study explores the robot's application in mixing and setting up crystallization plates with a model protein (hen egg white lysozyme) and a periplasmic protein from Campylobacter jejuni, a crystal utilized in the Snow lab as a biomaterial for nanotechnology that requires large, consistent batches. In a head-to-head comparison with manual 24-well plate setup, crystal growth statistics indicate our approach can reduce manual labor and increase reliability in protein crystallization, and may also reduce variability, offering an economical and versatile tool for laboratories. This study shows facile adaption of the Opentrons interface and hardware for growth of two different crystal types. All developed liquid handling routines and relevant data files, in addition to demonstration videos are available at https://github.com/jbderoo/Opentrons2-Protein-Crystallization |
| format | Article |
| id | doaj-art-de00d45ef6cb4701a60ef1dea25938df |
| institution | Kabale University |
| issn | 2472-6303 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | SLAS Technology |
| spelling | doaj-art-de00d45ef6cb4701a60ef1dea25938df2025-08-20T03:39:57ZengElsevierSLAS Technology2472-63032025-06-013210026810.1016/j.slast.2025.100268Automation of protein crystallization scaleup via Opentrons-2 liquid handlingJacob B. DeRoo0Alec A. Jones1Caroline K. Slaughter2Tim W. Ahr3Sam M. Stroup4Grace B. Thompson5Christopher D. Snow6School of Biomedical Engineering, Colorado State University, United States of AmericaSchool of Biomedical Engineering, Colorado State University, United States of AmericaDepartment of Cellular and Molecular Biology, Colorado State University, United States of AmericaDepartment of Chemical Engineering, Colorado State University, United States of AmericaDepartment of Chemistry, Colorado State University, United States of AmericaSchool of Biomedical Engineering, Colorado State University, United States of America; Department of Electrical Engineering, Colorado State University, United States of AmericaSchool of Biomedical Engineering, Colorado State University, United States of America; Department of Cellular and Molecular Biology, Colorado State University, United States of America; Department of Chemical Engineering, Colorado State University, United States of America; Department of Chemistry, Colorado State University, United States of America; Corresponding author.In this study we present an approach for optimizing protein crystallization trials at the multi-microliter scale utilizing the Opentrons-2 liquid handling robot. Our research demonstrates the robot's capability to automate 24-well sitting drop protein crystallization trials. Using Python scripts for precise control, the study explores the robot's application in mixing and setting up crystallization plates with a model protein (hen egg white lysozyme) and a periplasmic protein from Campylobacter jejuni, a crystal utilized in the Snow lab as a biomaterial for nanotechnology that requires large, consistent batches. In a head-to-head comparison with manual 24-well plate setup, crystal growth statistics indicate our approach can reduce manual labor and increase reliability in protein crystallization, and may also reduce variability, offering an economical and versatile tool for laboratories. This study shows facile adaption of the Opentrons interface and hardware for growth of two different crystal types. All developed liquid handling routines and relevant data files, in addition to demonstration videos are available at https://github.com/jbderoo/Opentrons2-Protein-Crystallizationhttp://www.sciencedirect.com/science/article/pii/S2472630325000263Liquid handlingProtein crystallizationCrystallographySitting drop crystallization |
| spellingShingle | Jacob B. DeRoo Alec A. Jones Caroline K. Slaughter Tim W. Ahr Sam M. Stroup Grace B. Thompson Christopher D. Snow Automation of protein crystallization scaleup via Opentrons-2 liquid handling SLAS Technology Liquid handling Protein crystallization Crystallography Sitting drop crystallization |
| title | Automation of protein crystallization scaleup via Opentrons-2 liquid handling |
| title_full | Automation of protein crystallization scaleup via Opentrons-2 liquid handling |
| title_fullStr | Automation of protein crystallization scaleup via Opentrons-2 liquid handling |
| title_full_unstemmed | Automation of protein crystallization scaleup via Opentrons-2 liquid handling |
| title_short | Automation of protein crystallization scaleup via Opentrons-2 liquid handling |
| title_sort | automation of protein crystallization scaleup via opentrons 2 liquid handling |
| topic | Liquid handling Protein crystallization Crystallography Sitting drop crystallization |
| url | http://www.sciencedirect.com/science/article/pii/S2472630325000263 |
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