Comparison of QIAstat-Dx and BioFire FilmArray Gastrointestinal Panels in a Pediatric Population

Accurate laboratory diagnosis of gastroenteritis is important to ensure that patients receive appropriate treatment and proper isolation precautions. This study evaluated the performance of the QIAGEN QIAstat-Dx gastrointestinal panel (QGP) in comparison to the bioMerieux BioFire FilmArray gastroint...

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Main Authors: Mohammed Suleiman, Muhammad Iqbal, Patrick Tang, Andrés Pérez-López
Format: Article
Language:English
Published: MDPI AG 2024-11-01
Series:Microorganisms
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Online Access:https://www.mdpi.com/2076-2607/12/11/2282
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author Mohammed Suleiman
Muhammad Iqbal
Patrick Tang
Andrés Pérez-López
author_facet Mohammed Suleiman
Muhammad Iqbal
Patrick Tang
Andrés Pérez-López
author_sort Mohammed Suleiman
collection DOAJ
description Accurate laboratory diagnosis of gastroenteritis is important to ensure that patients receive appropriate treatment and proper isolation precautions. This study evaluated the performance of the QIAGEN QIAstat-Dx gastrointestinal panel (QGP) in comparison to the bioMerieux BioFire FilmArray gastrointestinal panel (BGP) for the detection of gastrointestinal pathogens in 110 pediatric patients being evaluated for gastroenteritis at our hospital. We compared 23 different bacterial, viral, and parasite enteropathogens detected by the QGP against the BGP. The overall positive percent agreement (PPA) for all compared targets was 96.2% and the overall negative percent agreement (NPA) for all compared targets was 99.7%. Our study shows that QIAstat-Dx QGP provides comparable results to the BioFire BGP in our pediatric population. Additionally, the PCR cycle threshold (Ct) value reported by the QGP is potentially a helpful tool in estimating the load of the detected pathogen in stool samples.
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issn 2076-2607
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spelling doaj-art-ddf27ed846ff45efaebf948a34973f2d2025-08-20T02:48:01ZengMDPI AGMicroorganisms2076-26072024-11-011211228210.3390/microorganisms12112282Comparison of QIAstat-Dx and BioFire FilmArray Gastrointestinal Panels in a Pediatric PopulationMohammed Suleiman0Muhammad Iqbal1Patrick Tang2Andrés Pérez-López3Department of Pathology, Sidra Medicine, Doha P.O. Box 26999, QatarDepartment of Pathology, Sidra Medicine, Doha P.O. Box 26999, QatarDepartment of Pathology, Sidra Medicine, Doha P.O. Box 26999, QatarDepartment of Pathology, Sidra Medicine, Doha P.O. Box 26999, QatarAccurate laboratory diagnosis of gastroenteritis is important to ensure that patients receive appropriate treatment and proper isolation precautions. This study evaluated the performance of the QIAGEN QIAstat-Dx gastrointestinal panel (QGP) in comparison to the bioMerieux BioFire FilmArray gastrointestinal panel (BGP) for the detection of gastrointestinal pathogens in 110 pediatric patients being evaluated for gastroenteritis at our hospital. We compared 23 different bacterial, viral, and parasite enteropathogens detected by the QGP against the BGP. The overall positive percent agreement (PPA) for all compared targets was 96.2% and the overall negative percent agreement (NPA) for all compared targets was 99.7%. Our study shows that QIAstat-Dx QGP provides comparable results to the BioFire BGP in our pediatric population. Additionally, the PCR cycle threshold (Ct) value reported by the QGP is potentially a helpful tool in estimating the load of the detected pathogen in stool samples.https://www.mdpi.com/2076-2607/12/11/2282GI panelsyndromic testingQIAstatBioFire
spellingShingle Mohammed Suleiman
Muhammad Iqbal
Patrick Tang
Andrés Pérez-López
Comparison of QIAstat-Dx and BioFire FilmArray Gastrointestinal Panels in a Pediatric Population
Microorganisms
GI panel
syndromic testing
QIAstat
BioFire
title Comparison of QIAstat-Dx and BioFire FilmArray Gastrointestinal Panels in a Pediatric Population
title_full Comparison of QIAstat-Dx and BioFire FilmArray Gastrointestinal Panels in a Pediatric Population
title_fullStr Comparison of QIAstat-Dx and BioFire FilmArray Gastrointestinal Panels in a Pediatric Population
title_full_unstemmed Comparison of QIAstat-Dx and BioFire FilmArray Gastrointestinal Panels in a Pediatric Population
title_short Comparison of QIAstat-Dx and BioFire FilmArray Gastrointestinal Panels in a Pediatric Population
title_sort comparison of qiastat dx and biofire filmarray gastrointestinal panels in a pediatric population
topic GI panel
syndromic testing
QIAstat
BioFire
url https://www.mdpi.com/2076-2607/12/11/2282
work_keys_str_mv AT mohammedsuleiman comparisonofqiastatdxandbiofirefilmarraygastrointestinalpanelsinapediatricpopulation
AT muhammadiqbal comparisonofqiastatdxandbiofirefilmarraygastrointestinalpanelsinapediatricpopulation
AT patricktang comparisonofqiastatdxandbiofirefilmarraygastrointestinalpanelsinapediatricpopulation
AT andresperezlopez comparisonofqiastatdxandbiofirefilmarraygastrointestinalpanelsinapediatricpopulation