Kinetics model for enzymatic hydrolysis of eel protein
Kinetics model for enzymatic hydrolysis can serve as the theoretical basis for process control, and it helps to develop efficient procedures of bioactive peptides preparation through protein hydrolysis. The enzymatic hydrolysates from eel protein were demonstrated to be effective in anti-oxidation a...
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Zhejiang University Press
2013-03-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
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| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2012.11.618 |
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| author | ZHANG Zhiguo WANG Junhong CHEN Xinfeng ZHOU Ligen GAO Haiyan |
| author_facet | ZHANG Zhiguo WANG Junhong CHEN Xinfeng ZHOU Ligen GAO Haiyan |
| author_sort | ZHANG Zhiguo |
| collection | DOAJ |
| description | Kinetics model for enzymatic hydrolysis can serve as the theoretical basis for process control, and it helps to develop efficient procedures of bioactive peptides preparation through protein hydrolysis. The enzymatic hydrolysates from eel protein were demonstrated to be effective in anti-oxidation and inhibition of α-amylase in v itro, and could be exploited as resources of anti-diabetic drugs and healthy food. So far, no data on the kinetics model for enzymatic hydrolysis of eel protein can be found.To elucidate the kinetic characteristics of enzymatic hydrolysis of eel protein, the hydrolysis of eel protein at 50 ℃, pH=7.2, with different initial concentrations of the eel protein [S<sub>0</sub>] (0.25, 0.33, 0.50 g/mL) and different concentrations of the neutral protease [E<sub>0</sub>] (3.33, 6.67, 10.00, 13.33 mg/mL) was preformed. The degree of hydrolysis (DH) of the eel protein was determined during a 240-minute time course at varied time intervals. A kinetic model that took into account of enzyme inhibition by product or substrate was fit to the experimental data with nonlinear regression methods, and the parameters of the kinetic equation were estimated. The established kinetic equation was tested by comparing the predicted and actual DH values with paired t-test, when hydrolysis was conducted at 50 ℃, pH=7.2, and the initial concentrations of eel protein and neutral protease were 0.20 g/mL and 5.33 mg/mL, respectively.The results showed that the DH values increased rapidly within the first 30 minutes, after which the hydrolysis rate slowed down gradually. At 240 min, the DH values increased with the rise of the neutral protease concentrations, while decreased as the initial concentrations of the eel protein increased. The kinetic equation of eel protein hydrolysis was as follows: DH=1-(1+32.65[E<sub>0</sub>]/[S<sub>0</sub>]·t-0.1 t)-0.061 9. The critical concentration of the neutral protease was 3.06×10<sup>-3</sup> [S<sub>0</sub>], and the critical concentration of the eel protein was 326.50 [E<sub>0</sub>]. During the 240 min hydrolysis, no significant difference was found between the actual and predicted by this kinetic equation DH values.In conclusion, both substrate inhibition and product inhibition existed during the eel protein hydrolysis catalyzed by neutral protease. There are critical concentrations for substrate and protease. The enzymatic hydrolysis will be impeded when the initial concentration of the eel protein is higher than the critical concentration, or the neutral protease concentration is lower than the critical concentration. The kinetic equation can fit the experimental data well, and can be used to predict the eel protein hydrolysis with neutral protease. |
| format | Article |
| id | doaj-art-dde366f2104a40a39f1987d52a4b3c36 |
| institution | Kabale University |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2013-03-01 |
| publisher | Zhejiang University Press |
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| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-dde366f2104a40a39f1987d52a4b3c362025-08-20T03:58:18ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552013-03-013922723210.3785/j.issn.1008-9209.2012.11.61810089209Kinetics model for enzymatic hydrolysis of eel proteinZHANG ZhiguoWANG JunhongCHEN XinfengZHOU LigenGAO HaiyanKinetics model for enzymatic hydrolysis can serve as the theoretical basis for process control, and it helps to develop efficient procedures of bioactive peptides preparation through protein hydrolysis. The enzymatic hydrolysates from eel protein were demonstrated to be effective in anti-oxidation and inhibition of α-amylase in v itro, and could be exploited as resources of anti-diabetic drugs and healthy food. So far, no data on the kinetics model for enzymatic hydrolysis of eel protein can be found.To elucidate the kinetic characteristics of enzymatic hydrolysis of eel protein, the hydrolysis of eel protein at 50 ℃, pH=7.2, with different initial concentrations of the eel protein [S<sub>0</sub>] (0.25, 0.33, 0.50 g/mL) and different concentrations of the neutral protease [E<sub>0</sub>] (3.33, 6.67, 10.00, 13.33 mg/mL) was preformed. The degree of hydrolysis (DH) of the eel protein was determined during a 240-minute time course at varied time intervals. A kinetic model that took into account of enzyme inhibition by product or substrate was fit to the experimental data with nonlinear regression methods, and the parameters of the kinetic equation were estimated. The established kinetic equation was tested by comparing the predicted and actual DH values with paired t-test, when hydrolysis was conducted at 50 ℃, pH=7.2, and the initial concentrations of eel protein and neutral protease were 0.20 g/mL and 5.33 mg/mL, respectively.The results showed that the DH values increased rapidly within the first 30 minutes, after which the hydrolysis rate slowed down gradually. At 240 min, the DH values increased with the rise of the neutral protease concentrations, while decreased as the initial concentrations of the eel protein increased. The kinetic equation of eel protein hydrolysis was as follows: DH=1-(1+32.65[E<sub>0</sub>]/[S<sub>0</sub>]·t-0.1 t)-0.061 9. The critical concentration of the neutral protease was 3.06×10<sup>-3</sup> [S<sub>0</sub>], and the critical concentration of the eel protein was 326.50 [E<sub>0</sub>]. During the 240 min hydrolysis, no significant difference was found between the actual and predicted by this kinetic equation DH values.In conclusion, both substrate inhibition and product inhibition existed during the eel protein hydrolysis catalyzed by neutral protease. There are critical concentrations for substrate and protease. The enzymatic hydrolysis will be impeded when the initial concentration of the eel protein is higher than the critical concentration, or the neutral protease concentration is lower than the critical concentration. The kinetic equation can fit the experimental data well, and can be used to predict the eel protein hydrolysis with neutral protease.https://www.academax.com/doi/10.3785/j.issn.1008-9209.2012.11.618eel proteinneutral proteaseenzymatic hydrolysiskinetics |
| spellingShingle | ZHANG Zhiguo WANG Junhong CHEN Xinfeng ZHOU Ligen GAO Haiyan Kinetics model for enzymatic hydrolysis of eel protein 浙江大学学报. 农业与生命科学版 eel protein neutral protease enzymatic hydrolysis kinetics |
| title | Kinetics model for enzymatic hydrolysis of eel protein |
| title_full | Kinetics model for enzymatic hydrolysis of eel protein |
| title_fullStr | Kinetics model for enzymatic hydrolysis of eel protein |
| title_full_unstemmed | Kinetics model for enzymatic hydrolysis of eel protein |
| title_short | Kinetics model for enzymatic hydrolysis of eel protein |
| title_sort | kinetics model for enzymatic hydrolysis of eel protein |
| topic | eel protein neutral protease enzymatic hydrolysis kinetics |
| url | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2012.11.618 |
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