Phage Therapy Against Biofilm of Multidrug-Resistant Klebsiella Pneumoniae Isolated from Zakho Hospital Samples
Klebsiella pneumoniae causes infection in human, especially in immunocompromised patients. About 80% of nosocomial infection caused by K. pneumoniae is due to multidrug-resistant strain. The emergence of antibiotic-resistant bacterial strains necessitates the exploration of alternative antibacterial...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Erbil Polytechnic University
2019-06-01
|
| Series: | Polytechnic Journal |
| Subjects: | |
| Online Access: | https://polytechnic-journal.epu.edu.iq/home/vol9/iss1/5 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Klebsiella pneumoniae causes infection in human, especially in immunocompromised patients. About
80% of nosocomial infection caused by K. pneumoniae is due to multidrug-resistant strain. The
emergence of antibiotic-resistant bacterial strains necessitates the exploration of alternative antibacterial
therapies, which led to studying the ability of viruses that infect the bacteria (known as bacteriophage)
to treat infection with K. pneumoniae. Bacterial biofilm which are crucial in the pathogenesis of
much clinically important infection and are difficult to eradicate because they exist resistant to many
antimicrobial treatment. Biofilm formation by K. pneumoniae is responsible for the catheter associated
infection such as urinary tract infection and respiratory tract infection due to the colonization of the
polymeric surface by forming multilayered cell cluster embedded in extracellular materials. In this
study K. pneumoniae isolated from the hospital environment and characterized it and form the biofilm
of that organism by microplate quantitative assay. Similarly bacteriophage specific for K. pneumoniae
isolated from river water. The aim of work is the use of bacteriophage as a possible alternative for
the treatment of bacterial infection of K. pneumoniae. We showed that biofilm is reduced by isolated
phages by the comparative account of colony-forming unit versus plaque-forming unit. The result of
this study, therefore, suggests that the timing of starting the phage therapy after initiation of infection
significantly contributes toward the success of the treatment. |
|---|---|
| ISSN: | 2707-7799 |