Repurposing of Oxicam Derivatives to Inhibit NDM-1: Molecular Docking and Molecular Dynamic Simulation Studies

The New Delhi Metallo-β-lactamase-1 (NDM-1) causes hydrolysis of broad spectrum β-lactam antibiotics, such as carbapenems, resulting in the development of antimicrobial resistance. Still, there are not any approved NDM-1 inhibitors, globally. Therefore, repositioning approved medicines as NDM-1 inh...

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Main Authors: Pshtiwan Gharib Ali, Twana Mohsin Salih
Format: Article
Language:English
Published: College of Pharmacy / Mustansiriyah University 2024-10-01
Series:Al-Mustansiriyah Journal of Pharmaceutical Sciences
Subjects:
Online Access:https://ajps.uomustansiriyah.edu.iq/index.php/AJPS/article/view/1029
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author Pshtiwan Gharib Ali
Twana Mohsin Salih
author_facet Pshtiwan Gharib Ali
Twana Mohsin Salih
author_sort Pshtiwan Gharib Ali
collection DOAJ
description The New Delhi Metallo-β-lactamase-1 (NDM-1) causes hydrolysis of broad spectrum β-lactam antibiotics, such as carbapenems, resulting in the development of antimicrobial resistance. Still, there are not any approved NDM-1 inhibitors, globally. Therefore, repositioning approved medicines as NDM-1 inhibitors to combine with carbapenems may be a crucial strategy to combat resistant pathogens. This study repurposes. Oxicam derivatives as inhibitors of bacterial NDM-1. The two-dimensional structures were obtained from the PubChem database. Twenty derivatives of oxicam were assessed computationally to realize their NDM-1 inhibition capability.  To identify potential inhibitors of the NDM-1 target protein, a molecular docking protocol was used. In addition, drug-likeness and pharmacokinetic properties were predicted for the designed molecules. Three compounds with the most negative ΔGbinding results were chosen for additional study using molecular dynamic (MD) simulations. The compounds M010, M013, and M016 possessed a significantly more negative binding free energy than the positive control and other designed molecules, had stable MD simulations (Root-mean-square deviation < 0.5 Å), passed Lipinski's rule of five, and possessed favourable physicochemical and pharmacokinetic properties. The findings can inform In vitro studies of the promising compounds.
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spelling doaj-art-ddd1ed622db64ca880401f7cb03d08cd2025-08-20T02:09:11ZengCollege of Pharmacy / Mustansiriyah UniversityAl-Mustansiriyah Journal of Pharmaceutical Sciences1815-09932959-183X2024-10-0124410.32947/ajps.v24i4.1029Repurposing of Oxicam Derivatives to Inhibit NDM-1: Molecular Docking and Molecular Dynamic Simulation StudiesPshtiwan Gharib Ali0Twana Mohsin Salih1Department of Pharmacognosy & Pharmaceutical Chemistry, College of Pharmacy, University of Sulaimani, IraqDepartment of Pharmacognosy & Pharmaceutical Chemistry, College of Pharmacy, University of Sulaimani, Iraq The New Delhi Metallo-β-lactamase-1 (NDM-1) causes hydrolysis of broad spectrum β-lactam antibiotics, such as carbapenems, resulting in the development of antimicrobial resistance. Still, there are not any approved NDM-1 inhibitors, globally. Therefore, repositioning approved medicines as NDM-1 inhibitors to combine with carbapenems may be a crucial strategy to combat resistant pathogens. This study repurposes. Oxicam derivatives as inhibitors of bacterial NDM-1. The two-dimensional structures were obtained from the PubChem database. Twenty derivatives of oxicam were assessed computationally to realize their NDM-1 inhibition capability.  To identify potential inhibitors of the NDM-1 target protein, a molecular docking protocol was used. In addition, drug-likeness and pharmacokinetic properties were predicted for the designed molecules. Three compounds with the most negative ΔGbinding results were chosen for additional study using molecular dynamic (MD) simulations. The compounds M010, M013, and M016 possessed a significantly more negative binding free energy than the positive control and other designed molecules, had stable MD simulations (Root-mean-square deviation < 0.5 Å), passed Lipinski's rule of five, and possessed favourable physicochemical and pharmacokinetic properties. The findings can inform In vitro studies of the promising compounds. https://ajps.uomustansiriyah.edu.iq/index.php/AJPS/article/view/1029Antibiotic resistancedrug repurposingmolecular dockingmolecular dynamics simulationNew Delhi Metallo-β-lactamase-1oxicam derivatives
spellingShingle Pshtiwan Gharib Ali
Twana Mohsin Salih
Repurposing of Oxicam Derivatives to Inhibit NDM-1: Molecular Docking and Molecular Dynamic Simulation Studies
Al-Mustansiriyah Journal of Pharmaceutical Sciences
Antibiotic resistance
drug repurposing
molecular docking
molecular dynamics simulation
New Delhi Metallo-β-lactamase-1
oxicam derivatives
title Repurposing of Oxicam Derivatives to Inhibit NDM-1: Molecular Docking and Molecular Dynamic Simulation Studies
title_full Repurposing of Oxicam Derivatives to Inhibit NDM-1: Molecular Docking and Molecular Dynamic Simulation Studies
title_fullStr Repurposing of Oxicam Derivatives to Inhibit NDM-1: Molecular Docking and Molecular Dynamic Simulation Studies
title_full_unstemmed Repurposing of Oxicam Derivatives to Inhibit NDM-1: Molecular Docking and Molecular Dynamic Simulation Studies
title_short Repurposing of Oxicam Derivatives to Inhibit NDM-1: Molecular Docking and Molecular Dynamic Simulation Studies
title_sort repurposing of oxicam derivatives to inhibit ndm 1 molecular docking and molecular dynamic simulation studies
topic Antibiotic resistance
drug repurposing
molecular docking
molecular dynamics simulation
New Delhi Metallo-β-lactamase-1
oxicam derivatives
url https://ajps.uomustansiriyah.edu.iq/index.php/AJPS/article/view/1029
work_keys_str_mv AT pshtiwangharibali repurposingofoxicamderivativestoinhibitndm1moleculardockingandmoleculardynamicsimulationstudies
AT twanamohsinsalih repurposingofoxicamderivativestoinhibitndm1moleculardockingandmoleculardynamicsimulationstudies