Plasma Lipidomics of HIV-1 infected patients based on ultra performance liquid chromatography–mass spectrometry

Acquired immune deficiency syndrome (AIDS) has been shown to not only attack the body's immune system but also to cause metabolic disorders. Highly active anti-retroviral therapy (HAART) is the main treatment for AIDS, but it can also affect the human metabolic system and cause metabolic disord...

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Main Authors: Aoxin Ma, Zhaobo Wu, Guojun Li, Xinyu Zhang, Xiangyun Tang, Shuofan Dong, Xinli Lu, Kaoqi Lian
Format: Article
Language:English
Published: Elsevier 2025-05-01
Series:Results in Chemistry
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Online Access:http://www.sciencedirect.com/science/article/pii/S2211715625002723
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author Aoxin Ma
Zhaobo Wu
Guojun Li
Xinyu Zhang
Xiangyun Tang
Shuofan Dong
Xinli Lu
Kaoqi Lian
author_facet Aoxin Ma
Zhaobo Wu
Guojun Li
Xinyu Zhang
Xiangyun Tang
Shuofan Dong
Xinli Lu
Kaoqi Lian
author_sort Aoxin Ma
collection DOAJ
description Acquired immune deficiency syndrome (AIDS) has been shown to not only attack the body's immune system but also to cause metabolic disorders. Highly active anti-retroviral therapy (HAART) is the main treatment for AIDS, but it can also affect the human metabolic system and cause metabolic disorders. In this study, we analyzed how AIDS and HAART alter human metabolism through an ultra performance liquid chromatography–mass spectrometry (UPLC-MS) lipidomics approach. Differential metabolites with significant changes were identified by multivariate statistical analysis. There were 44 significantly different metabolites detected in the healthy volunteers and HAART-naïve patients, and 61 significantly different metabolites detected in the healthy volunteers and patients under HAART. Screening for biomarkers based on the area under the receiver operating characteristic curve. In the healthy and untreated groups, Sphinganine was identified as potential biomarkers. In the healthy and treated groups, Psychosine and Sphinganine were identified as potential biomarkers. Sphinganine and Psychosine as biomarkers have a wide range of potential clinical applications. In psychiatric disorders and metabolic disorders they can provide new directions for early diagnosis, therapeutic monitoring, and prognostic assessment of diseases. Future studies will further explore the specific mechanisms and clinical application value of these biomarkers.
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spelling doaj-art-ddd1556e9e994d5ebe39f34e589a63162025-08-20T02:33:12ZengElsevierResults in Chemistry2211-71562025-05-011510228910.1016/j.rechem.2025.102289Plasma Lipidomics of HIV-1 infected patients based on ultra performance liquid chromatography–mass spectrometryAoxin Ma0Zhaobo Wu1Guojun Li2Xinyu Zhang3Xiangyun Tang4Shuofan Dong5Xinli Lu6Kaoqi Lian7School of Public Health, Hebei Medical University, Shijiazhuang 050017, ChinaSchool of Public Health, Hebei Medical University, Shijiazhuang 050017, ChinaHebei Research Institute of Sports Science, Shijiazhuang 050011, ChinaSchool of Public Health, Hebei Medical University, Shijiazhuang 050017, ChinaCollege of Public Health, North China University of Science and Technology, Tangshan, ChinaSchool of Public Health, Hebei Medical University, Shijiazhuang 050017, ChinaSchool of Public Health, Hebei Medical University, Shijiazhuang 050017, China; Department of AIDS Research, Hebei Key Laboratory of Pathogen and Epidemiology of Infectious Disease, Hebei Provincial Center for Disease Control and Prevention, Shijiazhuang, China; Corresponding author: Department of AIDS Research, Hebei Provincial Center for Disease Control and Prevention, No. 97 Huai'an East Road, Yuhua Distinct, Shijiazhuang, Hebei 050021, China.School of Public Health, Hebei Medical University, Shijiazhuang 050017, China; Hebei Key Laboratory of Early Life Health Promotion, Hebei Province, Shijiazhuang 050017, China; Hebei Key Laboratory of Environment and Human Health, Shijiazhuang 050017, China; Corresponding author: School of Public Health, Hebei Medical University, Shijiazhuang, China.Acquired immune deficiency syndrome (AIDS) has been shown to not only attack the body's immune system but also to cause metabolic disorders. Highly active anti-retroviral therapy (HAART) is the main treatment for AIDS, but it can also affect the human metabolic system and cause metabolic disorders. In this study, we analyzed how AIDS and HAART alter human metabolism through an ultra performance liquid chromatography–mass spectrometry (UPLC-MS) lipidomics approach. Differential metabolites with significant changes were identified by multivariate statistical analysis. There were 44 significantly different metabolites detected in the healthy volunteers and HAART-naïve patients, and 61 significantly different metabolites detected in the healthy volunteers and patients under HAART. Screening for biomarkers based on the area under the receiver operating characteristic curve. In the healthy and untreated groups, Sphinganine was identified as potential biomarkers. In the healthy and treated groups, Psychosine and Sphinganine were identified as potential biomarkers. Sphinganine and Psychosine as biomarkers have a wide range of potential clinical applications. In psychiatric disorders and metabolic disorders they can provide new directions for early diagnosis, therapeutic monitoring, and prognostic assessment of diseases. Future studies will further explore the specific mechanisms and clinical application value of these biomarkers.http://www.sciencedirect.com/science/article/pii/S2211715625002723BiomarkersHIVUPLC-MSLipidomicsMetabolomics
spellingShingle Aoxin Ma
Zhaobo Wu
Guojun Li
Xinyu Zhang
Xiangyun Tang
Shuofan Dong
Xinli Lu
Kaoqi Lian
Plasma Lipidomics of HIV-1 infected patients based on ultra performance liquid chromatography–mass spectrometry
Results in Chemistry
Biomarkers
HIV
UPLC-MS
Lipidomics
Metabolomics
title Plasma Lipidomics of HIV-1 infected patients based on ultra performance liquid chromatography–mass spectrometry
title_full Plasma Lipidomics of HIV-1 infected patients based on ultra performance liquid chromatography–mass spectrometry
title_fullStr Plasma Lipidomics of HIV-1 infected patients based on ultra performance liquid chromatography–mass spectrometry
title_full_unstemmed Plasma Lipidomics of HIV-1 infected patients based on ultra performance liquid chromatography–mass spectrometry
title_short Plasma Lipidomics of HIV-1 infected patients based on ultra performance liquid chromatography–mass spectrometry
title_sort plasma lipidomics of hiv 1 infected patients based on ultra performance liquid chromatography mass spectrometry
topic Biomarkers
HIV
UPLC-MS
Lipidomics
Metabolomics
url http://www.sciencedirect.com/science/article/pii/S2211715625002723
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