Inhibition of Progenitor Dendritic Cell Maturation by Plasma from Patients with Peripartum Cardiomyopathy: Role in Pregnancy-associated Heart Disease
Dendritic cells (DCs) play dual roles in innate and adaptive immunity based on their functional maturity, and both innate and adaptive immune responses have been implicated in myocardial tissue remodeling associated with cardiomyopathies. Peripartum cardiomyopa...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2005-01-01
|
| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1080/17402520500304352 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850166064154935296 |
|---|---|
| author | Jane E. Ellis Aftab A. Ansari James D. Fett Robert D. Carraway Hugh W. Randall Mario I. Mosunjac J. Bruce Sundstrom |
| author_facet | Jane E. Ellis Aftab A. Ansari James D. Fett Robert D. Carraway Hugh W. Randall Mario I. Mosunjac J. Bruce Sundstrom |
| author_sort | Jane E. Ellis |
| collection | DOAJ |
| description | Dendritic cells (DCs) play dual roles in innate and adaptive immunity based
on their functional maturity, and both innate and adaptive immune responses have
been implicated in myocardial tissue remodeling associated with
cardiomyopathies. Peripartum cardiomyopathy (PPCM) is a rare disorder which
affects women within one month antepartum to five months postpartum. A high
occurrence of PPCM in central Haiti (1 in 300 live births) provided the unique
opportunity to study the relationship of immune activation and DC maturation
to the etiology of this disorder. Plasma samples from two groups (n = 12) of
age- and parity-matched Haitian women with or without evidence of PPCM were
tested for levels of biomarkers of cardiac tissue remodeling and immune
activation. Significantly elevated levels of GM-CSF, endothelin-1, proBNP and
CRP and decreased levels of TGF- were measured in PPCM subjects relative
to controls. Yet despite these findings, in vitro maturation of normal human
cord blood derived progenitor dendritic cells (CBDCs) was significantly
reduced (p < 0.001) in the presence of plasma from PPCM patients relative
to plasma from post-partum control subjects as determined by expression of
CD80, CD86, CD83, CCR7, MHC class II and the ability of these matured CBDCs
to induce allo-responses in PBMCs. These results represent the first findings
linking inhibition of DC maturation to the dysregulation of normal physiologic
cardiac
tissue remodeling during pregnancy and the pathogenesis of PPCM. |
| format | Article |
| id | doaj-art-ddd03f40a40e4d6eaefb968273f20c61 |
| institution | OA Journals |
| issn | 1740-2522 1740-2530 |
| language | English |
| publishDate | 2005-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Developmental Immunology |
| spelling | doaj-art-ddd03f40a40e4d6eaefb968273f20c612025-08-20T02:21:34ZengWileyClinical and Developmental Immunology1740-25221740-25302005-01-0112426527310.1080/17402520500304352Inhibition of Progenitor Dendritic Cell Maturation by Plasma from Patients with Peripartum Cardiomyopathy: Role in Pregnancy-associated Heart DiseaseJane E. Ellis0Aftab A. Ansari1James D. Fett2Robert D. Carraway3Hugh W. Randall4Mario I. Mosunjac5J. Bruce Sundstrom6Department of Gynecology and Obstetrics, Emory University School of Medicine, Atlanta 30322, GA, USADepartment of Pathology and Laboratory Medicine, Department of Pathology and Laboratory Medicine, Atlanta 30322, GA, USADepartment of Adult Medicine, Deschapelles, Hospital Albert Schweitzer, HaitiDepartment of Adult Medicine, Deschapelles, Hospital Albert Schweitzer, HaitiDepartment of Gynecology and Obstetrics, Emory University School of Medicine, Atlanta 30322, GA, USADepartment of Pathology and Laboratory Medicine, Department of Pathology and Laboratory Medicine, Atlanta 30322, GA, USADepartment of Pathology and Laboratory Medicine, Department of Pathology and Laboratory Medicine, Atlanta 30322, GA, USADendritic cells (DCs) play dual roles in innate and adaptive immunity based on their functional maturity, and both innate and adaptive immune responses have been implicated in myocardial tissue remodeling associated with cardiomyopathies. Peripartum cardiomyopathy (PPCM) is a rare disorder which affects women within one month antepartum to five months postpartum. A high occurrence of PPCM in central Haiti (1 in 300 live births) provided the unique opportunity to study the relationship of immune activation and DC maturation to the etiology of this disorder. Plasma samples from two groups (n = 12) of age- and parity-matched Haitian women with or without evidence of PPCM were tested for levels of biomarkers of cardiac tissue remodeling and immune activation. Significantly elevated levels of GM-CSF, endothelin-1, proBNP and CRP and decreased levels of TGF- were measured in PPCM subjects relative to controls. Yet despite these findings, in vitro maturation of normal human cord blood derived progenitor dendritic cells (CBDCs) was significantly reduced (p < 0.001) in the presence of plasma from PPCM patients relative to plasma from post-partum control subjects as determined by expression of CD80, CD86, CD83, CCR7, MHC class II and the ability of these matured CBDCs to induce allo-responses in PBMCs. These results represent the first findings linking inhibition of DC maturation to the dysregulation of normal physiologic cardiac tissue remodeling during pregnancy and the pathogenesis of PPCM.http://dx.doi.org/10.1080/17402520500304352 |
| spellingShingle | Jane E. Ellis Aftab A. Ansari James D. Fett Robert D. Carraway Hugh W. Randall Mario I. Mosunjac J. Bruce Sundstrom Inhibition of Progenitor Dendritic Cell Maturation by Plasma from Patients with Peripartum Cardiomyopathy: Role in Pregnancy-associated Heart Disease Clinical and Developmental Immunology |
| title | Inhibition of Progenitor Dendritic Cell Maturation by Plasma from Patients with Peripartum Cardiomyopathy: Role in Pregnancy-associated Heart Disease |
| title_full | Inhibition of Progenitor Dendritic Cell Maturation by Plasma from Patients with Peripartum Cardiomyopathy: Role in Pregnancy-associated Heart Disease |
| title_fullStr | Inhibition of Progenitor Dendritic Cell Maturation by Plasma from Patients with Peripartum Cardiomyopathy: Role in Pregnancy-associated Heart Disease |
| title_full_unstemmed | Inhibition of Progenitor Dendritic Cell Maturation by Plasma from Patients with Peripartum Cardiomyopathy: Role in Pregnancy-associated Heart Disease |
| title_short | Inhibition of Progenitor Dendritic Cell Maturation by Plasma from Patients with Peripartum Cardiomyopathy: Role in Pregnancy-associated Heart Disease |
| title_sort | inhibition of progenitor dendritic cell maturation by plasma from patients with peripartum cardiomyopathy role in pregnancy associated heart disease |
| url | http://dx.doi.org/10.1080/17402520500304352 |
| work_keys_str_mv | AT janeeellis inhibitionofprogenitordendriticcellmaturationbyplasmafrompatientswithperipartumcardiomyopathyroleinpregnancyassociatedheartdisease AT aftabaansari inhibitionofprogenitordendriticcellmaturationbyplasmafrompatientswithperipartumcardiomyopathyroleinpregnancyassociatedheartdisease AT jamesdfett inhibitionofprogenitordendriticcellmaturationbyplasmafrompatientswithperipartumcardiomyopathyroleinpregnancyassociatedheartdisease AT robertdcarraway inhibitionofprogenitordendriticcellmaturationbyplasmafrompatientswithperipartumcardiomyopathyroleinpregnancyassociatedheartdisease AT hughwrandall inhibitionofprogenitordendriticcellmaturationbyplasmafrompatientswithperipartumcardiomyopathyroleinpregnancyassociatedheartdisease AT marioimosunjac inhibitionofprogenitordendriticcellmaturationbyplasmafrompatientswithperipartumcardiomyopathyroleinpregnancyassociatedheartdisease AT jbrucesundstrom inhibitionofprogenitordendriticcellmaturationbyplasmafrompatientswithperipartumcardiomyopathyroleinpregnancyassociatedheartdisease |