Effects of Bone Morphogenetic Protein‐7 on Steroid‐Induced Extracellular Matrix Accumulation in Human Trabecular Meshwork Cells
ABSTRACT Long‐term steroid use, though essential for treating eye diseases, can cause increased intraocular pressure (IOP) in susceptible individuals and may lead to steroid‐induced glaucoma in a subset of patients. This study investigated the effect of bone morphogenetic protein‐7 (BMP‐7) on steroi...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2025-06-01
|
| Series: | FASEB BioAdvances |
| Subjects: | |
| Online Access: | https://doi.org/10.1096/fba.2025-00080 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850215468324880384 |
|---|---|
| author | Eun Woo Kim Jin‐Ok Choi Min Kyung Chae Jin‐Sol Lee Chang Ha Lee Jo Eun Um Nam Hee Kim Jihyeong Kim Wungrak Choi Chan Yun Kim |
| author_facet | Eun Woo Kim Jin‐Ok Choi Min Kyung Chae Jin‐Sol Lee Chang Ha Lee Jo Eun Um Nam Hee Kim Jihyeong Kim Wungrak Choi Chan Yun Kim |
| author_sort | Eun Woo Kim |
| collection | DOAJ |
| description | ABSTRACT Long‐term steroid use, though essential for treating eye diseases, can cause increased intraocular pressure (IOP) in susceptible individuals and may lead to steroid‐induced glaucoma in a subset of patients. This study investigated the effect of bone morphogenetic protein‐7 (BMP‐7) on steroid‐induced extracellular matrix (ECM) synthesis in human trabecular meshwork (TM) cells. We sought to explore the potential of BMP‐7 as a protective agent against steroid‐induced ECM accumulation in the TM. Human TM cells (HTMCs) were treated with either steroids alone or a combination of steroids and BMP‐7 to compare their effects on ECM production. BMP‐7, known for its transforming growth factor beta (TGF‐β) antagonistic properties, was administered using a micellized protein transduction domain (mPTD)‐fused BMP‐7 polypeptide to enhance activity. Gene expression analysis was conducted to identify specific genes involved in ECM regulation. BMP‐7 effectively inhibited steroid‐induced ECM accumulation in HTMCs. There was a significant reduction in ECM production in the steroid and BMP‐7 co‐treated group compared with that in the steroid‐only group. Furthermore, several genes involved in ECM regulation were identified in the co‐treatment, underscoring BMP‐7's potential role in modulating ECM metabolism. These findings demonstrate that BMP‐7 exerts protective, anti‐fibrotic effects in HTMCs by inhibiting steroid‐induced ECM synthesis. BMP‐7 may serve as a promising therapeutic target for preventing or treating steroid‐induced glaucoma by maintaining normal aqueous humor outflow and preventing IOP elevation. |
| format | Article |
| id | doaj-art-ddc864e5c50f4ee7950c86e2fc5f61a9 |
| institution | OA Journals |
| issn | 2573-9832 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | FASEB BioAdvances |
| spelling | doaj-art-ddc864e5c50f4ee7950c86e2fc5f61a92025-08-20T02:08:36ZengWileyFASEB BioAdvances2573-98322025-06-0176n/an/a10.1096/fba.2025-00080Effects of Bone Morphogenetic Protein‐7 on Steroid‐Induced Extracellular Matrix Accumulation in Human Trabecular Meshwork CellsEun Woo Kim0Jin‐Ok Choi1Min Kyung Chae2Jin‐Sol Lee3Chang Ha Lee4Jo Eun Um5Nam Hee Kim6Jihyeong Kim7Wungrak Choi8Chan Yun Kim9Department of Ophthalmology, Institute of Vision Research Yonsei University College of Medicine Seoul Republic of KoreaDepartment of Ophthalmology, Institute of Vision Research Yonsei University College of Medicine Seoul Republic of KoreaDepartment of Ophthalmology, Institute of Vision Research Yonsei University College of Medicine Seoul Republic of KoreaDivision of AI and Data Analysis Neurogrin Inc. Seoul Republic of KoreaDepartment of Ophthalmology, Institute of Vision Research Yonsei University College of Medicine Seoul Republic of KoreaMET Life Science Seoul Republic of KoreaOral Cancer Research Institute Yonsei University College of Dentistry Seoul Republic of KoreaDepartment of Ophthalmology, Institute of Vision Research Yonsei University College of Medicine Seoul Republic of KoreaDepartment of Ophthalmology, Institute of Vision Research Yonsei University College of Medicine Seoul Republic of KoreaDepartment of Ophthalmology, Institute of Vision Research Yonsei University College of Medicine Seoul Republic of KoreaABSTRACT Long‐term steroid use, though essential for treating eye diseases, can cause increased intraocular pressure (IOP) in susceptible individuals and may lead to steroid‐induced glaucoma in a subset of patients. This study investigated the effect of bone morphogenetic protein‐7 (BMP‐7) on steroid‐induced extracellular matrix (ECM) synthesis in human trabecular meshwork (TM) cells. We sought to explore the potential of BMP‐7 as a protective agent against steroid‐induced ECM accumulation in the TM. Human TM cells (HTMCs) were treated with either steroids alone or a combination of steroids and BMP‐7 to compare their effects on ECM production. BMP‐7, known for its transforming growth factor beta (TGF‐β) antagonistic properties, was administered using a micellized protein transduction domain (mPTD)‐fused BMP‐7 polypeptide to enhance activity. Gene expression analysis was conducted to identify specific genes involved in ECM regulation. BMP‐7 effectively inhibited steroid‐induced ECM accumulation in HTMCs. There was a significant reduction in ECM production in the steroid and BMP‐7 co‐treated group compared with that in the steroid‐only group. Furthermore, several genes involved in ECM regulation were identified in the co‐treatment, underscoring BMP‐7's potential role in modulating ECM metabolism. These findings demonstrate that BMP‐7 exerts protective, anti‐fibrotic effects in HTMCs by inhibiting steroid‐induced ECM synthesis. BMP‐7 may serve as a promising therapeutic target for preventing or treating steroid‐induced glaucoma by maintaining normal aqueous humor outflow and preventing IOP elevation.https://doi.org/10.1096/fba.2025-00080bone morphogenetic protein‐7extracellular matrixfibrosisglaucomasteroidtrabecular meshwork |
| spellingShingle | Eun Woo Kim Jin‐Ok Choi Min Kyung Chae Jin‐Sol Lee Chang Ha Lee Jo Eun Um Nam Hee Kim Jihyeong Kim Wungrak Choi Chan Yun Kim Effects of Bone Morphogenetic Protein‐7 on Steroid‐Induced Extracellular Matrix Accumulation in Human Trabecular Meshwork Cells FASEB BioAdvances bone morphogenetic protein‐7 extracellular matrix fibrosis glaucoma steroid trabecular meshwork |
| title | Effects of Bone Morphogenetic Protein‐7 on Steroid‐Induced Extracellular Matrix Accumulation in Human Trabecular Meshwork Cells |
| title_full | Effects of Bone Morphogenetic Protein‐7 on Steroid‐Induced Extracellular Matrix Accumulation in Human Trabecular Meshwork Cells |
| title_fullStr | Effects of Bone Morphogenetic Protein‐7 on Steroid‐Induced Extracellular Matrix Accumulation in Human Trabecular Meshwork Cells |
| title_full_unstemmed | Effects of Bone Morphogenetic Protein‐7 on Steroid‐Induced Extracellular Matrix Accumulation in Human Trabecular Meshwork Cells |
| title_short | Effects of Bone Morphogenetic Protein‐7 on Steroid‐Induced Extracellular Matrix Accumulation in Human Trabecular Meshwork Cells |
| title_sort | effects of bone morphogenetic protein 7 on steroid induced extracellular matrix accumulation in human trabecular meshwork cells |
| topic | bone morphogenetic protein‐7 extracellular matrix fibrosis glaucoma steroid trabecular meshwork |
| url | https://doi.org/10.1096/fba.2025-00080 |
| work_keys_str_mv | AT eunwookim effectsofbonemorphogeneticprotein7onsteroidinducedextracellularmatrixaccumulationinhumantrabecularmeshworkcells AT jinokchoi effectsofbonemorphogeneticprotein7onsteroidinducedextracellularmatrixaccumulationinhumantrabecularmeshworkcells AT minkyungchae effectsofbonemorphogeneticprotein7onsteroidinducedextracellularmatrixaccumulationinhumantrabecularmeshworkcells AT jinsollee effectsofbonemorphogeneticprotein7onsteroidinducedextracellularmatrixaccumulationinhumantrabecularmeshworkcells AT changhalee effectsofbonemorphogeneticprotein7onsteroidinducedextracellularmatrixaccumulationinhumantrabecularmeshworkcells AT joeunum effectsofbonemorphogeneticprotein7onsteroidinducedextracellularmatrixaccumulationinhumantrabecularmeshworkcells AT namheekim effectsofbonemorphogeneticprotein7onsteroidinducedextracellularmatrixaccumulationinhumantrabecularmeshworkcells AT jihyeongkim effectsofbonemorphogeneticprotein7onsteroidinducedextracellularmatrixaccumulationinhumantrabecularmeshworkcells AT wungrakchoi effectsofbonemorphogeneticprotein7onsteroidinducedextracellularmatrixaccumulationinhumantrabecularmeshworkcells AT chanyunkim effectsofbonemorphogeneticprotein7onsteroidinducedextracellularmatrixaccumulationinhumantrabecularmeshworkcells |