Investigation of Antiproliferative Effects of Home-Made and Commercial Apple Vinegars on Myeloma Cells

Vinegar is an aqueous food product made by a succession of yeast and acetic acid bacteria activities from fruits that contain high carbohydrates such as apples and grapes. Vinegar has been used as a dietary spice and natural remedy since ancient times due to its therapeutic properties including anti...

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Main Authors: Muhammet Mükerrem Kaya, Soner Tutun, Melike Sultan Usluer, Hidayet Tutun
Format: Article
Language:English
Published: Hasan Eleroğlu 2021-12-01
Series:Turkish Journal of Agriculture: Food Science and Technology
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Online Access:http://www.agrifoodscience.com/index.php/TURJAF/article/view/4416
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author Muhammet Mükerrem Kaya
Soner Tutun
Melike Sultan Usluer
Hidayet Tutun
author_facet Muhammet Mükerrem Kaya
Soner Tutun
Melike Sultan Usluer
Hidayet Tutun
author_sort Muhammet Mükerrem Kaya
collection DOAJ
description Vinegar is an aqueous food product made by a succession of yeast and acetic acid bacteria activities from fruits that contain high carbohydrates such as apples and grapes. Vinegar has been used as a dietary spice and natural remedy since ancient times due to its therapeutic properties including antimicrobial, antidiabetic, and anticancer activities. It has been shown that some bioactive compounds exhibiting antioxidant activity in vinegars lead to anticancer activity. The aim of the present study was to investigate antiproliferative effect of commercial and home-made apple vinegars in native and neutralized form on myeloma cells. In order to neutralize the vinegars, sodium hydroxide (NaOH) was used. A serial two-fold dilutions of the vinegars (50%, 25%, 12.5%, 6.25%, 3.12%, 1.56%, 0.78%, 0.39%) prepared with cell medium were treated to the cells. The MTT (3-(4.5-Dimethylthiazol-2-yl)-2.5-Diphenyltetrazolium Bromide) assay was used to determine the cellular viability in the cells treated with the vinegars. In this study, while commercial vinegar possessed a stronger antiproliferative activity than home-made vinegar, all native vinegars possessed stronger antiproliferative effect than neutralized vinegars. Interestingly, when home-made vinegar (both native and neutralized) concentrations were from 6.25 to 1.56%, the cell viability increased. Apple vinegar exhibited antiproliferative activity on myeloma cells; however, further studies are required to clarify the mechanisms underlying this activity.
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publishDate 2021-12-01
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series Turkish Journal of Agriculture: Food Science and Technology
spelling doaj-art-ddc7f35d66354e2cb33fa421e4c6c1f12025-08-20T03:25:06ZengHasan EleroğluTurkish Journal of Agriculture: Food Science and Technology2148-127X2021-12-019122173217810.24925/turjaf.v9i12.2173-2178.44162271Investigation of Antiproliferative Effects of Home-Made and Commercial Apple Vinegars on Myeloma CellsMuhammet Mükerrem Kaya0Soner Tutun1Melike Sultan Usluer2Hidayet Tutun3Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Burdur Mehmet Akif Ersoy University, BurdurDepartment of Food Hygiene and Technology, Faculty of Veterinary Medicine, Burdur Mehmet Akif Ersoy University, BurdurDepartment of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Burdur Mehmet Akif Ersoy University, BurdurDepartment of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Burdur Mehmet Akif Ersoy University, BurdurVinegar is an aqueous food product made by a succession of yeast and acetic acid bacteria activities from fruits that contain high carbohydrates such as apples and grapes. Vinegar has been used as a dietary spice and natural remedy since ancient times due to its therapeutic properties including antimicrobial, antidiabetic, and anticancer activities. It has been shown that some bioactive compounds exhibiting antioxidant activity in vinegars lead to anticancer activity. The aim of the present study was to investigate antiproliferative effect of commercial and home-made apple vinegars in native and neutralized form on myeloma cells. In order to neutralize the vinegars, sodium hydroxide (NaOH) was used. A serial two-fold dilutions of the vinegars (50%, 25%, 12.5%, 6.25%, 3.12%, 1.56%, 0.78%, 0.39%) prepared with cell medium were treated to the cells. The MTT (3-(4.5-Dimethylthiazol-2-yl)-2.5-Diphenyltetrazolium Bromide) assay was used to determine the cellular viability in the cells treated with the vinegars. In this study, while commercial vinegar possessed a stronger antiproliferative activity than home-made vinegar, all native vinegars possessed stronger antiproliferative effect than neutralized vinegars. Interestingly, when home-made vinegar (both native and neutralized) concentrations were from 6.25 to 1.56%, the cell viability increased. Apple vinegar exhibited antiproliferative activity on myeloma cells; however, further studies are required to clarify the mechanisms underlying this activity.http://www.agrifoodscience.com/index.php/TURJAF/article/view/4416antiproliferative activityapplemyelomavinegar
spellingShingle Muhammet Mükerrem Kaya
Soner Tutun
Melike Sultan Usluer
Hidayet Tutun
Investigation of Antiproliferative Effects of Home-Made and Commercial Apple Vinegars on Myeloma Cells
Turkish Journal of Agriculture: Food Science and Technology
antiproliferative activity
apple
myeloma
vinegar
title Investigation of Antiproliferative Effects of Home-Made and Commercial Apple Vinegars on Myeloma Cells
title_full Investigation of Antiproliferative Effects of Home-Made and Commercial Apple Vinegars on Myeloma Cells
title_fullStr Investigation of Antiproliferative Effects of Home-Made and Commercial Apple Vinegars on Myeloma Cells
title_full_unstemmed Investigation of Antiproliferative Effects of Home-Made and Commercial Apple Vinegars on Myeloma Cells
title_short Investigation of Antiproliferative Effects of Home-Made and Commercial Apple Vinegars on Myeloma Cells
title_sort investigation of antiproliferative effects of home made and commercial apple vinegars on myeloma cells
topic antiproliferative activity
apple
myeloma
vinegar
url http://www.agrifoodscience.com/index.php/TURJAF/article/view/4416
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AT melikesultanusluer investigationofantiproliferativeeffectsofhomemadeandcommercialapplevinegarsonmyelomacells
AT hidayettutun investigationofantiproliferativeeffectsofhomemadeandcommercialapplevinegarsonmyelomacells